2012 Fiscal Year Final Research Report
Mechanism of mammalian fertilization and epigenomic regulation of germline cells.
| Project Area | The germline: its developmental cycle and epigenome network |
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| Project/Area Number |
20062008
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Osaka University |
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Principal Investigator |
OKABE Masaru 大阪大学, 微生物病研究所, 教授 (30089875)
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| Co-Investigator(Kenkyū-buntansha) |
ISOTANI Ayako 大阪大学, 微生物病研究所, 特任准教授 (20444523)
HASUWA Hidetoshi 大阪大学, 微生物病研究所, 助教 (50343249)
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| Project Period (FY) |
2008 – 2012
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| Keywords | マイクロRNA / エピジェネティック / ノックアウトマウス / 生殖細胞 / 遺伝子組換え動物 / 不妊 / 分子シャペロン / 受精 |
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| Research Abstract |
Spermatozoa must have fertilizing ability other than to have sperm specific shape and moving ability. We clarified that Spesp1, Calsperin, Pdilt and Tex101 are all essentially required for spermatozoa to establish their fertilizing ability by making gene-manipulated animals. We also revealed the precise mechanism of sperm dynamics in female reproductive tract using these gene-manipulated animals and live-imaging systems. Furthermore, miR-200b and miR-429 were shown to be essential for female ovulation by gene-disruption experiments.
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[Journal Article]2012
Author(s)
Yamaguchi R, Fujihara Y, Ikawa M, Okabe M.Tokuhiro K, Ikawa M, Benham AM, Okabe M
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Journal Title
Proc Natl Acad Sci U S A
Volume: 109
Pages: 3850-5
DOI
Peer Reviewed
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