2010 Fiscal Year Final Research Report
The structure-function relationship of the dermonecrosis-inducing toxins and their roles in the bacterial infections.
| Project Area | Matrix of Infection Phenomena |
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| Project/Area Number |
18073012
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Osaka University |
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Principal Investigator |
HORIGUCHI Yasuhiko Osaka University, 微生物病研究所, 教授 (00183939)
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| Project Period (FY) |
2006 – 2010
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| Keywords | パスツレラ毒素 / 結晶構造 |
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| Research Abstract |
We determined the crystal structure of Pasteurella multocida toxin, a member of the dermonecrosis-inducing toxin, from which an enzyme activity of the toxin was predicted. In addition, by analyzing the domain-structure organization, we found that C1 domain of the toxin was involved in its localization to the plasma membrane. Rat monoclonal antibodies against a product of the enzymic action of the toxin were also developed. These antibodies were considered useful for detection of the toxin action both in vitro and in situ.
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[Journal Article] Clostridium perfringens enterotoxin interacts with claudins via electrostatic attraction.2010
Author(s)
Kimura, J., H.Abe, S.Kamitani, H.Toshima, A.Fukui, Miyake, M.Y.Kamata,Y.Sugita-Konishi, S.Yamamoto, Y.Horiguchi
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Journal Title
The Journal of biological chemistry 285
Pages: 401-408
Peer Reviewed
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[Journal Article] Human herpesvirus 6 ganciclovir-resistant strain with amino acid substitutions associated with the death of an allogeneic stem cell transplant recipient.2009
Author(s)
Isegawa, Y., J.Hara, K.Amo, Y.Osugi, M.Takemoto, K.Yamanishi, R.Fukunaga, M.Shibata, A.Ohshima, Y.Horiguchi, N.Sugimoto
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Journal Title
J Clin Virol 44
Pages: 15-19
Peer Reviewed
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[Journal Article] Domain mapping of a claudin-4 modulator, the C-terminal region of C-terminal fragment of Clostridium perfringens enterotoxin, by site-directed mutagenesis.2008
Author(s)
Takahashi, A., E.Komiya, H.Kakutani, T.Yoshida, M.Fujii, Y.Horiguchi, H.Mizuguchi, Y.Tsutsumi, S.Tsunoda, N.Koizumi, K.Isoda, K.Yagi, Y.Watanabe, M.Kondoh.
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Journal Title
Biochem Pharmacol 75
Pages: 1639-1648
Peer Reviewed
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[Journal Article] Characterization of the human herpesvirus 6 U69 gene product and identification of its nuclear localization signal.2008
Author(s)
Isegawa, Y., Y.Miyamoto, Y.Yasuda, K.Semi, K.Tsujimura, R.Fukunaga, A.Ohshima, Y.Horiguchi, Y.Yoneda, N.Sugimoto.
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Journal Title
J Virol 82
Pages: 710-718
Peer Reviewed
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[Journal Article] Role of tyrosine residues in modulation of claudin-4 by the C-terminal fragment of Clostridium perfringens enterotoxin2007
Author(s)
Harada, M., M.Kondoh, C.Ebihara, A.Takahashi, E.Komiya, M.Fujii, H.Mizuguchi, S.Tsunoda, Y.Horiguchi, K.Yagi, Y.Watanabe.
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Journal Title
Biochem Pharmacol 73
Pages: 206-214
Peer Reviewed
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[Journal Article] Enteropathogenic Escherichia coli, Shigella flexneri, and Listeria monocytogenes recruit a junctional protein, zonula occludens-1, to actin tails and pedestals.2007
Author(s)
Hanajima-Ozawa, M., T.Matsuzawa, A.Fukui, S.Kamitani, H.Ohnishi, A.Abe, Y.Horiguchi, M.Miyake.
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Journal Title
Infect Immun 75
Pages: 565-573
Peer Reviewed
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