Mechanism of fertilization and epigenomic regulation of germ cells.

2010 Fiscal Year Self-evaluation Report

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062008
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: OKABE Masaru Osaka University, 微生物病研究所, 教授 (30089875)
• Project Period (FY): 2008 – 2012
• Keywords: 生殖細胞 / 遺伝子組換え動物 / 不妊 / 異種キメラ

Research Abstract

生殖系列細胞は、精子と卵子へ分化・成熟し、受精していったん消滅するが、胚から個体へと発生していく過程で新たに誕生する。この新たな生殖系列細胞も生理的、エピジェネティック的に受精能を有し個体として発生できる「機能する配偶子」へと分化していく。本研究では、この連綿と続く生殖系列の世代サイクルを分子生物学的に明らかにするために、受精現象とその前後のエピゲノム調節に焦点を当て、試験管内では再現できない現象について遺伝子操作動物をもちい解き明かす。

Research Products

• [Journal Article] Formation of a thymus from rat ES cells in xenogeneic nude mouse-rat ES chimeras. (2011)
  • Author(s): Isotani A, Hatayama H, Kaseda K, Ikawa M, Okabe M.
  • Journal Title: Genes to Cells 16(4) Pages: 397-405
  • Peer Reviewed
• [Journal Article] Calsperin is a testis specific chaperone required for sperm fertility. (2011)
  • Author(s): Ikawa M, Tokuhiro K, Yamaguchi R, Benham AM, Tamura T, Wada I, Satouh Y, Inoue N, Okabe M.
  • Journal Title: J Biol Chem 18;286(7) Pages: 5639-5646
  • Peer Reviewed
• [Journal Article] From the Cover : Pravastatin induces placental growth factor(PGF)and ameliorates preeclampsia in a mouse model. (2011)
  • Author(s): Kumasawa K, Ikawa M, Kidoya H, Hasuwa H, Saito-Fujita T, Morioka Y, Takakura N, Kimura T, Okabe M.
  • Journal Title: Proc Natl Acad Sci USA 108 Pages: 1451-1455
  • Peer Reviewed
• [Journal Article] Identification and disruption of sperm-specific angiotensin converting enzyme-3(ACE3)in mouse. (2010)
  • Author(s): Inoue N, Kasahara T, Ikawa M, Okabe M.
  • Journal Title: PLoS ONE 5 Pages: e10301
  • Peer Reviewed
• [Journal Article] Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse. (2010)
  • Author(s): Fujihara Y, Murakami M, Inoue N, Satouh Y, Kaseda K, Ikawa M, Okabe M.
  • Journal Title: J Cell Sci. 1;123(Pt 9) Pages: 1531-1536
  • Peer Reviewed
• [Presentation] The Gene-Manipulated Animals and Research in Mechanism of Fertilization (2010)
  • Author(s): Masaru Okabe
  • Organizer: 4th AFLAS Congress Academic Committee
  • Place of Presentation: Taipei International Convention Center,中華民国
  • Year and Date: 2010-11-09
• [Presentation] Fertilization in vivo and Fertilization in vitro (2010)
  • Author(s): Masaru Okabe
  • Organizer: International Symposium for Immunology of Reproduction joint meeting in conjunction with The 25th Annual Meeting of Japan Society for Immunology of Reproduction
  • Place of Presentation: Osaka University
  • Year and Date: 2010-08-28
• [Presentation] Regulation of sperm migration into the oviduct (2010)
  • Author(s): Masaru Okabe
  • Organizer: 2010 Reproductive Tract Biology Gordon Research Conference
  • Place of Presentation: Proctor Academy, USA
  • Year and Date: 2010-08-15
• [Presentation] 遺伝子改変動物を通して見る精子と卵子の出会い (2010)
  • Author(s): 岡部勝
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都市南区京都テルサ(招待講演)
  • Year and Date: 2010-05-13
• [Presentation] Mechanisms of Fertilization-A View Through Gene Manipulated Mice (2010)
  • Author(s): Masaru Okabe
  • Organizer: American Society of Andrology ASA 35th AnnualMeeting
  • Place of Presentation: Houston, TX Omni Houston Hotel(USA)(招待講演)
  • Year and Date: 2010-04-13
• [Remarks] ホームページ
  • URL: http://kumikae01.gen-info.osaka-u.ac.jp/tg/greenmouse.cfm