2013 Fiscal Year Final Research Report
Sequential synthesis of functional synthetic nucleic acids
| Project Area | Organic Synthesis based on Integration of Chemical Reactions. New Methodologies and New Materials |
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| Project/Area Number |
21106007
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
SEKINE MITSUO 東京工業大学, 生命理工学研究科, 教授 (40111679)
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| Project Period (FY) |
2009-07-23 – 2014-03-31
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| Keywords | 連続合成 / マイクロフロー / 固相合成法 / U1snRNA / 2'-O-MCE-RNA / muscular dystrophy / trimethylguanosine cap / RNA transcript |
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| Research Abstract |
In this study, we suceeded in synthesizing the full structure of U1snRNA, which was very difficult to synthesize, by using sequential condensations in the solid-phase. This 5'-terminal 2,2,7-trimethylguanosine cap structure has a function capable of passing through the nuclear membrane from the cytoplasm. The full structure of U1snRNA was constructed for the first time in the world by ligation with an RNA transcript. We also studied the synthesis of 2'-O-MCE-RNA phosphorothioates which have proved to be promising as drugs for treatment of muscular dystrophy. They could be obtained in good yields by using the optimized conditions. A uridine derivative required for this molecule was successfully synthesized by a microflow system.
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[Presentation] Synthesis and Properties of Oligonucleotides Having 2,2,7-Trimethylguanosine Cap Analogs2011
Author(s)
Yamada, K.;Yokouchi, A.;Suzuki, M.; Tsunoda, H.;Ohkubo, A.;Seio, K.;Nagata, T.;Aoki, Y.;Takeda, S.;Sekine, M
Organizer
The 28^<th> International Symposium on Nucleic Acid Chemistry (ISNAC2011)
Place of Presentation
Clark Memorial Student Center, Hokkaido University, Japan
Year and Date
20111109-11
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[Presentation] Oligonucleotides 2,2,7-Trimethylguano- sine Cap Analogues2011
Author(s)
Ohkubo, A.; Yokouchi, A.;Yamada, K.;Tsunoda, H.; Seio, K.;Sekine, M
Organizer
7^<th> Annual Meeting of the Oligonucleotide Therapeutics Society
Place of Presentation
The Royal Library, Copenhagen, Denmark
Year and Date
20110908-09
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[Presentation] Synthesis and Properties of Oligonucleotides 2,2,7-Trimethylguano-sine Cap Analogues2011
Author(s)
Ohkubo, A.; Yokouchi, A.;Yamada, K.;Tsunoda, H.; Seio, K.;Sekine, M
Organizer
6^<th> Cambridge Symposium on Nucleic Acids Chemistry and Biolo
Place of Presentation
Queens'College, Cambridge, UK
Year and Date
20110904-05
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