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2009 Fiscal Year Annual Research Report

分光学的手法による生体π空間の制御機構解明と新機能の開拓

Planned Research

Project AreaEmergence of highly elaborated pai-space and its function
Project/Area Number 20108014
Research InstitutionNagoya Institute of Technology

Principal Investigator

神取 秀樹  Nagoya Institute of Technology, 工学研究科, 教授 (70202033)

Keywordsπ空間 / 生体分子 / 発色団 / 赤外分光 / アミノ酸変異
Research Abstract

生体分子は進化の中で最適化されたπ空間の制御系であり、蛋白質などのナノ反応場が精緻な制御を可能にしている。とりわけ光受容蛋白質は、レチナール、クロロフィル、フラビンといった分子のπ電子系を制御することでユニークな色や反応、機能を産み出しており、ボトムアップ型の高次π空間を創製するにあたってのゴールともみなすことができる。本研究では、蛋白質による特異な波長制御、反応制御、機能制御に着目し、遺伝子改変によって変異を導入した光受容蛋白質に対して、赤外分光法などの分光学的手法を駆使した実験研究によりメカニズム解明を目指している。本年度は以下の成果を得ることができた。
ロドプシンの生体π空間制御では、これまでに我々のグループで発見した色変化をもたらすプロテオロドプシンのループ領域のアミノ酸変異を分光学的手法により解析した結果、変異がプロテオロドプシンの部位特異的であることを明らかにした。また、クロライドを結合するハロロドプシンとフォボロドプシンについて、赤外分光を用いた詳細な構造解析により、結合の特異性を明らかにすることができた。さらに新たに発見された光センサー機能をもつロドプシンの詳細な構造解析を行った。
光合成反応中心の生体π空間制御では、蛋白質内部に結合した水分子の信号を帰属することに成功した。フラビン蛋白質の生体π空間制御では、Jαヘリックスの役割を変異蛋白質を用いた構造解析により明らかにするとともに、その構造変化が生物種によって異なることを見出した。
これらの成果も含め、11編の原著論文を世に出すことができたが、これらはいずれも生体π空間の特異性に迫る実験研究と位置付けることができる。

  • Research Products

    (54 results)

All 2009

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (43 results)

  • [Journal Article] Engineering an inward proton transport from a bacterial sensor rhodopsin2009

    • Author(s)
      A.Kawanabe, Y.Furutani, K.-H.Jung, H.Kandori
    • Journal Title

      J.Am.Chem.Soc. 131

      Pages: 16439-16444

    • Peer Reviewed
  • [Journal Article] Interaction between Na+ Ion and Carboxylates of the PomA-PomB Stator Unit Studied by ATR-FTIR Spectroscopy2009

    • Author(s)
      Y.Sudo, Y.Kitade, Y.Furutani, M.Kojima, S.Kojima, M.Homma, H.Kandori
    • Journal Title

      Biochemistry 48

      Pages: 11699-11705

    • Peer Reviewed
  • [Journal Article] Characterization of a Signaling Complex Composed of Sensory Rhodopsin I and Its Cognate Transducer Protein from the Eubacterium Salinibacter ruber2009

    • Author(s)
      Y.Sudo, A.Okada, D.Suzuki, K, Inoue, H.Irieda, M.Sakai, M.Fujii, Y.Furutani, H.Kandori, M.Homma
    • Journal Title

      Biochemistry 48

      Pages: 10136-10145

    • Peer Reviewed
  • [Journal Article] Picosecond time-resolved ultrafast resonance Raman spectroscopy of bacteriorhodopsin : Primary protein response to the photoisomerization of retinal2009

    • Author(s)
      M.Mizuno, M.Shibata, J.Yamada, H.Kandori, Y.Mizutani
    • Journal Title

      J.Phys.Chem.B 113

      Pages: 12121-12128

    • Peer Reviewed
  • [Journal Article] Different Role of the Jα Helix in the Light-Induced Activation of the LOV2 Domains in Various Phototropins2009

    • Author(s)
      T.Koyama, T.Iwata, A.Yamamoto, Y.Sato, D.Matsuoka, S.Tokutomi, H.Kandori
    • Journal Title

      Biochemistry 48

      Pages: 7621-7628

    • Peer Reviewed
  • [Journal Article] Effect of Chloride Ion Binding on the Photochemical Properties of Salinibacter Sensory Rhodopsin I2009

    • Author(s)
      D.Suzuki, Y.Furutani, K.Inoue, T.Kikukawa, M.Sakai, M.Fujii, H.Kandori.M.Homma, Y.Sudo
    • Journal Title

      J.Mol.Biol. 392

      Pages: 48-62

    • Peer Reviewed
  • [Journal Article] Manipulation of Protein-Complex Function by Using an Engineered Heterotrimeric Coiled-Coil Switch2009

    • Author(s)
      T.Mizuno, K.Suzuki, T.Imai, Y.Kitade, Y.Furutani, M.Kudou, M.Oda, H.Kandori, K.Tsumoto, T.Tanaka
    • Journal Title

      Org.Biomol.Chem. 7

      Pages: 3102-3111

    • Peer Reviewed
  • [Journal Article] Property of Anion Binding Site of pharaonis Halorhodopsin Studied by Ultrafast Pump-Probe Spectroscopy and Low-Temperature FTIR Spectroscopy2009

    • Author(s)
      K.Nakashima, T.Nakamura, S.Takeuchi, M.Shibata, M.Demura, T.Tahara, H.Kandori
    • Journal Title

      J.Phys.Chem.B 113

      Pages: 8429-8434

    • Peer Reviewed
  • [Journal Article] Color-Changing Mutation in the E-F Loop of Propeorhodopsin2009

    • Author(s)
      M.Yoshitsugu, J.Yamada, H.Kandori
    • Journal Title

      Biochemistry 48

      Pages: 4324-4330

    • Peer Reviewed
  • [Journal Article] Spectroscopic and Kinetic Evidence on How Bacteriorhodopsin Accomplishes Vectorial Proton Transport under Functional Conditions2009

    • Author(s)
      V.A.Lorenz-Fonfria, H.Kandori
    • Journal Title

      J.Am.Chem.Soc. 131

      Pages: 5891-5901

    • Peer Reviewed
  • [Journal Article] Photoreactions and Structural Changes of Anabaena Sensory Rhodopsin2009

    • Author(s)
      A.Kawanabe, H.Kandori
    • Journal Title

      Sensors 9

      Pages: 9544-9607

    • Peer Reviewed
  • [Presentation] Bio-π-space in photoreceptive proteins : Spectroscopic investigation and creation of new functions2009

    • Author(s)
      H.Kandori
    • Organizer
      International Symposium on Highly Elaborated-Space and Its Function
    • Place of Presentation
      Osaka
    • Year and Date
      2009-12-18
  • [Presentation] FTIR study of metal-replaced bacterial photosynthetic reaction center2009

    • Author(s)
      A.Watanabe, T.Iwata, MY.Okamura, ML.Paddock, H.Kandori
    • Organizer
      International Symposium on Highly Elaborated-Space and Its Function
    • Place of Presentation
      Osaka
    • Year and Date
      2009-12-18
  • [Presentation] Study on chimeric archeal-rhodopsin containing cytoplasmic loop of bovine rhodopsin2009

    • Author(s)
      A.Nakatsuma, T.Yamashita, A.Kawanabe, Y.Furutani, Y.Shichida, H.Kandori
    • Organizer
      International Symposium on Highly Elaborated-Space and Its Function
    • Place of Presentation
      Osaka
    • Year and Date
      2009-12-18
  • [Presentation] Mechanism of color-tuning mutation in the E-F loop of proteorhodopsin2009

    • Author(s)
      K.Yamada, A.Kawanabe, M.Yoshitsugu, H.Kandori
    • Organizer
      International Symposium on Highly Elaborated-Space and Its Function
    • Place of Presentation
      Osaka
    • Year and Date
      2009-12-18
  • [Presentation] 低温および時間分解赤外分光法によるロドプシンの構造変化の研究2009

    • Author(s)
      神取秀樹
    • Organizer
      特定領域研究「高次系分子科学」第4回公開シンポジウム
    • Place of Presentation
      別府
    • Year and Date
      2009-11-26
  • [Presentation] フラビンを発色団とする光センサータンパク質の反応制御機構の解明2009

    • Author(s)
      岩田達也、神取秀樹
    • Organizer
      高次π空間の創発と機能開発第1回若手研究会
    • Place of Presentation
      仙台
    • Year and Date
      2009-11-19
  • [Presentation] Bio-π-space in photoreceptive proteins2009

    • Author(s)
      H.Kandori
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-31
  • [Presentation] FTIR study of two BLUF domains, AppA and PAC2009

    • Author(s)
      T.Iwata, A.Watanabe, M.Iseki, M.Watanabe, H.Kandori
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-31
  • [Presentation] The mutation study to create proton pumps from a bacterial sensor rhodopsin2009

    • Author(s)
      A.Kawanabe, K.H.Jung, H.Kandori
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-31
  • [Presentation] FTIR Spectroscopy Directly Monitors Photoactivation and Photorepairing Processes of the (6-4) Photolyase2009

    • Author(s)
      Y.Zhang, T.Iwata, K.Hitomi, J.Yamamoto, S.Iwai, T.Todo, E.D.Getzoff H.Kandori
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-31
  • [Presentation] Photoreaction of iLOV, an FMN-binding fluorescence protein2009

    • Author(s)
      N.Hozumi, Y.Zhang, T.Iwata, K.Hitomi, E.D.Getzoff, J.M.Christie, H.Kandori
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-31
  • [Presentation] Encapsulation of ion-pumping rhodopsins into multi-wall carbon nanotubes2009

    • Author(s)
      T.Kawashima, K.Yanagi, Z.Liu, Y.Furutani, K.Suenaga, H.Kataura, H.Kandori
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-31
  • [Presentation] Study on chimeric archeal-rhodopsin containing cytoplasmic loop of bovine rhodopsin2009

    • Author(s)
      A.Nakatsuma, T.Yamashita, A.Kawanabe, Y.Furutani, Y.Shichida, H.Kandori
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-31
  • [Presentation] Spectroscopic study of proton release group of archaerhodopsins2009

    • Author(s)
      J.Yamada, K.Ihara, H.Kandori
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-31
  • [Presentation] FTIR study of metal-replaced bacterial photosynthetic reaction center2009

    • Author(s)
      A.Watanabe, T.Iwata, M.L.Paddock, M.Y.Okarnura, H.Kandori
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-31
  • [Presentation] ATR-FTIR study of KcsA channel2009

    • Author(s)
      Y.Asai, Y.Furutani, H.Shimizu, S.Oiki, H.Kandori
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-31
  • [Presentation] An FTIR Study of Monkey Green-and Red-Sensitive Visual Pigments2009

    • Author(s)
      K.Katayama, Y.Furutani, H.Imai, H.Kandori
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-31
  • [Presentation] Color-Tuning Position in the E-F loop of Proteorhodopsin2009

    • Author(s)
      K.Yamada, A.Kawanabe, M.Yoshitsugu, H.Kandori
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-31
  • [Presentation] Strongly Hydrogen-Bonded Water Molecule as the Determinant of Proton-Pump Function of Rhodopsins2009

    • Author(s)
      H.Kandori
    • Organizer
      Horiba-ISSP International Symposium (ISSP-11) on "Hydrogen and Water in Condensed Matter Physics"
    • Place of Presentation
      Chiba
    • Year and Date
      2009-10-13
  • [Presentation] Identification of FTIR Bands Due to Internal Water Molecules around the Quinone Binding Sites in the Bacterial Reaction Center2009

    • Author(s)
      T.Iwata, M.L.Paddock, M.Y.Okamura, H.Kandori
    • Organizer
      Horiba-ISSP International Symposium (ISSP-11) on "Hydrogen and Water in Condensed Matter Physics"
    • Place of Presentation
      Chiba
    • Year and Date
      2009-10-13
  • [Presentation] Spectroscopic Study of Proton Release Grpup of Archaerhodopsins2009

    • Author(s)
      J.Yamada, K.Ihara, H.Kandori
    • Organizer
      Horiba-ISSP International Symposium (ISSP-11) on "Hydrogen and Water in Condensed Matter Physics"
    • Place of Presentation
      Chiba
    • Year and Date
      2009-10-13
  • [Presentation] An FTIR Study of Monkey Green-and Red-Sensitive Visual Pigments2009

    • Author(s)
      K.Katayama, Y.Furutani, H.Imai, H.Kandori
    • Organizer
      Horiba-ISSP International Symposium (ISSP-11) on "Hydrogen and Water in Condensed Matter Physics"
    • Place of Presentation
      Chiba
    • Year and Date
      2009-10-13
  • [Presentation] (6-4)光回復酵素によるDNA修復の赤外分光計測2009

    • Author(s)
      張宇、岩田達也、山元淳平、人見研一、岩井成憲、藤堂剛、E.D.Getzoff、神取秀樹
    • Organizer
      第3回分子科学討論会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-24
  • [Presentation] FMN結合型蛍光タンパク質iLovの光反応2009

    • Author(s)
      穂積直樹、岩田達也、張宇、人見研一、E.D.Getzoff、J.M.Christie、神取秀樹
    • Organizer
      第3回分子科学討論会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-24
  • [Presentation] 古細菌型ロドプシンのカーボンナノチューブへの内包2009

    • Author(s)
      川島崇睦、柳和宏、劉崢、古谷祐詞、末永和知、片浦弘道、神取秀樹
    • Organizer
      第3回分子科学討論会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-24
  • [Presentation] 霊長類色覚視物質の赤外分光解析2009

    • Author(s)
      片山耕大、古谷祐詞、今井啓雄、神取秀樹
    • Organizer
      第3回分子科学討論会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-24
  • [Presentation] Light & Life, Light Sensor Protein2009

    • Author(s)
      H.Kandori
    • Organizer
      An International Conference on "Biotechnology for a Sustainable Future"
    • Place of Presentation
      Bali, Indonesia
    • Year and Date
      2009-09-16
  • [Presentation] Structural Analysis of Sodium Ion Binding Sites of V-ATPase from Enterococcus hirae by use of ATR-FTIR Spectroscopy2009

    • Author(s)
      Y.Furutani, T.Murata, H.Kandori
    • Organizer
      Internal Symposium:Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes
    • Place of Presentation
      Kyoto
    • Year and Date
      2009-09-16
  • [Presentation] 1個の水分子が光駆動プロトンポンプ機能を決める?2009

    • Author(s)
      神取秀樹
    • Organizer
      水和ナノ構造研究会
    • Place of Presentation
      北茨城
    • Year and Date
      2009-09-07
  • [Presentation] 光に応答しない膜機能分子の赤外分光測定2009

    • Author(s)
      神取秀樹、古谷祐詞
    • Organizer
      生理学研究所研究会「作動中の膜機能分子の姿を捉える:静止画から動画へ」
    • Place of Presentation
      岡崎
    • Year and Date
      2009-09-03
  • [Presentation] "KcsAチャネルに対する赤外分光の狙い"は達成されたのか?2009

    • Author(s)
      古谷祐詞、神取秀樹
    • Organizer
      特定領域研究「高次系分子科学」第7回ミニ公開シンポジウム
    • Place of Presentation
      越前
    • Year and Date
      2009-08-31
  • [Presentation] 分光学的手法による生体π空間の制御機構解明と新機能の開拓2009

    • Author(s)
      神取秀樹
    • Organizer
      新学術領域研究「高次π空間」第2回公開シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2009-08-28
  • [Presentation] ロドプシンのカーボンナノチューブへの内包2009

    • Author(s)
      川島崇睦、柳和宏、劉崢、古谷祐詞、末永和知、片浦弘道、神取秀樹
    • Organizer
      新学術領域研究「高次π空間」第2回公開シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2009-08-28
  • [Presentation] (6-4)光回復酵素によるDNA修復の赤外分光法を用いた解析2009

    • Author(s)
      張宇、岩田達也、人見研一、山元淳平、岩井成憲、藤堂剛、Elizabeth D.Getzoff、神取秀樹
    • Organizer
      第15回日本光生物学協会年会
    • Place of Presentation
      岡崎
    • Year and Date
      2009-08-19
  • [Presentation] ロドプシンのカーボンナノチューブへの内包2009

    • Author(s)
      川島崇睦、柳和宏、劉崢、古谷祐詞、末永和知、片浦弘道、神取秀樹
    • Organizer
      生物物理若手の会第49回夏の学校
    • Place of Presentation
      支笏湖
    • Year and Date
      2009-08-01
  • [Presentation] 霊長類色覚視物質の赤外分光解析2009

    • Author(s)
      片山耕大、古谷祐詞、今井啓雄、神取秀樹
    • Organizer
      生物物理若手の会第49回夏の学校
    • Place of Presentation
      支笏湖
    • Year and Date
      2009-08-01
  • [Presentation] Proteorhodopsinの色を変えるE-Fループの変異体2009

    • Author(s)
      山田啓介、川鍋陽、吉次麻衣子、神取秀樹
    • Organizer
      生物物理若手の会第49回夏の学校
    • Place of Presentation
      支笏湖
    • Year and Date
      2009-08-01
  • [Presentation] Structural changes accompanying retinal photoisomerization in rhodopsins2009

    • Author(s)
      H.Kandori
    • Organizer
      ICP2009,XXIVInternationalConferenceonPhotochemistry
    • Place of Presentation
      Toledo, Spain
    • Year and Date
      2009-07-21
  • [Presentation] FTIR study of rhodopsins in action2009

    • Author(s)
      H.Kandori
    • Organizer
      15th International Congress on Photobiology : Svmnosium llC "Lieht-Driven Proton Pumns"
    • Place of Presentation
      Dusseldorf, Germany
    • Year and Date
      2009-06-22
  • [Presentation] 全反射赤外分光法によるファラオニスフォボロドプシンの塩化物イオン結合解離に伴う構造変化解析2009

    • Author(s)
      古谷祐詞、北出祐也、加茂直樹、神取秀樹
    • Organizer
      第36回生体分子科学討論会
    • Place of Presentation
      札幌
    • Year and Date
      2009-06-19
  • [Presentation] 古細菌型ロドプシンで実現した内向きのプロトンポンプ2009

    • Author(s)
      川鍋陽、古谷祐詞、Kwang-Hwan Jung、神取秀樹
    • Organizer
      第36回生体分子科学討論会
    • Place of Presentation
      札幌
    • Year and Date
      2009-06-19
  • [Presentation] ATR-FTIR Spectroscopy for Investigating Interaction Changes upon Binding of Ions to Transmembrane Proteins2009

    • Author(s)
      Y.Furutani, H.Kandori
    • Organizer
      First World Congress of International Academy of Nanomedicine (IANM)
    • Place of Presentation
      Sanya, China
    • Year and Date
      2009-06-15
  • [Presentation] Picosecond time-resolved UV resonance Raman spectroscopy of bacteriorhodopsin : Primary protein response to the photoreaction of retinalchromophore2009

    • Author(s)
      M.Mizuno, M.Shibata, J.Yamada, H.Kandori, Y.Mizutani
    • Organizer
      14th International Conference on Time-Resolved Vibrational Spectroscopy
    • Place of Presentation
      Meredith, USA
    • Year and Date
      2009-05-10

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Published: 2011-06-16   Modified: 2016-04-21  

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