2013 Fiscal Year Final Research Report
Structural and functional analysis of non-coding RNA machineries
| Project Area | Functional machinery for non-coding RNAs |
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| Project/Area Number |
21115003
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SUZUKI Takeo 東京大学, 工学(系)研究科(研究院), 講師 (90533125)
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| Project Period (FY) |
2009-07-23 – 2014-03-31
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| Keywords | RNA / 転写後修飾 / プロセシング / 修飾酵素 |
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| Research Abstract |
The 5' terminal of Xist RNA, a nuclear retained non-coding RNA which inactivates one of the pair of X chromosomes, was found to be characteristically modified unlike ordinary mRNAs. Also, the conserved element in 3' terminal of Malat1 RNA, the component of a functional nuclear compartment "nuclear speckles", was found to be methylated at an adenosine site. Identification of a gene diminishing the methylated adenosine revealed the most plausible structure and modifying enzymes of the methyladenosine. We developed methylthio-modification measurement method by using small-quantity clinical samples. Taken together, we contributed the findings of RNA structural alterations, including determination of a novel chemical structure, and their modifying factors.
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[Journal Article] Quantitative PCR measurement of tRNA 2-methylthio modification for assessing type 2 diabetes risk2013
Author(s)
Xie, P., Wei, H.-Y., Hirata, S., Kaitsuka, T., Suzuki, T., Suzuki, T. and Tomizawa, K.
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Journal Title
Clin. Chem
Volume: 59
Pages: 1604-1612
DOI
Peer Reviewed
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