Signaling Functions of Reactive Oxygen Species

2012 Fiscal Year Final Research Report

• Project Area: Signaling functions of reactive oxygen species
• Project/Area Number: 20117001
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University (2013); Kumamoto University (2008-2012)
• Principal Investigator: AKAIKE Takaaki 熊本大学, 大学院・生命科学研究部, 教授 (20231798)
• Co-Investigator(Kenkyū-buntansha): SUMIMOTO Hideki 九州大学, 医学研究院, 教授 (30179303) ; UCHIDA Koji 名古屋大学, 生命農学研究科, 教授 (40203533) ; MATSUMOTO Akio 千葉大学, 医学研究科, 准教授 (60437308) ; URANO Yasuteru 東京大学, 医学研究科, 教授 (20292956) ; ARIMOTO Hirokazu 東北大学, 生命科学研究科, 教授 (60262789) ; ITOH Ken 弘前大学, 医学研究科, 教授 (10323289)
• Co-Investigator(Renkei-kenkyūsha): SHIMOKAWA Hiroaki 東北大学, 医学系研究科, 教授 (00235681) ; TSUTSUI Hiroyuki 北海道大学, 医学研究科, 教授 (70264017) ; UEHARA Takashi 岡山大学, 医歯薬学研究科, 教授 (00261321) ; KAMATA Hideaki 広島大学, 医歯薬学総合研究科, 准教授 (10233925) ; NISHIDA Motohiro 九州大学, 薬学研究院, 准教授 (90342641)
• Project Period (FY): 2008-11-13 – 2014-03-31
• Keywords: 活性酸素 / シグナル伝達 / 酸化ストレス / 生理活性 / ケミカルバイオロジー

Research Abstract

Signaling functions of reactive oxygen species have been explored by this research project from the view points of molecules, cells to individuals, resulting in publication of a number of original articles in high-impact journals. Obtained achievements were also widely distributed through internet, newsletters, technical seminar, and international symposiums organized by this esearch group.

Research Products

• [Journal Article] Nitrated cyclic GMP modulates guard cell signaling in Arabidopsis (2013)
  • Author(s): Joudoi T, Shichiri Y, Kamizono N, Akaike T, Sawa T, Yoshitake J, Yamada N, and Iwai S
  • Journal Title: Plant Cell Volume: 25 Pages: 558-71
  • Peer Reviewed
• [Journal Article] Redox signaling by 8-nitro-cyclic guanosine monophosphate: nitric oxide-and reactive oxygen species-derived electrophilic messenger (2013)
  • Author(s): Fujii S, and Akaike T
  • Journal Title: Antioxid Redox Signal Volume: (in press)
  • Peer Reviewed
• [Journal Article] Regulation of redox signalling by an electrophilic cyclic nucleotide (2013)
  • Author(s): Akaike T, Nishida M, and Fujii S
  • Journal Title: J Biochem Volume: 153 Pages: 131-8
  • Peer Reviewed
• [Journal Article] Nitric oxide/soluble guanylyl cyclase signaling mediates depolarization-induced protection of rat mesencephalic dopaminergic neurons from MPP+ cytotoxicity (2013)
  • Author(s): Kurauchi Y, Hisatsune A, Isohama Y, Sawa T, Akaike T, and Katsuki H
  • Journal Title: Neurosci Volume: (in press)
  • Peer Reviewed
• [Journal Article] S-Guanylation proteomics for redox-based mitochondrial signaling (2013)
  • Author(s): Rahaman MM, Sawa T, Ahtesham AK, Khan S, Inoue H, Irie A, Fujii S, and Akaike T
  • Journal Title: Antioxid Redox Signal Volume: (in press)
  • Peer Reviewed
• [Journal Article] Hydrogen sulfide anion regulates redox signaling via electrophile sulfhydration (2012)
  • Author(s): Nishida M, Sawa T, Kitajima N, Ono K, Inoue H, Ihara H, Motohashi H, Yamamoto M, Suematsu M, Kurose H, van der Vliet A, Freeman BA, Shibata T, Uchida K, Kumagai Y, and Akaike T
  • Journal Title: Nature Chem Biol Volume: 8 Pages: 714-24
  • Peer Reviewed
• [Journal Article] Keapl degradation by autophagy for the maintenance of redox homeostasis (2012)
  • Author(s): Taguchi K, Fujikawa N, Komatsu M, Ishii T, Unno M, Akaike T, Motohashi H, and Yamamoto M
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Pages: 13561-6
  • Peer Reviewed
• [Journal Article] Highly activatable and environment-insensitive optical highlighters for selective spatiotemporal imaging of target proteins (2012)
  • Author(s): Kobayashi T, Komatsu T, Kamiya M Campos C, Gonzalez-Gaitan M, Terai T, Hanaoka K, Nagano T, Urano Y
  • Journal Title: J Am Chem Soc Volume: 134 Pages: 11153-60
  • Peer Reviewed
• [Journal Article] RRedox signal-mediated sensitization of transient receptor potential melastatin 2 (TRPM2) to temperature affects macrophage functions (2012)
  • Author(s): Kashio M, Sokabe T, Shintaku K, Uematsu T, Fukuta N, Kobayashi N, Mori Y, Tominaga M
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Pages: 6745-50
  • Peer Reviewed
• [Journal Article] Interleukin-11 links oxidative stress and compensatory proliferation (2012)
  • Author(s): Nishina T, Komazawa-Sakon S, Yanaka S, Piao X, Zheng DM, Piao JH, Kojima Y, Yamashina S, Sano E, Putoczki T, Doi T, Ueno T, Ezaki J, Ushio H, Ernst M, Tsumoto K, Okumura K, and Nakano H
  • Journal Title: Sci Signal Volume: 5 Pages: ra5
  • Peer Reviewed
• [Journal Article] c-FLIP maintains tissue homeostasis by preventing apoptosis and programmed necrosis (2012)
  • Author(s): Piao, X, Komazawa-Sakon S, Nishina T, Koike M, Piao JH, Ehlken H, Kunihara H, Hara M, Van Rooijen N, Schutz G, Ohmuraya M, Uchiyama Y, Yagita H, Okumura K, He YW, and Nakano H
  • Journal Title: Sci Signal Volume: 5 Pages: ra93
  • Peer Reviewed
• [Journal Article] TRPA1 underlies a sensing mechanism for O_2 (2011)
  • Author(s): Takahashi N, Kuwaki T, Kiyonaka S, Numata T, Kozai D, Mizuno Y, Yamamoto S, Naito S, Knevels E, Carmeliet P, Oga T, Kaneko S, Suga S, Nokami T, Yoshida J, and Mori Y
  • Journal Title: Nature Chem Biol Volume: 7 Pages: 701-11
  • Peer Reviewed
• [Journal Article] Thioredoxin mediates oxidation-dependent phosphorylation of CRMP2 and growth cone collapse (2011)
  • Author(s): Morinaka A, Yamada M, Itofusa R, Funato Y, Yoshimura Y, Nakamura F, Yoshimura T, Kaibuchi K, Goshima Y, Hoshino M, Kamiguchi H, and Miki H
  • Journal Title: Sci Signal Volume: 4 Pages: ra26
  • Peer Reviewed
• [Journal Article] Structural basis for interaction between the conserved cell polarity proteins Inscuteable and Leu-Gly-Asn repeat-enriched protein (LGN) (2011)
  • Author(s): Yuzawa S, Kamakura S, Iwakiri Y, Hayase J, Sumimoto H
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Pages: 19210-15
  • Peer Reviewed
• [Journal Article] Polarity proteins Bem1 and Cdc24 are components of the filamentous fungal NADPH oxidase complex (2011)
  • Author(s): Takemoto D, Kamakura S, Saikia S, Becker Y, Wrenn R, Tanaka A, Sumimoto H, and Scott B
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Pages: 2861-6
  • Peer Reviewed
• [Journal Article] Heterologous down-regulation of angiotensin type 1 receptors by purinergic P2Y2 receptor stimulation through S-nitrosylation of NF-kappaB (2011)
  • Author(s): Nishida M, Ogushi M, Suda R, Toyotaka M, Saiki S, Kitajima N, Nakaya M, Kim KM, Ide T, Sato Y, Inoue K, and Kurose H
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Pages: 6662-7
  • Peer Reviewed
• [Journal Article] On-off system for PI3-kinase-Akt signaling through S-nitrosylation of phosphatase with sequence homology to tensin (PTEN) (2011)
  • Author(s): Numajiri N, Takasawa K, Nishiya T, Tanaka H, Ohno K, Hayakawa W, Asada M, Matsuda H, Azumi K, Kamata H, Nakamura T, Hara H, Minami M, Lipton SA, and Uehara T
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Pages: 10349-54
  • Peer Reviewed
• [Journal Article] Phosphorylation of the nicotiana benthamiana WRKY8 transcription factor by MAPK functions in the defense response (2011)
  • Author(s): Ishihama N, Yamada R, Yoshioka M, Katou S, Yoshioka H
  • Journal Title: Plant Cell Volume: 23 Pages: 1153-70
  • Peer Reviewed
• [Journal Article] The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keapl (2010)
  • Author(s): Komatsu M, Kurokawa H, Waguri S, Taguchi K, Kobayashi A, Ichimura Y, Sou YS, Ueno I, Sakamoto A, Tong KI, Kim M, Nishito Y, Iemura S, Natsume T, Ueno T, Kominami E, Motohashi H, Tanaka K, and Yamamoto M
  • Journal Title: Nature Cell Biol Volume: 12 Pages: 213-23
  • Peer Reviewed
• [Journal Article] Different modes of ubiquitination of the adaptor TRAF3 selectively activate the expression of type I interferons and proinflammatory cytokines (2010)
  • Author(s): Tseng PH, Matsuzawa A, Zhang W, Mino T, Vignali DA, and Karin M
  • Journal Title: Nature Immunol Volume: 11 Pages: 70-5
  • Peer Reviewed
• [Journal Article] Nuclear IKKbeta is an adaptor protein for IkappaBalpha ubiquitination and degradation in UV-induced NF-kappaB activation (2010)
  • Author(s): Tsuchiya Y, Asano T, Nakayama K, Kato T, Jr., Karin M, and Kamata H
  • Journal Title: Mol Cell Volume: 39 Pages: 570-82
  • Peer Reviewed
• [Journal Article] Assembly pathway of the Mammalian proteasome base subcomplex is mediated by multiple specific chaperones (2009)
  • Author(s): Kaneko T, Hamazaki J, Iemura S, Sasaki K, Furuyama K, Natsume T, Tanaka K, and Murata S
  • Journal Title: Cell Volume: 137 Pages: 914-25
  • Peer Reviewed
• [Journal Article] Ubiquitin-like sequence in ASK1 plays critical roles in the recognition and stabilization by USP9X and oxidative stress-induced cell death (2009)
  • Author(s): Nagai H, Noguchi T, Homma K, Katagiri K, Takeda K, Matsuzawa A, and Ichijo H
  • Journal Title: Mol Cell Volume: 36 Pages: 805-18
  • Peer Reviewed
• [Journal Article] Selective molecular imaging of viable cancer cells with pH-activatable fluorescence probes (2009)
  • Author(s): Urano Y, Asanuma D, Hama Y, Koyama Y, Barrett T, Kamiya M, Nagano T, Watanabe T, Hasegawa A, Choyke PL, and Kobayashi H
  • Journal Title: Nature Med Volume: 15 Pages: 104-9
  • Peer Reviewed
• [Journal Article] NF-kB2 (p100) limits TNF-a-induced osteoclastogenesis (2009)
  • Author(s): Tanaka S, and Nakano H
  • Journal Title: J Clin Invest Volume: 119 Pages: 2879-81
  • Peer Reviewed
• [Presentation] Electrophilic modification of H-Ras underlies development of heart failure after myocardial infarction (2012)
  • Author(s): Nishida M
  • Organizer: Fourth International Symposium on Signaling Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Kyushu University, Fukuoka, Japan
  • Year and Date: 2012-12-17
• [Presentation] Mass spectrometric identification of unique polysulfides in biological systems (2012)
  • Author(s): Sawa T, Ida T, Akaike T
  • Organizer: Fourth International Symposium on Signaling Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Kyushu University, Fukuoka, Japan
  • Year and Date: 2012-12-17
• [Presentation] Keapl-Nrf2 system in metabolic reprogramming of cancer cells (2012)
  • Author(s): Motohashi H
  • Organizer: Fourth International Symposium on Signaling Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Kyushu University, Fukuoka, Japan
  • Year and Date: 2012-12-17
• [Presentation] The role of ribosome interacting protein GCN1L1 in oxidative stress-mediated Nrf2 activation (2012)
  • Author(s): Itoh K
  • Organizer: Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Kyushu University, Fukuoka, Japan
  • Year and Date: 2012-12-17
• [Presentation] Electrophilic ligands as a trigger of pro-inflammatory response (2012)
  • Author(s): Uchida K
  • Organizer: Fourth International Symposium on Signaling Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Kyushu University, Fukuoka, Japan
  • Year and Date: 2012-12-17
• [Presentation] Redox physiology of TRP channels (2012)
  • Author(s): Mori Y
  • Organizer: Fourth International Symposium on Signaling Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Kyushu University, Fukuoka, Japan
  • Year and Date: 2012-12-17
• [Presentation] Oxidative stress maintains tissue homeostasis by inducing compensatory proliferation (2012)
  • Author(s): Nakano H
  • Organizer: Fourth International Symposium on Signaling Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Kyushu University, Fukuoka, Japan
  • Year and Date: 2012-12-17
• [Presentation] 生体の酸化還元状態・環境を感知するチャネル機構:TRP (2012)
  • Author(s): 森泰生
  • Organizer: 文部科学省新額術領域研究「活性酸素のシグナル伝達機能」特別公開シンポジウム
  • Place of Presentation: 沖縄かりゆしアーバンリゾート・ナハ、(沖縄)
  • Year and Date: 2012-06-18
• [Presentation] lectrophilic cellular signaling regulated by H2S-mediated sulfhydration (2011)
  • Author(s): Akaike T
  • Organizer: Third International Symposium on Signaling Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Showa Pharmaceutical University, Tokyo, Japan
  • Year and Date: 2011-05-14
• [Presentation] A novel modulatory 'on-off' system for PI 3-kinase-Akt signaling through S-nitrosylation (2011)
  • Author(s): Uehara T
  • Organizer: Third International Symposium on Signaling Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Showa Pharmaceutical University, Tokyo, Japan
  • Year and Date: 2011-05-14
• [Presentation] Regulatory crosstalk of redox homeostasis and anabolic metabolism by the Keap1-Nrf2 pathway (2011)
  • Author(s): Motohashi H
  • Organizer: Third International Symposium on Signaling Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Showa Pharmaceutical University, Tokyo, Japan
  • Year and Date: 2011-05-14
• [Presentation] Regulatory mechanisms of ROS-induced apoptosis and activation of the stress-responsive kinase ASK1 by the deubiquitinating enzyme USP9X (2010)
  • Author(s): Matsuzawa A
  • Organizer: Second International Symposium on Signaling Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2010-06-18
• [Presentation] Hepatocyte-specific c-Flip-deficient mice uncover a causal link between oxidative stress and tissue repair (2010)
  • Author(s): Nakano H
  • Organizer: Second International Symposium on Signaling Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2010-06-18
• [Presentation] Real-time imaging of various ROS and enzymatic activity in living cells byusing precisely developed novel fluorescence probes (2009)
  • Author(s): Urano Y
  • Organizer: International Symposium 2009 on Signaling Functions of Reactive Oxygen Species, Grants-in-Aid for Scientific Research on Innovative Areas, MEXT
  • Place of Presentation: Hotel Greenpia Minami-aso, Kumamoto, Japan
  • Year and Date: 2009-07-18
• [Book] 活性酸素・ガス状分子による恒常性制御と疾患:酸化ストレス応答と低酸素センシングの最新知見からがん、免疫、代謝・呼吸・循環以上、神経変性との関わりまで.実験医学増刊 (2012)
  • Author(s): 山本雅之(監修)、赤池孝章、一條秀憲、森泰生(編集)
  • Total Pages: 217
  • Publisher: 羊土社
• [Book] 病態解明に迫る活性酸素シグナルと酸化ストレス:癌、神経変性疾患、循環・代謝異常にかかわるレドックス制御機構と最新の技術開発.実験医学増刊 (2009)
  • Author(s): 谷口直之(監修)、赤池孝章、鈴木敬一郎、内田浩二(編集)
  • Total Pages: 241
  • Publisher: 羊土社
  • URL: http://www.ros-signal.jp/