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2010 Fiscal Year Annual Research Report

過渡的複合体が関わる生命現象の統合的理解-生理的準安定状態を捉える新技術-

Administrative Group

Project AreaIntegrative understanding of biological processes mediated by transient macromolecular complexes; New technology for visualizing physiologically metastable states.
Project/Area Number 21121001
Research InstitutionThe University of Tokyo

Principal Investigator

嶋田 一夫  東京大学, 大学院・薬学系研究科, 教授 (70196476)

Co-Investigator(Kenkyū-buntansha) 神田 大輔  九州大学, 生体防御医学研究所, 教授 (80186618)
Keywords構造生物学 / 核磁気共鳴法 / X線結晶構造解析 / 一分子計測学 / ケミカル・バイオロジ
Research Abstract

本年度の総括班の活動は、班会議開催(平成22年5月24,25,26日於リゾナーレ小淵沢)
および多くの研究者に新学術領域の研究内容を公表するために、通常行われているシンポジウムの開催という手法を取らず、参加者の大きな学会でのワークショップおよびシンポジウムを下記のように企画した。
■日本蛋白質科学会第10回年会【会期】平成22年6月16日(水)16:00~18:30【会場】札幌コンベンションセンターB会場:中ホール【ワークショップ】過渡的複合体の視点から生命現象を捉えなおす
■第49回NMR討論会【会期】平成22年11月15日(月),16日(火),17日(水)【会場】タワーホール船堀【共催】新学術領域研究「過渡的複合体が関わる生命現象の統合的理解」
■日本化学会第91回春季年会【会期】平成23年3月27日(土)【会場】神奈川大学【ワークショップ】過渡的複合体を含む生命現象解明を目指した化学的アプローチ
また、若手育成援助として下記のミーティングを協賛した。
■「先導的若手NMRスペシャリスト育成プログラム」第1回研修会【共催】日本核磁気共鳴学会、日本分光学会、新学術領域「過渡的複合体」【後援】先端研究施設共用促進事業【協力】日本電子、アジレントテクノロジー、ブルカーバイオスピン【会期】2010年12月2日(木)~4日(土)【会場】琵琶湖リゾートクラブ(滋賀県守山市)【定員】20名
【URL】http://www.protein.osaka-u.ac.jp/biophys/ja_events.html

  • Research Products

    (30 results)

All 2011 2010

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (16 results)

  • [Journal Article] Structure determination of a protein assembly by amino acid selective cross-saturation2011

    • Author(s)
      Eiji Kanamori, Shunsuke Igarashi, Masanori Osawa, Yoshifumi Fukunishi, Ichio Shimada, Haruki Nakamura
    • Journal Title

      Proteins

      Volume: 79 Pages: 179-190

    • Peer Reviewed
  • [Journal Article] HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling : a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment2011

    • Author(s)
      Koh Takeuchi, Maayan Gal, Hideo Takahashi, Ichio Shimada, Gerhard Wagner
    • Journal Title

      J.Biomol.NMR

      Volume: 49 Pages: 17-26

    • Peer Reviewed
  • [Journal Article] Production of isotopically labeled heterologous proteins in non-E.coli prokaryotic and eukaryotic cells2010

    • Author(s)
      Hideo Takahashi, Ichio Shimada
    • Journal Title

      J.Biomol.NMR

      Volume: 46 Pages: 3-10

    • Peer Reviewed
  • [Journal Article] Real-time assay method of lipid extraction activity2010

    • Author(s)
      Toshihiko Sugiki, Hideo Takahashi, Michiko Nagasu, Kentaro Hanada, Ichio Shimada
    • Journal Title

      Anal.Biochem.

      Volume: 399 Pages: 162-167

    • Peer Reviewed
  • [Journal Article] Structural Basis for Platelet Anti-aggregation by AT1-Receptor Antagonist Losartan via Glycoprotein VI2010

    • Author(s)
      Katsuki Ono, Hiroshi Ueda, Yoshitaka Yoshizawa, Daisuke Akazawa, Ryuji Tanimura, Ichio Shimada, Hideo Takahashi
    • Journal Title

      J.Med.Chem.

      Volume: 53 Pages: 1917-1922

    • Peer Reviewed
  • [Journal Article] Direct determination of the insulin-insulin receptor interface using transferred cross-saturation experiments2010

    • Author(s)
      Takefumi Nakamura, Hideo Takahashi, Mitsuo Takahashi, Nobuhisa Shimba, Ei-ichiro Suzuki, Ichio Shimada
    • Journal Title

      J.Med.Chem.

      Volume: 53 Pages: 2087-2093

    • Peer Reviewed
  • [Journal Article] Structural basis underlying the dual gate properties of KcsA2010

    • Author(s)
      Shunsuke Imai, Masanori Osawa, Koh Takeuchi, Ichio Shimada
    • Journal Title

      Proc.Natl.Acad.Sci.USA.

      Volume: 107 Pages: 6216-6221

    • Peer Reviewed
  • [Journal Article] Two-state conformational equilibrium of the hyaluronan-binding domain regulates adhesiveness of CD44 under flow condition2010

    • Author(s)
      Shinji Ogino, Noritaka Nishida, Ryo Umemoto, Miho Suzuki, Mitsuhiro Takeda, Hiroaki Terasawa, Joji Kitayama, Masanori Matsumoto, Haruko Hayasaka, Masayuki Miyasaka, Ichio Shimada
    • Journal Title

      Structure

      Volume: 18 Pages: 649-656

    • Peer Reviewed
  • [Journal Article] Precise structural determination of weakly binding peptides by utilizing dihedral angle constraints2010

    • Author(s)
      Yumiko Mizukoshi, Michiko Nagasu, Ichio Shimada, Hideo Takahashi
    • Journal Title

      J.Biomol.NMR

      Volume: 46 Pages: 299-305

    • Peer Reviewed
  • [Journal Article] NMR analyses of the interaction between CCR5 and its ligand using functional reconstitution of CCR5 in lipid bilayers2010

    • Author(s)
      Chie Yoshiura, Yutaka Kofuku, Takumi Ueda, Yoko Mase, Mariko Yokogawa, Masanori Osawa, Yuya Terashima, Kouji Matsushima, Ichio Shimada
    • Journal Title

      J.Am.Chem.Soc.

      Volume: 132 Pages: 6768-6777

    • Peer Reviewed
  • [Journal Article] Theoretical analyses of the transferred cross-saturation method2010

    • Author(s)
      Masahiko Matsumoto, Takumi Ueda, Ichio Shimada
    • Journal Title

      J.Magn.Reson.

      Volume: 205 Pages: 114-124

    • Peer Reviewed
  • [Journal Article] Backbone resonance assignments for the ligand binding subunit of the histidine permease complex (HisJ) from Escherichia coli, under histidine-bound and unbound states.2010

    • Author(s)
      Igarashi S, Osawa M, Ozawa S, Shimada I.
    • Journal Title

      Biomol NMR Assign.

      Volume: 4 Pages: 7-20

    • Peer Reviewed
  • [Journal Article] NMR structure of the calponin homology domain of human IQGAP1 and its implications for the actin recognition mode2010

    • Author(s)
      Ryo Umemotoa, Noritaka Nishida, Shinji Oginoa, Ichio Shimada
    • Journal Title

      J.Biomol.NMR

      Volume: 48 Pages: 59-64

    • Peer Reviewed
  • [Journal Article] Quantitative characterization of the Tob interactions provides the thermodynamic basis on the translation termination-coupled deadenylase regulation2010

    • Author(s)
      Lin Ruan, Masanori Osawa, Nao Hosoda, Shunsuke Imai, Asako Machiyama, Toshiaki Katada, Shin-ichi Hoshino, Ichio Shimada
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 27624-27631

    • Peer Reviewed
  • [Presentation] NMR法によるactinとactin結合タンパク質との相互作用解析法の開発2010

    • Author(s)
      黄淑嫻、梅本良、上田太郎、西田紀貴、嶋田一夫
    • Organizer
      第33回日本分子生物学会年会 第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド,兵庫県
    • Year and Date
      2010-12-08
  • [Presentation] 高分子量蛋白質の構造解析に適した安定同位体ラベル法および測定法の開発2010

    • Author(s)
      竹内恒、高橋栄夫、嶋田一夫、Gerhard Wagner
    • Organizer
      第49回NMR討論会
    • Place of Presentation
      船堀タワーホール,東京都
    • Year and Date
      2010-11-15
  • [Presentation] Dynamical aspects of membrane proteins2010

    • Author(s)
      嶋田一夫
    • Organizer
      Max Planck Institute seminar
    • Place of Presentation
      Gottingen, Germany
    • Year and Date
      2010-10-21
  • [Presentation] Dynamical aspects of membrane proteins2010

    • Author(s)
      嶋田一夫
    • Organizer
      Technische Universitat Munchen/Helmholtz Zentrum Munchen seminar
    • Place of Presentation
      Munchen, Germany
    • Year and Date
      2010-10-20
  • [Presentation] Dynamical aspects of membrane proteins by NMR2010

    • Author(s)
      嶋田一夫
    • Organizer
      The International Network of Protein Engineering Centres (INPEC) 2010
    • Place of Presentation
      Uppsala, Sweden
    • Year and Date
      2010-10-08
  • [Presentation] The 2nd International Symposium on Drug Discovery and Design by NMR2010

    • Author(s)
      嶋田一夫
    • Organizer
      Functional analyses of membrane proteins by NMR
    • Place of Presentation
      横浜市立大学,神奈川県
    • Year and Date
      2010-09-30
  • [Presentation] NMRを用いた膜タンパク質の機能解明2010

    • Author(s)
      嶋田一夫
    • Organizer
      日本分光学会NMR分光部会シンポジウム
    • Place of Presentation
      東京大学,東京都
    • Year and Date
      2010-09-08
  • [Presentation] Dynamical aspects of membrane proteins2010

    • Author(s)
      嶋田一夫
    • Organizer
      Frankfurt University seminar
    • Place of Presentation
      Frankfurt, Germany
    • Year and Date
      2010-09-06
  • [Presentation] Dynamical aspects of membrane proteins2010

    • Author(s)
      嶋田一夫
    • Organizer
      International Conference on Magnetic Resonance in Biological Systems
    • Place of Presentation
      Cairns, Australia
    • Year and Date
      2010-08-23
  • [Presentation] NMRによるケモカイン受容体・ケモカイン相互作用解析2010

    • Author(s)
      嶋田一夫
    • Organizer
      第34回阿蘇シンポジウム
    • Place of Presentation
      阿蘇リゾートグランヴィリオホテル,熊本県
    • Year and Date
      2010-07-30
  • [Presentation] -超高磁場が拓く生体系NMR:最新技術と応用-膜タンパク質のダイナミックスと機能2010

    • Author(s)
      嶋田一夫
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Place of Presentation
      大阪大学,大阪府
    • Year and Date
      2010-07-29
  • [Presentation] NMRによる膜タンパク質の機能解析2010

    • Author(s)
      嶋田一夫
    • Organizer
      日本学術振興会、回折構造生物学第169委員会、第32回研究会
    • Place of Presentation
      ゆうぽうと,東京都
    • Year and Date
      2010-07-20
  • [Presentation] Structural bases of the interactions between chemokines and their G-protein-coupled receptors2010

    • Author(s)
      嶋田一夫
    • Organizer
      Symposium on Structure and Folding of Disease Related Proteins
    • Place of Presentation
      Seoul National University, Korea
    • Year and Date
      2010-07-10
  • [Presentation] Structural bases of the interactions between chemokines and their G-protein-coupled receptors2010

    • Author(s)
      嶋田一夫
    • Organizer
      EUROMAR/ISMAR
    • Place of Presentation
      Florence, Italy
    • Year and Date
      2010-07-06
  • [Presentation] 過渡的複合体が関わる生命現象の統合的理解2010

    • Author(s)
      嶋田一夫
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌コンベンションセンター,北海道
    • Year and Date
      2010-06-16
  • [Presentation] Functional analyses of membrane proteins by NMR2010

    • Author(s)
      嶋田一夫
    • Organizer
      COLD SPRING HARBOR ASIA CONFERENCES
    • Place of Presentation
      Suzhou, China
    • Year and Date
      2010-05-13

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Published: 2012-07-19  

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