Molecular mechanisms of allo-recognition in the Brassicaceae

2013 Fiscal Year Final Research Report

• Project Area: Elucidation of Common Mechanisms for Allogeneic Authentication in Animals and Plants
• Project/Area Number: 21112003
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: IWANO Megumi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50160130)
• Project Period (FY): 2009-07-23 – 2014-03-31
• Keywords: アブラナ科植物 / アロ認識 / 自家不和合性 / カルシウムイメージング / シロイヌナズナ / プロトプラスト

Research Abstract

Self-incompatibility (SI) in the Brassicaceae is controlled by the S-haplotype-specific interaction between pollen ligand SP11 and its stigmatic receptor SRK. SP11/SCR binding to cognate SRK induces autophosphorylation of SRK, which triggers a signalling cascade leading to the rejection of self-pollen. Here we introduced calcium sensor protein Yellow Cameleon 3.60 gene into self-incompatible Arabidopsis thaliana transgenic plants expressing both SP11 and SRK. Self-pollination induced an increase of [Ca2+]cyt in papilla cells. The [Ca2+]cyt increase was also induced in papilla cell-protoplasts by directly applying the cognate SP11/SCR. Furthermore, we found that wild type-pollen hydration could also be arrested by the [Ca2+]cyt increase resulting by microinjection of concentrated CaCl2 solution. These results suggest that the SI-induced [Ca2+]cyt increase is a downstream event of SI signal transduction pathway that triggers rejection of self-pollen.

Research Products

• [Journal Article] A pollen coat-inducible autoinhibited Ca^<2+>-ATPase expressed in stigmatic papilla cells is required for compatible pollination in the Brassicaceae (2014)
  • Author(s): Iwano, M., Igarashi, M., Tarutani, Y., Kaothien-Nakayama, P., Nakayama, H., Moriyama, H., Yakabe, R., Entani, T., Shimosato-Asano, H., Ueki, M., Tamiya, G., and Takayama, S
  • Journal Title: Plant Cell Volume: 26 (2) Pages: 636-649
  • DOI: 10.1105/tpc.113.121350
  • Peer Reviewed
• [Journal Article] Ca^<2+>-activated reactive oxygen species production by Arabidopsis RbohH and RbohJ is essential for proper pollen tube tip growth (2014)
  • Author(s): Kaya, H., Nakajima, R., Iwano, M., Kanaoka, M., Kimura, S., Takeda, S., Kawarazaki, T., Senzaki, E., Hamamura, H., Higashiyama, T., Takayama, S., Abe, M., and Kuchitsu, K.
  • Journal Title: Plant Cell Volume: 26 (3) Pages: 1069-1080
  • DOI: 10.1105/tpc.113.120642
  • Peer Reviewed
• [Journal Article] Physiological and Genetic Analysis of CO_2-Induced Breakdown of Self-Incompatibility in Brassica rapa (2014)
  • Author(s): Lao, X., Suwabe, K., Niikura, S., Kakita, M., Iwano, M., and Takayama, S.
  • Journal Title: J Exp Bot. Volume: 65 (4) Pages: 939-951
  • DOI: 10.1093/jxb/ert438
  • Peer Reviewed
• [Journal Article] Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells from a Combination of Laser Microdissection and RNA Sequencing (2013)
  • Author(s): Osaka, M., Matsuda, T., Sakazono, S., Masuko-Suzuki, H., Maeda, S., Sewaki, M., Sone, M., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Shimizu, K.K., Yano, K., Lim, Y.P., Suzuki, G., Suwabe, K., and Watanabe, M.
  • Journal Title: Plant Cell Physiol. Volume: 54 (11) Pages: 1894-1906
  • DOI: 10.1093/pcp/pct133
  • Peer Reviewed
• [Journal Article] Promotion of Cyclic Electron Transport around Photosystem I during the Evolution of NADP-Malic Enzyme-Type C4 Photosynthesis in the Genus Flaveria (2013)
  • Author(s): Nakamura, N., Iwano, M., Havaux, M., Yokota, A., and Munekage, Y.N.
  • Journal Title: New Phytol. Volume: 199 (3) Pages: 832-842
  • DOI: 10.1111/nph.12296
  • Peer Reviewed
• [Journal Article] ATILLING resource for functional genomics in Arabidopsis thaliana accession C24 (2012)
  • Author(s): Lai, K.-S. Kaothien-Nakayama, P., Iwano, M., and Takayama, S.
  • Journal Title: Genes Genet. Syst. Volume: 87 (5) Pages: 291-297
  • DOI: 10.1266/ggs.87.291
  • Peer Reviewed
• [Journal Article] Cytoplasmic Ca^<2+> changes dynamically during the interaction of the pollen tube with synergid cells (2012)
  • Author(s): Iwano, M., Ngo, Q.A., Entani, T., Shiba, H., Nagai, T., Miyawaki, A, Isogai, A, Grossniklaus, U. and Takayama, S.
  • Journal Title: Development Volume: 139 (22) Pages: 4202-4209
  • DOI: 10.1242/dev.081208
  • Peer Reviewed
• [Journal Article] BslA(YuaB) forms a hydrophobic layer on the surface of Bacillus subtilis biofilms (2012)
  • Author(s): Kobayashi, K. and Iwano, M.
  • Journal Title: Mol Microbiol. Volume: 85 (1) Pages: 51-66
  • DOI: 10.1111/j.1365-2958.2012.08094.x
  • Peer Reviewed
• [Journal Article] Self/non-self discrimination in angiosperm self-incompatibility (2012)
  • Author(s): Iwano, M. and Takayama S.
  • Journal Title: Curr Opin Plant Biol. Volume: 15 (1) Pages: 78-83
  • DOI: 10.1016/j.pbi.2011.09.003
  • Peer Reviewed
• [Journal Article] HMG domain containing SSRP1 is required for DNA demethylation and genomic imprinting in Arabidopsis (2011)
  • Author(s): Ikeda, Y., Kinoshita, Y., Susaki, D., Ikeda, Y., Iwano, M., Takayama, S., Higashiyama, T., Kakutani, T. and Kinoshita, T.
  • Journal Title: Dev Cell Volume: 21 (3) Pages: 589-596
  • DOI: 10.1016/j.devcel.2011.08.013
  • Peer Reviewed
• [Journal Article] Glycosylation regulates specific induction of rice immune responses by Acidovorax avenae flagellin (2011)
  • Author(s): Hirai, H., Takai, R., Iwano, M., Nakai, M., Kondo, M., Takayama, S.Isogai, A., and Che, F.S.Hirai, H., Takai, R., Iwano, M., Nakai, M., Kondo, M., Takayama, S.Isogai, A., and Che, F.S.
  • Journal Title: J. Biol. Chem. Volume: 286 (29) Pages: 25519-25530
  • DOI: 10.1074/jbc.M111.254029
  • Peer Reviewed
• [Journal Article] Activity of the C-terminal-dependent vacuolar sorting signal of horseradish peroxidase C1a is enhanced by its secondary structure (2011)
  • Author(s): Matsui, T., Tabayashi, A., Iwano, M., Shinmyo, A., Kato, K., and Nakayama, H.
  • Journal Title: Plant Cell Physiol. Volume: 52 (2) Pages: 413-420
  • DOI: 10.1093/pcp/pcq205
  • Peer Reviewed
• [Journal Article] Trans-acting small RNA determines dominance relationships in Brassica self-incompatibility (2010)
  • Author(s): Tarutani, Y., Shiba, H., Iwano, M., Kakizaki, T., Suzuki, G., Watanabe, M., Isogai, A., and Takayama, S.
  • Journal Title: Nature Volume: 19 (466) Pages: 983-986
  • DOI: 10.1038/nature09308
  • Peer Reviewed
• [Journal Article] アブラナ科植物の他家・自家受粉過程における乳頭細胞液胞の超高圧電顕トモグラフィー解析 (2010)
  • Author(s): 岩野恵、永井里奈、高山誠司
  • Journal Title: 超高圧電子顕微鏡センター年報 Volume: 38 Pages: 44-47
  • Peer Reviewed
• [Journal Article] Fine-tuning of the cytoplasmic Ca^<2+> concentration is essential for pollen tube growth (2009)
  • Author(s): Iwano, M., Entani, T., Shiba, H., Kakita, M., Nagai, T., Mizuno, H., Miyawaki, A., Shoji, T., Kubo, K., Isogai, A., and Takayama, S.
  • Journal Title: Plant Physiol. Volume: 150 (3) Pages: 1322-1334
  • DOI: 10.1104/pp.109.139329
  • Peer Reviewed
• [Journal Article] The transcription factor OsNAC4 is a key positive regulator of plant hypersensitive cell death (2009)
  • Author(s): Kaneda, T., Taga, Y., Takai, R., Iwano, M., Matsui, H., Takayama, S., Isogai, A., and Che, F.S.
  • Journal Title: EMBO J. Volume: 28(7) Pages: 926-936
  • DOI: 10.1038/emboj.2009.39
  • Peer Reviewed
• [Presentation] アブラナ科植物の和合・不和合受粉過程における情報伝達系の解析 (2014)
  • Author(s): 岩野 恵、伊藤 花菜江、浅野(下里)裕子、五十嵐 元子、高山 誠司
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学(富山)
  • Year and Date: 2014-03-18
• [Presentation] アブラナ科植物自家不和合性の分子メカニズム (2013)
  • Author(s): 岩野 恵、伊藤 花菜江、浅野(下里)裕子、五十嵐 元子、岩尾 康宏、高山誠司
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場(神戸)
  • Year and Date: 2013-12-04
• [Presentation] Molecular mechanisms of self-incompatibility in the Brassicaceae (2012)
  • Author(s): Iwano, M
  • Organizer: International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: 名古屋ガーデンパレス(名古屋)
  • Year and Date: 2012-11-14
• [Presentation] アブラナ科植物の自家不和合性の分子機構 (2012)
  • Author(s): 岩野 恵、伊藤 花菜江、浅野(下里)裕子、小池 千恵子、高山 誠司
  • Organizer: 第83回日本動物学会関連集会「受精研究の現在とこれから～普遍性と多様性、そして進化の解明を目指して」
  • Place of Presentation: 大阪大学(大阪)
  • Year and Date: 2012-09-13
• [Presentation] バイオイメージングによるアブラナ科植物自家不和合性の生理学的解析 (2012)
  • Author(s): 岩野 恵、伊藤 花菜江、小池 千恵子、永井 里奈、高山 誠司
  • Organizer: 第68回日本顕微鏡学会シンポジウム「バイオイメージングにより明らかにされた動・植物の有性生殖メカニズム」
  • Place of Presentation: つくば国際会議場(筑波)
  • Year and Date: 2012-05-14
• [Presentation] Molecular mechanisms of self-incompatibility in Brassicaceae (2012)
  • Author(s): Iwano, M.
  • Organizer: XXII International Congress on Sexual Plant Reproduction
  • Place of Presentation: University of Melbourne(Australia)
  • Year and Date: 2012-02-14
• [Presentation] アブラナ科植物の受粉過程におけるCa^<2+>の関与 (2011)
  • Author(s): 岩野 恵
  • Organizer: 第12回医学生物学電子顕微鏡シンポジウム
  • Place of Presentation: 浜松市駅ビル「メイワン」(静岡)
  • Year and Date: 2011-11-26
• [Presentation] アブラナ科植物受粉過程の超高圧電顕トモグラフィー解析 (2011)
  • Author(s): 岩野 恵
  • Organizer: 超高圧電子顕微鏡センター 医学・生物学系共同利用研究報告会
  • Place of Presentation: 大阪大学(大阪)
  • Year and Date: 2011-11-22
• [Presentation] 電子線トモグラフィーによる植物細胞オルガネラの可視化技術の開発 (2011)
  • Author(s): 岩野 恵
  • Organizer: 植物電子顕微鏡若手ワークショップ2011
  • Place of Presentation: 理化学研究所(横浜)
  • Year and Date: 2011-11-21
• [Presentation] バイオイメージングによるアブラナ科植物アロ認識機構の解析 (2009)
  • Author(s): 岩野 恵
  • Organizer: 第32回日本分子生物学会ワークショップ「動植物の受精とアロ認証機構」
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-12-10
• [Presentation] アブラナ科植物におけるアロ認識機構 (2009)
  • Author(s): 岩野 恵
  • Organizer: 日本生化学会第82回大会シンポジウム「動植物におけるアロ認証機構」
  • Place of Presentation: 神戸ポートピアホテル(神戸)
  • Year and Date: 2009-10-23
• [Presentation] Three dimensional analysis of the vacuolar structure in papilla cells during self- and cross-pollination in Brassicaceae (2009)
  • Author(s): Iwano, M.
  • Organizer: 6th International Symposium on Electron Microscopy in Medicine and Biology 2009
  • Place of Presentation: Kobe university, Kobe
  • Year and Date: 2009-09-16
• [Book] 被子植物の受精1-自家不和合性因子の多様性と共通原理(第6章)、動植物の受精学 共通機構と多様性(澤田均 編) (2014)
  • Author(s): 岩野 恵、掛田 克行、土屋 亨
  • Total Pages: 88-102(総ページ数352頁)
  • Publisher: 化学同人
• [Book] Heidelberg, Self-incompatibility in the Brassicaceae, Sexual Reproduction in Animals and Plants (H.Sawada, N.Inoue, M.Iwano : Eds.) (2014)
  • Author(s): Iwano, M., K. Ito, H. Shimosato-Asano, K.S. Lai, S. Takayama
  • Total Pages: 245-254(総ページ数480頁)
  • Publisher: Springer
• [Book] 花粉の世界をのぞいてみたら(医学生物学電子顕微鏡学会編) (2012)
  • Author(s): 岩野 恵
  • Total Pages: 368
  • Publisher: 株式会社エヌ・ティ・エス