Studies on odorant and pheromone receptor as a chemosensor for the external environmen

2010 Fiscal Year Final Research Report

• Project Area: Molecular interaction and modal shift of cellular sensors
• Project/Area Number: 18077001
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: 東原 和成 The University of Tokyo, 大学院・農学生命科学研究科, 教授 (00280925)
• Project Period (FY): 2006 – 2010
• Keywords: 匂い / フェロモン / 嗅覚 / センサー / 受容体 / チャネル / 昆虫 / マウス

Research Abstract

Animals are attracted by general odorants derived from food or by pheromones released from the opposite sex. The chemical information is detected by seven-transmembrane chemosensory receptors expressed by peripheral olfactory sensory neurons. In this project, we revealed the molecular mechanisms underlying chemosensory reception and the neural circuitry activated by the chemosensory receptors for a pheromone or a biologically important odorant that is crucial for survival or mating in various animal species.

Research Products

• [Journal Article] Enzymatic conversion of odorants in nasal mucus affects olfactory glomerular activation patterns and odor perception (2010)
  • Author(s): Nagashima, A., Touhara, K.
  • Journal Title: J.Neurosci. 30 Pages: 16391-16398
  • Peer Reviewed
• [Journal Article] Adiponectin and AdipoR1 regulate PGC-1alpha and mitochondria by Ca(2+) and AMPK/SIRT1 (2010)
  • Author(s): Iwabu M, Yamauchi T, Okada-Iwabu M, Sato K, Nakagawa T, Funata M, Yamaguchi M, Namiki S, Nakayama R, Tabata M, Ogata H, Kubota N, Takamoto I, Hayashi YK, Yamauchi N, Waki H, Fukayama M, Nishino I, Tokuyama K, Ueki K, Oike Y, Ishii S, Hirose K, Shimizu T, Touhara K, Kadowaki T.
  • Journal Title: Nature 464 Pages: 1313-1319
  • Peer Reviewed
• [Journal Article] The male mouse pheromone ESP1 enhances female sexual receptive behavior through a specific vomeronasal receptor (2010)
  • Author(s): Haga, S., Hattori, T., Sato, T., Sato, K., Matsuda, S., Kobayakawa, R., Sakano, H., Yoshihara, Y., Kikusui, T., Touhara, K.
  • Journal Title: Nature 466 Pages: 118-122
  • Peer Reviewed
• [Journal Article] Sensing odorants and pheromones with chemosensory receptors (2009)
  • Author(s): Touhara, K., Vosshall, L.B.
  • Journal Title: Annu.Rev.Physiol. 71 Pages: 307-332
• [Journal Article] Two chemoreceptors mediate the developmental effects of dauer pheromone in C.elegans (2009)
  • Author(s): Kim, K., Sato, K., Shibuya, M., Zeiger, D.M., Butcher, R.A., Ragains, J.R., Clardy, J., Touhara, K., Sengupta, P.
  • Journal Title: Science 326 Pages: 994-998
  • Peer Reviewed
• [Journal Article] Nasal airflow rate affects the sensitivity and pattern of glomerular odorant responses in the mouse olfactory bulb (2009)
  • Author(s): Oka, Y., Takai, Y., Touhara, K.
  • Journal Title: J.Neurosci. 29 Pages: 12070-12078
  • Peer Reviewed
• [Journal Article] Highly selective tuning of a silkworm olfactory receptor to a key mulberry leaf volatile (2009)
  • Author(s): Tanaka, K., Uda, Y., Ono, Y., Nakagawa, T., Suwa, M., Yamaoka, R., Touhara, K.
  • Journal Title: Current Biology 19 Pages: 881-890
  • Peer Reviewed
• [Journal Article] Insect olfactory receptors are heteromeric ligand-gated channels (2008)
  • Author(s): Sato, K., Pellegrino, M., Nakagawa, T., Nakagawa, T., Vosshall, L.B., Touhara, K.
  • Journal Title: Nature 452 Pages: 1002-1006
  • Peer Reviewed
• [Journal Article] Amino acids involved in conformational dynamics and G-protein coupling of an odorant receptor : targeting gain-of-function mutation (2008)
  • Author(s): Kato, A., Katada, S., Touhara, K.
  • Journal Title: J.Neurochem. 107 Pages: 1261-1270
  • Peer Reviewed
• [Journal Article] The mouse ESP family : sex and strain differences, and implications in the vomeronasal sensory system (2007)
  • Author(s): Kimoto, H., Sato, K., Nodari, F., Haga, S., Holy, T., Touhara, K.
  • Journal Title: Current Biology 17 Pages: 1879-1884
  • Peer Reviewed
• [Journal Article] Odorant receptor map in the mouse olfactory bulb : in vivo sensitivity and specificity of receptor-defined glomeruli (2006)
  • Author(s): Oka, Y., Katada, S., Omura, M., Suwa, M., Yoshihara, Y., Touhara, K.
  • Journal Title: Neuron 52 Pages: 857-869
  • Peer Reviewed
• [Presentation] Sensing odorant and pheromone. (2010)
  • Author(s): Touhara, K.
  • Organizer: Janelia Farm Conference
  • Place of Presentation: Janelia Farm, USA
  • Year and Date: 20100523-20100526
• [Presentation] Chemosensory receptor and behavior. (2010)
  • Author(s): Touhara, K.
  • Organizer: International Titisee Conferences
  • Place of Presentation: Titisee, Germany
  • Year and Date: 2010-10-14
• [Presentation] The ESP peptide family and its role in chemical communication. (2009)
  • Author(s): Touhara, K.
  • Organizer: European Chemoreception Research Organization Congress.
  • Place of Presentation: Sargenia, Italy
  • Year and Date: 20090924-20090927
• [Presentation] Chemosensory receptors for odors and pheromones. (2009)
  • Author(s): Touhara, K.
  • Organizer: International Congress of Physiological Sciences.
  • Place of Presentation: Kyoto
  • Year and Date: 20090727-20090801
• [Presentation] Vomeronasal reception of a sex peptide pheromone ESP1 in mice : the receptor, neural circuitry, and behavior. (2009)
  • Author(s): Touhara, K.
  • Organizer: 31st Annual meeting of association for chemoreception sciences
  • Place of Presentation: Florida, USA
  • Year and Date: 20090422-20090426
• [Presentation] Chemosensory receptor and behavior. (2009)
  • Author(s): Touhara, K.
  • Organizer: Keystone Symposium on Chemical Senses
  • Place of Presentation: CA, USA
  • Year and Date: 20090315-20090318
• [Presentation] A neural circuitry activated by a sex-specific peptide pheromone in mice : sexual communication via direct contact. (2008)
  • Author(s): Touhara, K.
  • Organizer: UK-Japan FoS symposium
  • Place of Presentation: France
  • Year and Date: 20081003-20081005
• [Presentation] Emerging view of insect olfactory receptor signaling. (2008)
  • Author(s): Touhara, K.
  • Organizer: International. Symposium on Olfaction and Taste (ISOT)
  • Place of Presentation: CA, USA
  • Year and Date: 20080721-20080728
• [Presentation] Molecular mechanisms underlying sex pheromone reception. (2007)
  • Author(s): Touhara, K.
  • Organizer: Keystone Symposium on Chemical Senses
  • Place of Presentation: Utah, USA
  • Year and Date: 20070121-20070124
• [Book] Encyclopedia of Neuroscience Vol.7. (2009)
  • Author(s): Touhara, K.
  • Total Pages: 163-169
  • Publisher: Oxford, Academic Press
• [Book] Handbook of Neurochemistry and Molecular Neurobiology Vol.1 Signaling in Neural Development and Function (2009)
  • Author(s): Touhara, K.
  • Total Pages: 141-162
  • Publisher: Springer
• [Book] The Senses : a comprehensive reference, Vol.4, Olfaction & Taste (2008)
  • Author(s): Touhara, K.
  • Total Pages: 527-544
  • Publisher: San Diego, Academic Press
• [Book] 香りを感知する嗅覚のメカニズム (2007)
  • Author(s): 東原和成
  • Total Pages: 66
  • Publisher: 八十八出版
• [Book] G protein coupled receptors : structure, function, and ligand screening (2006)
  • Author(s): Touhara, K., Katada, S., Nakagawa, T., Oka, Y.
  • Total Pages: 85-109
  • Publisher: CRC
• [Remarks] ホームページ等
  • URL: http://park.itc.u-tokyo.ac.jp/biological-chemistry/
• [Patent(Industrial Property Rights)] マウスフェロモン (2009)
  • Inventor(s): 東原和成, 菊水健史, 寺沢宏明
  • Industrial Property Rights Holder: 東京大学、麻布大学、熊本大学
  • Industrial Property Number: 特願,2009-211748
  • Filing Date: 2009-09-14