2020 Fiscal Year Annual Research Report
Scrap& build of synapse towards understanding neuropsychiatric disorders
| Project Area | Dynamic regulation of brain function by Scrap & Build system |
|---|
| Project/Area Number |
16H06463
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
内匠 透 神戸大学, 医学研究科, 教授 (00222092)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
萬代 研二 北里大学, 医学部, 教授 (50322186)
|
|---|
| Project Period (FY) |
2016-06-30 – 2021-03-31
|
| Keywords | PSD / プロテオミクス |
|---|
| Outline of Annual Research Achievements |
The postsynaptic density (PSD) is a huge protein complex beneath the postsynaptic membrane of excitatory synapses. The number, shape, and plasticity of synapses are altered during development. However, the dynamics of synaptic protein composition across development has not been fully understood. Here we show alterations of PSD protein composition in mouse and primate brains (monkey and human) during development. Proteins involved in synapse regulation are enriched in differentially expressed (288 decreased and 267 increased) PSD proteins in 2-week-old mice. Analysis of the transcriptome datasets suggests that changes in PSD protein abundance are transcriptionally regulated and also take place in primate brain during the perinatal period. This perinatal alteration of PSD composition is likely to be defective in brains of autism spectrum disorder (ASD) patients. Finally, we demonstrate that after the juvenile period the brain of common marmoset (Callithrix jacchus) also shows changes in PSD composition. The alteration of PSD composition after postnatal 2 months is distinct from that observed in mice. Our results provide a comprehensive architecture of the remodeling of PSD composition across development, which may explain the molecular basics of synapse maturation and the pathology of psychiatric disorders, such as ASD.
|
| Research Progress Status |
令和2年度が最終年度であるため、記入しない。
|
| Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
|
