Development and application of future technology for allorecognition research

2013 Fiscal Year Final Research Report

• Project Area: Elucidation of Common Mechanisms for Allogeneic Authentication in Animals and Plants
• Project/Area Number: 21112004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Tsukuba
• Principal Investigator: INABA Kazuo 筑波大学, 生命環境系, 教授 (80221779)
• Co-Investigator(Kenkyū-buntansha): SASAKURA Yasunori 筑波大学, 生命環境系, 教授 (10400649)
• Project Period (FY): 2009-07-23 – 2014-03-31
• Keywords: 受精 / 精子走化性 / カラクシン / 海産生物 / トランスジェニック技術 / ホヤ / 鞭毛繊毛

Research Abstract

First, a novel calcium-binding protein calaxin has been shown to bind to the outer arm dynein in a calcium-dependent manner, resulting in suppression of the asymmetric flagellar waveform and in the chemotactic movement of sperm in the ascidian Ciona intestinalis. Second, the eusperm in the gastropod Strombus luhuanus has been shown to exhibit two types of backward swimming. One is to detach from sperm bundle and the other is to be released from the epithelium of female sperm receptacle. In both cases, extracellular calcium ion plays an critical role for switching to backward swimming. Third, by developing analytical systems for high-speed imaging and transgenic techniques, we have conducted many collaborating works in the research group, including allorecognition in the sperm-egg interaction in Ciona, sperm storage mechanism in quail, and regulation of flagellar movement of the zoospore in brown algae.

Research Products

• [Journal Article] カルシウムシグナルによる哺乳類精子超活性化の制御 (2014)
  • Author(s): 稲葉一男
  • Journal Title: 細胞工学 Volume: Vol.33(4) Pages: 366-371
• [Journal Article] Autonomous changes in the swimming direction of sperm in the gastropod Strombus luhuanus (2014)
  • Author(s): Shiba, K., Shibata, D., Inaba, K.
  • Journal Title: J Exp Biol Volume: 217 Pages: 986-996
  • DOI: 10.1242/jeb.095398
  • Peer Reviewed
• [Journal Article] Tissue-specific and ubiquitous gene knockouts in Ciona by electroporating TALENs provide new approaches to investigate gene functions (2014)
  • Author(s): Treen, N., Yoshida, K., Sakuma, T., Sasaki, H., Kawai, N., Yamamoto, T., Sasakura, Y.
  • Journal Title: Development Volume: 141 Pages: 481-487
  • DOI: 10.1242/dev.099572
  • Peer Reviewed
• [Journal Article] Roles of extracellular ions and pH in 5-HTinduced sperm motility in marine bivalve (2013)
  • Author(s): Alavi, S.M., Matsumura, N., Shiba, K., Itoh, N., Takahashi, K.G., Inaba, K., Osada, M.
  • Journal Title: Reproduction Volume: 147 Pages: 331-345
  • DOI: 10.1530/REP-13-0418
  • Peer Reviewed
• [Journal Article] Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in Japanese quail (Coturnix japonica) (2013)
  • Author(s): Hiyama, G., Matsuzaki, M., Mizushima, S., Dohra, H., Ikegami, K., Yoshimura, T., Shiba, K., Inaba, K., Sasanami, T.Hiyama, G., Matsuzaki, M., Mizushima, S., Dohra, H., Ikegami, K., Yoshimura, T., Shiba, K., Inaba, K., Sasanami, T.
  • Journal Title: Reproduction Volume: 47 Pages: 167-178
  • DOI: 10.1530/REP-13-0439
  • Peer Reviewed
• [Journal Article] 精子が卵を目指すしくみ―運動の方向転換を司るタンパク質「カラクシン」 (2013)
  • Author(s): 水野克俊, 柴小菊, 稲葉一男
  • Journal Title: 遺伝 Volume: Vol.67(5) Pages: 610-617
• [Journal Article] Sperm from sneaker male squids exhibit chemotactic swarming to CO2 (2013)
  • Author(s): Hirohashi, N., Alvarez, L., Shiba, K., Fujiwara, E., Iwata, Y., Mohri, T., Inaba, K., Chiba, K., Ochi, H., Supuran, C.T., Kotzur, N., Kakiuchi, Y., Kaupp, U.B., Baba, S.A.
  • Journal Title: Curr Biol Volume: 23 Pages: 775-781
  • DOI: 10.1016/j.cub.2013.03.040
  • Peer Reviewed
• [Journal Article] Efficient TALEN construction and evaluation methods for human cell and animal applications (2013)
  • Author(s): Sakuma, T., Hosoi, S., Woltjen, K., Suzuki, K., Kashiwagi, K., Wada, H., Ochiai, H., Miyamoto, T., Kawai, N., Sasakura, Y., Matsuura, S., Okada, Y., Kawahara, A., Hayashi, S., and Yamamoto, T.
  • Journal Title: Genes to Cells Volume: 18 Pages: 315-326
  • DOI: 10.1111/gtc.12037(
  • Peer Reviewed
• [Journal Article] Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome (2013)
  • Author(s): Hozumi, A., Yoshida, R., Horie, T., Sakuma, T., Yamamoto, T., and Sasakura, Y.
  • Journal Title: Dev Biol Volume: 375 Pages: 79-91
  • DOI: 10.1016/j.ydbio.2012.12.012
  • Peer Reviewed
• [Journal Article] Ascidians as excellent models for studying cellular events in the chordate body plan (2013)
  • Author(s): Ogura, Y., Sasakura, Y.
  • Journal Title: Biol. Bull Volume: 224(3) Pages: 227-236
  • URL: http://www.biolbull.org/content/224/3/227.long
  • Peer Reviewed
• [Journal Article] Calaxin drives sperm chemotaxis by Ca^2□-mediated direct modulation of a dynein motor (2012)
  • Author(s): Mizuno K, Shiba K, Okai M, Takahashi Y, Shitaka Y, Oiwa K, Tanokura M, Inaba K.
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Pages: 20497-20502
  • DOI: 10.1073/pnas.1217018109
  • Peer Reviewed
• [Journal Article] Tubulin-dynein system in flagellar and ciliary movement (2012)
  • Author(s): Mohri, H., Inaba, K., Ishijima, S., Baba, S.A.
  • Journal Title: Proc Jpn Acad Ser B Phys Biol Sci Volume: 88 Pages: 397-415
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/23060230
  • Peer Reviewed
• [Journal Article] Isolation and functional characterization for oocyte maturation and sperm motility of the oocyte maturation arresting factor from the Japanese scallop, Patinopecten yessoensis (2012)
  • Author(s): Yuan, Y., Tanabe, T., Maekawa, F., Inaba, K., Maeda, Y., Itoh, N., Takahashi, K.G., Osada, M.
  • Journal Title: Gen Comp Endocrinol Volume: 179 Pages: 350-357
  • DOI: 10.1016/j.ygcen.2012.09.006
  • Peer Reviewed
• [Journal Article] Self-incompatibility response induced by calcium increase in sperm of the ascidian Ciona intestinalis (2012)
  • Author(s): Saito, T., Shiba, K., Inaba, K., Yamada, L., Sawada, H.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 109(11) Pages: 4158-4162
  • DOI: 10.1073/pnas.1115086109
  • Peer Reviewed
• [Journal Article] Retinoic acid-driven Hox1 is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis (2012)
  • Author(s): Sasakura, Y., Kanda, M., Ikeda, T., Horie, T., Kawai, N., Ogura, Y., Yoshida, R., Hozumi, A., Satoh, N., and Fujiwara, S.
  • Journal Title: Development Volume: 139 Pages: 2156-2160
  • DOI: 10.1242/dev.080234
  • Peer Reviewed
• [Journal Article] Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases (2012)
  • Author(s): Kawai, N., Ochiai, H., Sakuma, T., Yamada, L., Sawada, H., Yamamoto, T., and Sasakura, Y.
  • Journal Title: Dev Growth Differ Volume: 54 Pages: 535-545
  • DOI: 10.1111/j.1440-169X.2012.01355.x
  • Peer Reviewed
• [Journal Article] Sperm flagella : comparative and phylogenetic perspectives of protein components (2011)
  • Author(s): Inaba, K.
  • Journal Title: Mol Hum Reprod Volume: 17 Pages: 524-538
  • DOI: 10.1093/molehr/gar034
  • Peer Reviewed
• [Journal Article] Proteomic profiling reveals compartment-specific, novel functions of ascidian sperm proteins (2011)
  • Author(s): Nakachi, M., Nakajima, A., Nomura, M., Yonezawa, K., Ueno, K., Endo, T., Inaba, K.
  • Journal Title: Mol Reprod Dev Volume: 78 Pages: 529-549
  • DOI: 10.1002/mrd.21341
  • Peer Reviewed
• [Journal Article] Lipid rafts function in Ca2+ signaling responsible for activation of sperm motility and chemotaxis in the ascidian Ciona intestinalisLipid rafts function in Ca2+ signaling responsible for activation of sperm motility and chemotaxis in the ascidian Ciona intestinalis (2011)
  • Author(s): Zhu, L., Inaba, K.
  • Journal Title: Mol Reprod Dev Volume: 78 Pages: 920-929
  • DOI: 10.1002/mrd.21382
  • Peer Reviewed
• [Journal Article] Coordination of mitosis and morphogenesis : role of a prolonged G2 phase during chordate neurulation (2011)
  • Author(s): Ogura, Y., Sakaue-Sawano, A., Nakagawa, M., Satoh, N., Miyawaki, A., and Sasakura, Y.
  • Journal Title: Development Volume: 138 Pages: 577-587
  • DOI: 10.1242/dev.053132
  • Peer Reviewed
• [Journal Article] Ependymal cells of chordate larvae are stem-like cells from the adult nervous system (2011)
  • Author(s): Horie, T., Shinki, R., Ogura, Y., Kusakabe, T.G., Satoh, N., and Sasakura, Y.
  • Journal Title: Nature Volume: 469 Pages: 525-528
  • DOI: 10.1038/nature09631
  • Peer Reviewed
• [Journal Article] CIPRO 2.5 : Ciona intestinalis protein database, a unique integrated repository of large-scale omics data, bioinformatic analyses and curated annotation, with user rating and reniewing functionalit (2011)
  • Author(s): Endo, T., Ueno, K., Yonezawa, K., Mineta, K., Hotta, K., Satou, Y., Yamada, L., Ogasawara, M., Takahashi, H., Nakajima ,A., Nakachi, M., Nomura, M., Yaguchi, J., Sasakura, Y., Yamasaki, C., Sera, M., Yoshizawa, AC., Imanishi, T., Taniguchi ,H., and Inaba ,K.
  • Journal Title: Nicleic Acids Res Volume: 39 (Suppl.1) Pages: 807-814
  • DOI: 10.1093/nar/gkq1144
  • Peer Reviewed
• [Journal Article] Efficient transposition of a single transposon copy in the genome of the ascidian Ciona intestinalis with a transgenic line expressing transposase in eggs (2010)
  • Author(s): Hozumi, A., Kawai, N., Yoshida, R., Ogura, Y., Ohta, N., Satake, H., Satoh, N., and Sasakura, Y.
  • Journal Title: Dev. Dyn Volume: 239 Pages: 1076-1088
  • DOI: 10.1002/dvdy.22254
  • Peer Reviewed
• [Journal Article] Maternal factor-mediated epigenetic gene silencing in the ascidian Ciona intestinalis (2010)
  • Author(s): Sasakura, Y., Suzuki, M. M., Hozumi, A., Inaba, K., and Satoh, N.
  • Journal Title: Mol. Genet. Genomics Volume: 283 (1) Pages: 99-110
  • DOI: 10.1007/s00438-009-0500-4
  • Peer Reviewed
• [Journal Article] Purification of dyneins from sperm flagella (2009)
  • Author(s): Inaba, K. and Mizuno, K.
  • Journal Title: Methods Cell Biol Volume: 92 Pages: 49-63
  • DOI: 10.1016/S0091-679X(08)92004-5
  • Peer Reviewed
• [Presentation] Genome editing with TALENs and Crispr/Cas9 in the ascidian Ciona intestinalis (2014)
  • Author(s): N.Treen, K.Yoshida, H.Sasaki, T.Sakuma, N.Kawai, T.Yamamoto, Y.Sasakura
  • Organizer: International symposium on RNAi and genome editing method
  • Place of Presentation: 徳島大学(徳島)
  • Year and Date: 2014-03-15
  • Invited
• [Presentation] Calaxin is a Ca^<2+>-dependent dynein modulator in opisthokonts : A phylogenetic consideration on the evolution of cilia and flagella (2013)
  • Author(s): Inaba K
  • Organizer: International Workshop Dynein 2013
  • Place of Presentation: Orbis Hall, Kobe, Japan
  • Year and Date: 20131031-1103
  • Invited
• [Presentation] Regulation of ciliary and flagellar movement by a novel calcium sensor calaxin (2013)
  • Author(s): Inaba K
  • Organizer: TLL Seminar
  • Place of Presentation: TEMASEK Lifesciences Laboratory, Singapore
  • Year and Date: 2013-12-09
  • Invited
• [Presentation] 精子運動の方向転換を司るカルシウム結合タンパク質「カラクシン」 : 軸糸ダイニン調節の分子系統学的考察 (2013)
  • Author(s): 稲葉一男
  • Organizer: 第36回日本分子生物学会年会ワークショップ アロ認証から生殖タクティクスへ:動植物域を超えた生殖戦略
  • Place of Presentation: 神戸ポートピアホテル, 神戸
  • Year and Date: 2013-12-03
  • Invited
• [Presentation] 受精時の精子運動性のイメージング (2013)
  • Author(s): 稲葉一男
  • Organizer: 第58回日本生殖医学会学術講演会
  • Place of Presentation: 神戸国際会議場, 神戸
  • Year and Date: 2013-11-15
  • Invited
• [Presentation] 脊索動物ホヤにおける遺伝学的技術を用いた発生メカニズムの解明 (2013)
  • Author(s): 笹倉靖徳
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山大学(岡山)
  • Year and Date: 2013-09-26
  • Invited
• [Presentation] 精子運動の方向を制御するメカニズム (2013)
  • Author(s): 稲葉一男
  • Organizer: 日本アンドロロジー学会第32回学術大会特別講演
  • Place of Presentation: グランキューブ大阪, 大阪
  • Year and Date: 2013-07-26
  • Invited
• [Presentation] Knockout of genes with TALE nucleases in the chordate Ciona intestinalis (2013)
  • Author(s): Y.Sasakura, N.Treen, H.Sasaki, N.Kawai, T.Sakuma, T.Yamamoto, K.Yoshida
  • Organizer: The 46th annual meeting for the Japanese society of developmental biologists
  • Place of Presentation: くにびきメッセ(松江)
  • Year and Date: 2013-05-31
  • Invited
• [Presentation] Mechanism of Ca2+-mediated signaling for sperm motility (2012)
  • Author(s): Inaba K
  • Organizer: The International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: Hotel Nagoya Garden Palace, Nagoya, Japan
  • Year and Date: 20121112-16
  • Invited
• [Presentation] Proteomics, Cell Biology and Physiology for Sperm Flagellar Motility (2011)
  • Author(s): Inaba K
  • Organizer: 6th Asian-Pacific Organization for Cell Biology Congress
  • Place of Presentation: Manila, Phillipines
  • Year and Date: 2011-02-26
  • Invited
• [Book] Structure, function and phylogenetic consideration of calaxin. Sexual Reproduction in Animals and Plants (Ed. by H Sawada) (2014)
  • Author(s): Inaba, K., Mizuno, K., Shiba, K
  • Total Pages: 49-57
  • Publisher: Springer Japan
• [Book] Germline transformation in the ascidian Ciona intestinalis Sexual Reproduction in Animals and Plants (Ed. by H Sawada) (2014)
  • Author(s): Sasakura, Y
  • Total Pages: 465-473
  • Publisher: Springer, Japan
• [Book] Regulatory subunits of axonemal dynein. Handbook of Dynein (eds. Hirose, Amos) (2012)
  • Author(s): Inaba, K
  • Total Pages: 303-324
  • Publisher: PanStanford Publishing
• [Book] Molecular comparison of the axonemal components between sperm flagella and Chlamydomonas flagella. Spermatology : new horizons in the 21 century (ed. M.Moriwsawa) (2012)
  • Author(s): Shiba, K., Mizuno, K., Inaba, K
  • Total Pages: 30-40
  • Publisher: Adthree Publishing Co. Ltd
• [Book] Genome structure, functional genomics, and proteomics in ascidians. In, Genome Mapping and Genomics in Laboratory Animals (eds, P. Denny and C. Kole, Springer) (2012)
  • Author(s): Sasakura Y, Sierro N, Nakai, K Inaba K, Kusakabe TG
  • Total Pages: 87-132
  • Publisher: Springer