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Development of Micro Analytical System of Hydroperoxides in Lipids

Research Project

Project/Area Number 63860011
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionTohoku University

Principal Investigator

MEGURO Hiroshi  Tohoku Univ., Agriculture, Professor, 農学部, 教授 (30005590)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Hiroaki  Tosho, Chief Researcher, 主任研究員
AKASAKA Kazuaki  Tohoku Univ., Agriculture, Associate Prof., 農学部, 助手 (10201881)
NISHIDA Yoshihiro  Tohoku Univ., Agriculture, Associate Prof., 農学部, 助手 (80183896)
OHRUI Hiroshi  Tohoku Univ., Agriculture, Assistant Prof., 農学部, 助教授 (20100050)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1989: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1988: ¥4,300,000 (Direct Cost: ¥4,300,000)
Keywordshydroperoxide / dertermination / fluorometry / HPLC / diphenyl pyrenyl phosphine / diphenylー1-pyrenylphosphine / high performance liquid chromatography / triacyl glycerol / ジフェニルー1ーピレニルホスフィン / ヒドロペルオキシド / 蛍光分析システム
Research Abstract

Extremely sensitive fluorometry of lipid hydroperoxides (HPO) in biological samples were developed using diphenyl-1-pyrenylphosphine (DPPP), a novel reagent synthesized us.
1. A batch method is developed to determine total HPO in edible fats and oils. The mixture of the reagent and sample solutions was reacted at 60^゚C for 60 min in the dark. After the reaction, DPPP oxide was determined by fluorometry. By the method, subnano mole level of lipid HPO could be determined with mg order of a sample.
2. HPLC systems are developed by using post column detection unit which composed of a pump to send the reagent solution, reaction coil and a fluorometer. By the systems, HPO were determined at pico mole level.
(1) Triacylglycerol (TG) HPO : TG-monoHPO were separated to several peaks on their partition numbers on an ODS colomn. On a phenylated silicagel column, they gave a single peak to made possible to determined total TG-monoHPO.
(2) Phosphatidylcholine (PC)- and Phosphatidylethanolamine (PE) -HPO : PC- and PE-HPO were separated by two columns, a silicagel and an aminopropyl columns, connected directly. This system was applicable to determine PC-HPO in plasma samples.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Kazuaki AKASAKA: "AN AROMATIC PHOSPHINE REAGENT FOR THE HPLCーFLUORESCENCE DETERMINATION OF HYDROPEROXIDES ー DETERMINATION OF PHOSPHATIDYLCHOLINE HYDROPEROXIDES IN HUMAN PLASMA ー" ANALYTICAL LETTERS. 21. 965-975 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroshi MEGURO: "Development of new fluorometrical reagents applicable to food and biological materials" Proceedings of the Third KoreaーJapan Joint Symposium on Analytical Chemistry. 153-158 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroshi MEGURO: "Determination of Hydroperoxides with Fluorometric Reagent Diphenylー1ーpyrenylphosphine" METHODS IN ENZYMOLOGY. 186. 157-161 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kazuaki AKASAKA: "A Simple Fluoromety of Hydroperoxides in Oils and Foods" Agric.Biol.Chem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kazuaki AKASAKA: "Fluorometric Determination of Triacylglycerol Hydroperoxides at Picomole Levels by HPLC PostーColumn Method with Diphenylー1ーpyrenylphosphine" J.Chromatogr.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kazuaki AKASAKA: "An aromatic phosphine reagent for the hplc-fluorescence determination of hydroperoxides -determination of phosphatidylcholine hydroperoxides in human plasma -" Analytical Letters. 21 (6). 965-975 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroshi MEGURO: "Development of new fluorometrical reagents applicable to food and biological materials" Proceedings of the Third Korea-Japan Joint Symposium on Analytical Chemistry. 153-158 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroshi MEGURO: "Determination of Hydroperoxides with Fluorometric Reagent Diphenyl-1-pyrenylphosphine" Methods in Enzymoligy. 186. 157-161 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kazuaki AKASAKA: "A Simple Fluoromety of Hydroperoxides in Oils and Foods" Agric. Biol. Chem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kazuaki AKASAKA: "Fluorometric Determination of Triacylglycerol Hydroperoxides at Picomole Levels by HPLC Post-Column Method with Diphenyl-1-pyrenylphosphine" J. Chromatogr.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroshi MEGURO: "Determination of Hydroperoxides with Fluorometric Reagent Diphenylー1ーpyrenylphosphine" METHODS IN ENZYMOLOGY. 186. 157-161 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kazuaki AKASAKA: "A Simple Fluoromety of Hydroperoxides in Oils and Foods" Agric.Biol.Chem.

    • Related Report
      1990 Annual Research Report
  • [Publications] Kazuaki AKASAKA: "Fluorometric Determination of Triacylglycerol Hydroperoxides at Picomole Levels by HPLC PostーColumn Method with Diphenylー1ーpyrenylphosphine" J.Chromatogr.

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroshi Meguro: "Development of new fluorometrical reagents applicable to food and biological materials" Proceedings of the Third Korea-Japan Joint Symposium on Analytical Chemistry(講演要旨集). 153-158 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kazuaki AKASAKA: ANALYTICAL LETTERS. 21(6). 965-975 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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