ROS signaling sensing via unique post-translational modification

2010 Fiscal Year Self-evaluation Report

• Project Area: Signaling functions of reactive oxygen species
• Project/Area Number: 20117005
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University (2012-2013); Kumamoto University (2008-2011)
• Principal Investigator: AKAIKE Takaaki Kumamoto University, 大学院・生命科学研究部, 教授 (20231798)
• Project Period (FY): 2008-11-13 – 2013-03-31
• Keywords: 活性酸素 / シグナル伝達 / プロテオーム / 酸化ストレス / 生理活性

Research Abstract

活性酸素シグナル伝達の分子機構は充分理解されていない。活性酸素・NOは、その最も上流に位置する分子群であるが、シグナル伝達機構の詳細(例えば、センサーと直接反応するのか?あるいは、二次メッセンジャーの生成をもたらすのか?など)は、これまでほとんど分かっていなかった。本研究では、活性酸素・NOの新しい2次メッセンジャーとして発見された8-nitro-cGMPに焦点を当て、その細胞内生成の制御メカニズム、ならびに8-nitro-cGMPによるシグナル伝達機構を明らかにし、活性酸素のシグナルセンシング機構を解明する。8-Nitro-cGMPによるシグナル伝達機能では、特に8-nitro-cGMPによる新しい蛋白質翻訳後修飾である蛋白質S-グアニル化の標的蛋白質の同定と、機能制御メカニズムを明らかにする。

Research Products

• [Journal Article] The critical role of nitric oxide signaling, via protein S-guanylation and nitrated cyclic GMP, in the antioxidant adaptive response (2010)
  • Author(s): Fujii, S., Sawa, T., Ihara, H., Tong, K., Ida, T., Okamoto, T., Ahtesham, A.K., Ishima, Y., Motohashi, H., Yamamoto, M., Akaike, T
  • Journal Title: J.Biol.Chem 285 Pages: 23970-23984
  • Peer Reviewed
• [Journal Article] Cell signaling mediated by nitrated cyclic guanine nucleotide (2010)
  • Author(s): Akaike, T., Fujii, S., Sawa, T., Ihara, H
  • Journal Title: Nitric Oxide 23 Pages: 166-174
  • Peer Reviewed
• [Journal Article] Regulation of redox signaling involving chemical conjugation of protein thiols by nitric oxide and electrophiles (2010)
  • Author(s): Sawa, T., Arimoto, H., Akaike, T
  • Journal Title: Bioconjugate Chem 21 Pages: 1121-1129
  • Peer Reviewed
• [Journal Article] Cytoprotective function of heme oxygenase-1 induced by a nitrated cyclic nucleotide formed during murine salmonellosis (2009)
  • Author(s): Zaki, Md.H., Fujii, S., Okamoto, T., Islam, S., Khan, S., Ahtecham, K., Sawa, T., Akaike, T
  • Journal Title: J.Immunol 182 Pages: 3746-3756
  • Peer Reviewed
• [Journal Article] Methodological proof of immunochemistry for specific identification of 8-nitroguanosine 3', 5'-cyclic monophosphate formed in glia cells
  • Author(s): Ihara, H., Ahmed, K.A., Ida, T., Kasamatsu, S., Kunieda, K., Okamoto, T., Sawa, T., Akaike, T
  • Journal Title: Nitric Oxide in press
  • Peer Reviewed
• [Presentation] Cellular signaling by nitrated cyclic nucleotides and regulation of its biological effects (2011)
  • Author(s): Akaike, T
  • Organizer: Gordon Research Conference on Nitric Oxide
  • Place of Presentation: Ventura,CA,USA
  • Year and Date: 2011-02-17
• [Presentation] Cell signaling mediated by nitrated cyclic guanine nucleotide (2011)
  • Author(s): Akaike, T
  • Organizer: International Symposium on Free Radical Research: Contribution to Medicine
  • Place of Presentation: Kyoto,Japan
  • Year and Date: 2011-01-21
• [Presentation] Protein S-guanylation: a new post-translational regulation for redox signaling (2010)
  • Author(s): Akaike, T
  • Organizer: The 20th World Congress of the International Society for Heart Research 2010 Kyoto
  • Place of Presentation: Kyoto,Japan
  • Year and Date: 2010-05-14
• [Presentation] New NO signaling via 8-nitro-cGMP formation and protein S-guanylation (2009)
  • Author(s): Akaike, T
  • Organizer: 5th Joint Meeting of the Societies for Free Radical Research Australia and Japan
  • Place of Presentation: Sydney,Australia
  • Year and Date: 2009-12-04
• [Presentation] Nitric oxide and electrophilic signaling mediated via 8-nitro-cGMP (2009)
  • Author(s): Akaike, T
  • Organizer: SFRR International Free Radical School in Japan 2009
  • Place of Presentation: Niigata,Japan
  • Year and Date: 2009-09-03
• [Book] Transworld Research Network, T.C Nitric Oxide Synthase Inhibitors: From Animal Studies to Clinical Implications (Tunctan, B., Editor) (2011)
  • Author(s): Okamoto, T., Zaki, M.H., Fujii, S., Sawa, T
  • Publisher: Akaike, T(in press)
• [Book] 実験医学(増刊).病態解明に迫る活性酸素シグナルと酸化ストレス (2009)
  • Author(s): 谷口直之(監修)、赤池孝章、鈴木敬一郎、内田浩二(編集)
  • Total Pages: 241
  • Publisher: 羊土社
• [Book] 診断と治療社、酸化ストレスの医学 (2008)
  • Author(s): 澤 智裕
  • Total Pages: 384 (138-146)
  • Publisher: 赤池孝章
• [Remarks] を開設し、積極的に情報発信を行った
  • URL: http://www.ros-signal.jp/