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2020 Fiscal Year Final Research Report

Control of proton motive force components by ion transport system

Planned Research

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Project AreaNew Photosynthesis : Reoptimization of the solar energy conversion system
Project/Area Number 16H06558
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionTohoku University

Principal Investigator

Uozumi Nobuyuki  東北大学, 工学研究科, 教授 (40223515)

Co-Investigator(Kenkyū-buntansha) 川崎 寿  東京大学, 生物生産工学研究センター, 特任教授 (90349788)
池内 昌彦  東京大学, 大学院総合文化研究科, 名誉教授 (20159601)
Project Period (FY) 2016-06-30 – 2021-03-31
Keywords膜輸送体 / 膜電位 / プロトン駆動力 / イオン / チラコイド膜
Outline of Final Research Achievements

We showed that Arabidopsis chloroplasts possess both influx and efflux of K-transport activities in KEA1-KEA6. In addition, the mutant, dpgr (KEA3-G422R), exhibited increased spectifc transport activity, compared with that of the wild type KEA3, consistent with physiological results. We also detected the Na and H exchange transport activity of the cyanobacterial Na/H antiporters, NhaS1-NhaS6. Some of NhaS located in the thylakoid membranes, and played an essential role in photosynthetic function under high light conditions.

Free Research Field

電気生理学

Academic Significance and Societal Importance of the Research Achievements

光合成の明反応は葉緑体や藍藻で行われており,両者の長所を生かして詳細な検討をすすめた.チラコイド膜におけるプロトン駆動力の形成に必要なNaやK輸送体を同定して,イオン輸送活性を明らかにした.このことにより,チラコイドの膜電位形成には,H以外のNaやKなどの陽イオンが関与しており,その輸送体の実体の一部が明らかにされた.これらの輸送体が光エネルギーの変換でチラコイド膜に蓄積される電気化学エネルギーの生成と変換に重要な役割を担うことが示された.

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Published: 2022-01-27  

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