Dynamic Multi-photon Spectroscopic Imaging of Intracellular Biomolecules Using a White-light Laser Source

2011 Fiscal Year Final Research Report

• Project Area: Molecular Science for Supra Functional Systems ? Development of Advanced Methods for Exploring Elementary Process
• Project/Area Number: 19056010
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: University of Tsukuba (2011); The University of Tokyo (2007-2010)
• Principal Investigator: KANO Hideaki 筑波大学, 数理物質系, 准教授 (70334240)
• Project Period (FY): 2007 – 2011
• Keywords: 分子分光学 / 分光イメージング / 生細胞

Research Abstract

In the present project, we extended our CARS work further to obtain CARS and other multi-photon processes simultaneously. Owing to the ultrabroadband spectral profile of the white-light laser source, we developed label-free tetra-modal molecular imaging system for the SH (second harmonics), TH (third harmonics), multiplex TSF and multiplex CARS processes, and applied this technique to a living cell.

Research Products

• [Journal Article] Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation) (2012)
  • Author(s): Hiroki Segawa, Masanari Okuno, Hideaki Kano, Phileppe Leproux, Vincent Couderc, and Hiro-o Hamaguchi
  • Journal Title: Opt. Express Volume: 20 Pages: 9551-9557
  • DOI: DOI:10.1364/OE.20.009551
  • Peer Reviewed
• [Journal Article] Protein Secondary Structure Imaging with Ultrabroadband Mutiplex Coherent Anti-Stokes Raman Scattering (CARS) Microspectroscopy (2012)
  • Author(s): Kotatsu Bito, Masanari Okuno, Hideaki Kano, Shihomi Tokuhara, Satoru Naito, Yoshinori Masukawa, Philippe Leproux, Vincent Couderc, and Hiro-o Hamaguchi
  • Journal Title: J. Phys. Chem. B Volume: 116 Pages: 1452-1457
  • DOI: DOI:10.1021/jp210914x
  • Peer Reviewed
• [Journal Article] New opportunities offered by compact sub-nanosecond supercontinuum sources in ultra-broadbandmultiplex CARS microspectroscopy (2011)
  • Author(s): Philippe Leproux, Vincent Couderc, A. de Angelis, Masanari Okuno, Hideaki Kano, and Hiro-o Hamaguchi
  • Journal Title: J. Raman Spectrosc. Volume: 42 Pages: 1871-1874
  • DOI: DOI:10.1002/jrs.2995
  • Peer Reviewed
• [Journal Article] Quantitative Coherent Anti-Stokes Raman Scattering (CARS) Microscopy (2011)
  • Author(s): James P. R. Day, Katrin F. Domke, Gianluca Rago, Hideaki Kano, Hiro-o Hamaguchi, Erik M. Vartiainen, and Mischa Bonn
  • Journal Title: J. Phys. Chem. B Volume: 115 Pages: 7713-7725
  • DOI: DOI:10.1021/jp200606e
  • Peer Reviewed
• [Journal Article] 白色レーザーを用いた生細胞イメージング (2011)
  • Author(s): 加納英明、奥野将成、濱口宏夫
  • Journal Title: 表面科学 Volume: 32 Pages: 792-796
  • DOI: DOI:10.1380/jsssj.32.792
• [Journal Article] Quantitative CARS molecular fingerprinting of single living cells with the use of the maximum entropy method (2010)
  • Author(s): Masanari Okuno, Hideaki Kano, Philippe Leproux, Vincent Couderc, James Day, Mischa Bonn, and Hiro-o Hamaguchi
  • Journal Title: Angew. Chem. Int. Ed. Volume: 49 Pages: 6773-6777
  • DOI: DOI:10.1002/anie.201001560
  • Peer Reviewed
• [Journal Article] Molecular Spectroscopic Imaging Using a White-Light Laser Source (2010)
  • Author(s): Hideaki Kano
  • Journal Title: Bull. Chem. Soc. Jpn Volume: 83 Pages: 735-743
  • DOI: DOI:10.1246/bcsj.20100004
  • Peer Reviewed
• [Journal Article] 脂質分子を"ありのまま"にとらえる-白色レーザーを用いた脂質分子のCARS 分光イメージング- (2010)
  • Author(s): 加納英明、奥野将成、濱口宏夫
  • Journal Title: 実験医学 Volume: 28(8) Pages: 1234-1240
  • URL: http://www.yodosha.co.jp/jikkenigaku/book/9784758100595/
• [Journal Article] ナノ秒白色レーザーを用いたコヒーレント・ラマン分光イメージング (2009)
  • Author(s): 加納英明、奥野将成、濱口宏夫
  • Journal Title: レーザー研究 Volume: 37(10) Pages: 739-745
  • URL: http://ci.nii.ac.jp/naid/10025517333
• [Journal Article] コヒーレントラマン分光イメージング (2009)
  • Author(s): 加納英明、濱口宏夫
  • Journal Title: オプトロニクス Volume: 8月号(332) Pages: 108-114
  • URL: http://ci.nii.ac.jp/naid/40016736353
• [Journal Article] 生細胞のラマン分光イメージング ～生命の分子レベル時空間解析に向けて～ (2009)
  • Author(s): 奥野将成、加納英明、濱口宏夫
  • Journal Title: 遺伝 Volume: 63(3) Pages: 80-84
  • URL: http://jglobal.jst.go.jp/public/20090422/200902290299217302
• [Journal Article] Molecular Vibrational Imaging of a Human Cell by Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy Using a Supercontinuum Light Source (2008)
  • Author(s): Hideaki Kano
  • Journal Title: J. Raman Spectrosc. Volume: 39(11) Pages: 1649-1652
  • DOI: DOI:10.1002/jrs.2041
  • Peer Reviewed
• [Journal Article] Intensity enhancement and selective detection of proximate solvent molecules by molecular near-field effect in resonance hyper-Raman scattering (2008)
  • Author(s): Rintaro Shimada, Hideaki Kano, and Hiro-o Hamaguchi, and Hiro-o Hamaguchi
  • Journal Title: J. Chem. Phys. Volume: 129 Pages: 024505
  • DOI: DOI:10.1063/1.2950092
  • Peer Reviewed
• [Journal Article] Ultrabroadband multiplex CARS microspectroscopy and imaging using a subnanosecond supercontinuum light source in the deep near infrared (2008)
  • Author(s): Masanari Okuno, Hideaki Kano, Philippe Leproux, Vincent Couderc, and Hiro-o Hamaguchi
  • Journal Title: Opt. Lett. Volume: 33 Pages: 923-925
  • DOI: DOI:10.1364/OL.33.000923
  • Peer Reviewed
• [Journal Article] 白色レーザーを用いた非線形ラマン分光イメージング (2008)
  • Author(s): 加納英明
  • Journal Title: 分光研究 Volume: 57(5) Pages: 215-217
  • URL: http://ci.nii.ac.jp/naid/10021874433
• [Journal Article] 非線形ラマン分光イメージング (2008)
  • Author(s): 加納英明, 濱口宏夫(章著)
  • Journal Title: ぶんせき Volume: 402 Pages: 270-277
  • URL: http://ci.nii.ac.jp/naid/10025214291
• [Journal Article] Coherent Raman Imaging of Human Living Cells Using a Supercontinuum Light Source (2007)
  • Author(s): Hideaki Kano and Hiro-o Hamaguchi
  • Journal Title: Jpn. J. Appl. Phys. Volume: 46 Pages: 6875-6877
  • DOI: DOI:10.1143/JJAP.46.6875
  • Peer Reviewed
• [Journal Article] Supercontinuum Dynamically Visualizes a Dividing Single Cell (2007)
  • Author(s): Hideaki Kano and Hiro-o Hamaguchi
  • Journal Title: Anal. Chem. Volume: 79 Pages: 8967-8973
  • DOI: DOI:10.1021/ac071416z
  • Peer Reviewed
• [Journal Article] Ultrabroadband (>2000 cm^<-1>) Multiplex Coherent Anti-Stokes Raman Scattering Spectroscopy Using a Sub-Nanosecond Supercontinuum Light Source (2007)
  • Author(s): Masanari Okuno, Hideaki Kano, Philippe Leproux, Vincent Couderc, and Hiro-o Hamaguchi
  • Journal Title: Opt. Lett. Volume: 32 Pages: 3050-3052
  • DOI: DOI:10.1364/OL.32.003050
  • Peer Reviewed
• [Presentation] CARS molecular fingerprinting using a white-light laser source (2010)
  • Author(s): Hideaki Kano, Masanari Okuno, and Hiro-o Hamaguchi
  • Organizer: The 22nd International Conference on Raman Spectroscopy
  • Place of Presentation: Boston, U.S.A.
  • Year and Date: 20100808-13
• [Presentation] Label-free, multi-colour, high-speed imaging of a living cell by CARS spectral imaging (2010)
  • Author(s): Hideaki Kano, Masanari Okuno, and Hiro-o Hamaguchi
  • Organizer: Laser Application in Life Sciences Conference (LALS-2010)
  • Place of Presentation: Oulu, Finland
  • Year and Date: 20100609-11
• [Presentation] CARS spectroscopic imaging using a white-light laser source (2009)
  • Author(s): Hideaki Kano
  • Organizer: 5th International Conference on Advanced Vibrational Spectroscopy (ICAVS-5)
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20090712-17
• [Presentation] CARS Spectroscopic Imaging Using a Supercontinuum Laser Source (2008)
  • Author(s): Hideaki Kano and Hiro-o Hamaguchi
  • Organizer: International Conference on Raman Spectroscopy (ICORS 2008)
  • Place of Presentation: Uxbridge, UK
  • Year and Date: 20080817-22
• [Presentation] COHERENT RAMAN BIOIMAGING USING A SUPERCONTINUUM LIGHT SOURCE (2007)
  • Author(s): Hideaki Kano and Hiro-o Hamaguchi
  • Organizer: International Symposium, TOPICAL PROBLEMS OF BIOPHOTONICS 2007
  • Place of Presentation: Moscow, Russia
  • Year and Date: 20070804-11
• [Book] Linear and Non-Linear Raman Microspectroscopy -from a Molecule to Single Living Cells- Raman, Infrared, and Near-infrared chemical imaging, S. Sasic and Y. Ozaki Ed. Eds. (2010)
  • Author(s): Hideaki Kano, Yu-San Huang, Yasuaki Naito, Rintaro Shimada, and Hiro-o Hamaguchi(章著)
  • Total Pages: 301-312
  • Publisher: Wiley
  • URL: http://as.wiley.com/WileyCDA/WileyTitle/productCd-047038204X.html
• [Book] Linear and Non-Linear Raman Microspectroscopy and Imaging of Single Living Cells Nao Biophotonics, Hiroshi Masuhara, Satoshi Kawata, Fumio Tokunaga eds. (2007)
  • Author(s): Hideaki Kano, Yu-San Huang, Yasuaki Naito, and Hiro-o Hamaguchi(章著)
  • Total Pages: 43-56
  • Publisher: Elsevier
  • URL: http://www.sciencedirect.com/science/bookseries/15740641
• [Book] ラマン顕微分光法 ～生細胞を分子レベルで観る～実験医学別冊,生命科学のための機器分析実験ハンドブック (2007)
  • Author(s): 安藤正浩, 内藤康彰, 加納英明, 濱口宏夫(章著)
  • Total Pages: 74-79
  • URL: http://www.yodosha.co.jp/jikkenigaku/book/9784758101691/
• [Patent(Industrial Property Rights)] 非線形分光計測システム用の光源装置,非線形分光計測システム及び方法 (2008)
  • Inventor(s): 加納英明・濱口 宏夫
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 特願2008-66832
  • Filing Date: 2008-03-14