2013 Fiscal Year Final Research Report
Regulatory mechanism of Xist RNA machinery
| Project Area | Functional machinery for non-coding RNAs |
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| Project/Area Number |
21115005
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyushu University |
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Principal Investigator |
SADO Takashi 九州大学, 生体防御医学研究所, 准教授 (70321601)
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| Project Period (FY) |
2009-07-23 – 2014-03-31
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| Keywords | 非コードRNA / 発生・分化 / クロマチン / 遺伝学 |
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| Research Abstract |
In this mission, we attempted to figure out the regulatory mechanisms of Xist RNA machinery, which has been known for many years to play a pivotal role in X chromosome inactivation in female mammals. Especially, we focused on the potential role of the proteins, which has been shown to localize to the inactive X, and their interacting partners in Xist RNA-mediated chromatin silencing. The findings indicates the importance of further studies of the potential role of factors involved in replication programming in the Xist RNA machinery. In addition, we have paved the way to our further understanding of the Xist RNA machinery by deriving various cell lines from the mice we established by knocking out a protein involved in the regulation of Xist RNA deposition to the inactive X.
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[Journal Article] Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells2014
Author(s)
Shinagawa T, Takagi T, Tsukamoto D, Tomaru C, Huynh LM, Sivaraman P, Kumarevel T, Inoue K, Nakato R, Katou Y, Sado T, Takahashi S, Ogura A, Shirahige K, Ishii S
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Journal Title
Cell Stem Cell
Volume: 14(2)
Pages: 217-227
DOI
Peer Reviewed
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[Journal Article] Targeted gene silencing in mouse germ cells by insertion of a homologous DNA into a piRNA generating locus2013
Author(s)
Yamamoto Y, Watanabe T, Hoki Y, Shirane K, Li Y, Ichiiyanagi K, Kuramochi-Miyagawa S, Toyoda A, Fujiyama A, Oginuma M, Suzuki H, Sado T, Nakano T, Sasaki H
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Journal Title
Genome Res
Volume: 23(2)
Pages: 292-299
DOI
Peer Reviewed
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[Journal Article] Soloway PD, Sasaki H. Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus2011
Author(s)
Watanabe T, Tomizawa S, Mitsuya K, Totoki Y, Yamamoto Y, Kuramochi-Miyagawa S, Iida N, Hoki Y, Murphy PJ, Toyoda A, Gotoh K, Hiura H, Arima T, Fujiyama A, Sado T, Shibata T, Nakano T, Lin H, Ichiyanagi K
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Journal Title
Science
Volume: 332(6031)
Pages: 848-852
DOI
Peer Reviewed
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[Journal Article] MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline2011
Author(s)
Watanabe T, Chuma S, Yamamoto Y, Kuramochi-Miyagawa S, Totoki Y, Toyoda A, Hoki Y, Fujiyama A, Shibata T, Sado T, Noce T, Nakano T, Nakatsuji N, Lin H, Sasaki H
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Journal Title
Dev Cell
Volume: 20(3)
Pages: 364-375
DOI
Peer Reviewed
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[Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer2010
Author(s)
Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A
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Journal Title
Science.
Volume: 330(6003)
Pages: 496-499
DOI
Peer Reviewed
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