2013 Fiscal Year Final Research Report
Regulatory functions of IDPs in the nuclear network of the genetic information systems
| Project Area | Target recognition and expression mechanism of intrinsically disordered protein |
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| Project/Area Number |
21113005
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyushu University |
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Principal Investigator |
ISHINO YOSHIZUMI 九州大学, (連合)農学研究科(研究院), 教授 (30346837)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Mitsuhiro 明星大学, 理工学部, 教授 (80231364)
OKUWAKI Mitsuru 筑波大学, 人間総合科学研究科, 准教授 (50322699)
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| Project Period (FY) |
2009-07-23 – 2014-03-31
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| Keywords | 天然変性蛋白質 / 核内現象 / 遺伝情報制御 / DNA複製 / 転写 / クロマチン / ヒストン |
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| Research Abstract |
Our research has been focusing on the relationships between the chromatin structure and the protein factors involved in DNA replication, repair, and transcription. Many proteins in these pathways contains intrinsically disordered regions (IDR), which should have important functions to process the DNA metabolisms precisely. In this research project, we have characterized Hef, which is involved in the stalled replication fork repair, Ume6, which is a transcription regulation factor, TRF1, which is a telomere binding factor, Nucleophsomin/B23, which is the viral chromatin regulator, and NAP1, which is the histone chaperone. All these proteins contain IDR. We analyzed each IDR for their binding to the various partner molecules and the general conclusion is that IDR have important functions for regulation of the interaction with their partner factors by occasional and sequential folding coupled with binding.
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[Journal Article] Architectures of archaeal GINS complexes, essential DNA replication initiation factors2011
Author(s)
Oyama, T, Ishino, S, Fujino, S, Ogino, H, Shirai, T, Mayanagi, K, Saito, M, Nagasawa, N, Ishino, Y and Y, Morikawa, K
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Journal Title
BMC Biol
Volume: 9
Pages: 28
Peer Reviewed
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[Presentation] Activation mechanism of the replicative helicase in the thermophilic archaeon, Thermoplasma acidophilum2013
Author(s)
Hiromi Ogino, Sonoko Ishino, Kouta Mayanagi, Takuji Oyama, Tsuyoshi Shirai, Kosuke Morikawa, Gyri Teien Haugland, Nils-Kare Birkeland, and Yoshizumi Ishino
Organizer
Thermophiles
Place of Presentation
Regensburg, Germany
Year and Date
20130908-13
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[Presentation] Divergently evolved replicative helicase from the archaeon, Thermoplasma acidophilum2012
Author(s)
Hiromi Ogino, Sonoko Ishino, Kouta Mayanagi, Takuji Oyama, Tsuyoshi Shirai, Kosuke Morikawa, Gyri Teien Haugland, Nils-Kare Birkeland, and Yoshizumi Ishino
Organizer
The 8th 3R symposium
Place of Presentation
Awaji Yumebutai International Conference Center, Japan
Year and Date
20121125-28
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