Elucidation of structural basis of dynamically-driven protein allostery by time-resolved resonance Raman spectroscopy

2011 Fiscal Year Final Research Report

• Project Area: Molecular Science for Supra Functional Systems ? Development of Advanced Methods for Exploring Elementary Process
• Project/Area Number: 19056013
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Osaka University
• Principal Investigator: MIZUTANI Yasuhisa 大阪大学, 大学院・理学研究科, 教授 (60270469)
• Co-Investigator(Kenkyū-buntansha): ISHIKAWA Haruto 大阪大学, 大学院・理学研究科, 講師 (40551338) ; MIZUNO Misao 大阪大学, 大学院・理学研究科, 助教 (10464257)
• Project Period (FY): 2007 – 2011
• Keywords: ラマン分光法 / 生物物理学 / 時間分解分光学

Research Abstract

Protein dynamics highly relevant to biological function were studied with time-resolved resonance Raman spectroscopy. Our effort on developments on the measurement system enabled us to perform site-specific observation on structural changes of proteins in wide time range from picoseconds to milliseconds. We elucidated functionally-important dynamics of various allosteric proteins such as hemoglobins, heme-based gas sensors, and photoactive proteins with the high performance of the system.

Research Products

• [Journal Article] Differences between Protein Dynamics of Hemoglobin upon Dissociation of Oxygen and Carbon Monoxide (2012)
  • Author(s): Yuka Murakawa, Masako Nagai and Yasuhisa Mizutani
  • Journal Title: J. Am. Chem. Soc. Volume: 134 Pages: 1434-1437
  • DOI: DOI:10.1021/ja209659w
  • Peer Reviewed
• [Journal Article] Protein Dynamics of Isolated Chains of Recombinant Human Hemoglobin Elucidated by Time-resolved Resonance Raman Spectroscopy (2012)
  • Author(s): Kenta Yamada, Haruto Ishikawa, and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. B Volume: 116 Pages: 1992-1998
  • DOI: DOI:10.1021/jp2114303
  • Peer Reviewed
• [Journal Article] Direct observation of the structural change of Tyr174 in the primary reaction of sensory rhodopsin II (2011)
  • Author(s): Misao Mizuno, Yuki Sudo, Michio Homma and Yasuhisa Mizutani
  • Journal Title: Biochemistry Volume: 50 Pages: 3170-3180
  • DOI: DOI:10.1021/bi101817y
  • Peer Reviewed
• [Journal Article] Changes in the Hydrogen-Bond Network around the Chromophore of Photoactive Yellow Protein in the Ground and Excited States (2011)
  • Author(s): Misao Mizuno, Hironari Kamikubo, Mikio Kataoka and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. B Volume: 115 Pages: 9306-9310
  • DOI: DOI:10.1021/jp2029399
  • Peer Reviewed
• [Journal Article] Direct observation of vibrational energy flow in cytochrome c (2011)
  • Author(s): Naoki Fujii, Misao Mizuno, and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. B Volume: 115 Pages: 13057-13064
  • DOI: DOI:10.1021/jp207500b
  • Peer Reviewed
• [Journal Article] Protein Conformational Changes of the Oxidative-Stress Sensor, SoxR, upon Redox Change of the [2Fe-2S] Cluster Probed with Ultraviolet Resonance Raman Spectroscopy (2011)
  • Author(s): Kazuo Kobayashi, Misao Mizuno, and Yasuhisa Mizutani
  • Journal Title: Biochemistry Volume: 50 Pages: 9468-9474
  • DOI: DOI:10.1021/bi201526y
  • Peer Reviewed
• [Journal Article] Picosecond Time-Resolved Ultraviolet Resonance Raman Spectroscopy of Bacteriorhodopsin: Primary Protein Response to the Photoisomerization of Retinal (2009)
  • Author(s): Misao Mizuno, Mikihiro Shibata, Junya Yamada, Hideki Kandori, and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. B Volume: 113 Pages: 12121-12128
  • DOI: DOI:10.1021/jp904388w
  • Peer Reviewed
• [Journal Article] Photoinduced Electron Transfer in Glucose Oxidase: a Picosecond Time-resolved Ultraviolet Resonance Raman Study (2008)
  • Author(s): Akiko Fujiwara and Yasuhisa Mizutani
  • Journal Title: J. Raman Spectrosc. Volume: 39 Pages: 1600-1605
  • DOI: DOI:10.1002/jrs.2077
  • Peer Reviewed
• [Journal Article] Resonance Raman Observation of the Structural Dynamics of FixL on Ligand Recognition and Signaling (2007)
  • Author(s): Yusuke Hiruma, Akihiro Kikuchi, Atsunari Tanaka, Yoshitsugu Shiro, and Yasuhisa Mizutani
  • Journal Title: Biochemistry Volume: 46 Pages: 6086-6096
  • DOI: DOI:10.1021/bi062083n
  • Peer Reviewed
• [Journal Article] Primary protein response after ligand photodissociation in carbonmonoxy myoglobin (2007)
  • Author(s): Akira Sato, Ying Gao, Teizo Kitagawa, and Yasuhisa Mizutani
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 104 Pages: 9627-9632
  • DOI: DOI:10.1073/pnas.0611560104
  • Peer Reviewed
• [Presentation] Watching energy flow in hemeproteins (2011)
  • Author(s): Yasuhisa Mizutani
  • Organizer: Telluride Science Research Center Workshop on Protein Dynamics
  • Place of Presentation: Telluride, Colorado, USA.(招待講演)
  • Year and Date: 20110801-05
• [Presentation] Protein dynamics of photosensory proteins as studied by time-resolved resonance Raman spectroscopy (2011)
  • Author(s): Yasuhisa Mizutani
  • Organizer: The 5th Asia and Oceania Conference for Photobiology
  • Place of Presentation: 奈良県立新公会堂(招待講演)
  • Year and Date: 20110730-0801
• [Presentation] Elucidation of ultrafast protein dynamics by picosecond time-resolved visible and ultraviolet resonance Raman spectroscopy (2010)
  • Author(s): Yasuhia Mizutani
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, Hawaii, USA.(招待講演)
  • Year and Date: 20101215-20
• [Presentation] Protein dynamics of sensory proteins as studied by time-resolved resonance Raman spectroscopy (2010)
  • Author(s): Yasuhia Mizutani
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, Hawaii, USA.(招待講演)
  • Year and Date: 20101215-20
• [Presentation] Primary Protein Responses to Chromophore Isomerization of Photosensary Proteins (2010)
  • Author(s): Yasuhisa Mizutani
  • Organizer: 22nd International Conference on Raman Spectroscopy (ICORS 2010)
  • Place of Presentation: Boston, USA.(招待講演)
  • Year and Date: 20100808-13
• [Presentation] Primary protein responses to chromophore isomerization: picosecond time-resolved resonance Raman studies (2010)
  • Author(s): Yasuhisa Mizutani
  • Organizer: The 6th Asian Conference on Ultrafast Phenomena
  • Place of Presentation: Taipei.(招待講演)
  • Year and Date: 20100110-13
• [Presentation] Ultrafast protein response to photodissociation and photoisomerization: Myoglobin and bacteriorhodopsin (2009)
  • Author(s): Yasuhisa Mizutani
  • Organizer: 3e cycle lecture tour in Switzerland: Protein dynamics and function
  • Place of Presentation: Universitat Basel(招待講演)
  • Year and Date: 2009-10-14
• [Presentation] Hemoglobin allostery: dynamics and function (2008)
  • Author(s): Yasuhisa Mizutani
  • Organizer: 28th Annual Pittsburgh Conference Lectures
  • Place of Presentation: University of Pittsburgh, USA.(招待講演)
  • Year and Date: 2008-04-16
• [Book] みず学への誘い, 88-97 (2008)
  • Author(s): 水谷泰久
  • Total Pages: 300
  • Publisher: 大阪大学出版社
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html