2013 Fiscal Year Final Research Report
Reconstruction of Drosophila GSC/niche system in vitro and analysis of GSC differentiation
| Project Area | Regulatory Mechanism of Gamete Stem Cells |
|---|
| Project/Area Number |
20116003
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Biological Sciences
|
|---|
| Research Institution | Ibaraki University |
|---|
Principal Investigator |
NIKI YUZO 茨城大学, 理学部, 教授 (00134164)
|
|---|
| Project Period (FY) |
2008-11-13 – 2013-03-31
|
| Keywords | ショウジョウバエ / 生殖幹細胞 / 細胞培養 / ニッチ / 再構築 / 分化 |
|---|
| Research Abstract |
We succeeded in the reconstitution of GSC/niche system in Drosophila. We established stable cell lines originated from female and male niche cells (cap cells and escort cells in female, and hub and cyst cells in male). Using these cell lines, we identified highly expressed genes in each cell line and found that there are many unique genes specifically expressed in each niche cell line. Furthermore, we found that Drosophila insulin-like genes are also expressed in the niche cells. We constructed co-culture system of GSCs and niche cells and analyzed roles of growth factors. Furthermore, we succeeded in artificial induction of the differentiation and dedifferentiation of GSCs by coculturing with niche cells.
|
