2014 Fiscal Year Final Research Report
Analyses of the activation mechanisms of receptors of fatty acids and membrane receptors by in situ measurement
| Project Area | Integrative understanding of biological processes mediated by transient macromolecular complexes; New technology for visualizing physiologically metastable states. |
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| Project/Area Number |
21121006
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Complex systems
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MORIKAWA Kohsuke 財団法人国際高等研究所, チーフリサーチフェロー (80012665)
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| Co-Investigator(Renkei-kenkyūsha) |
TOCHIO Hidehito 京都大学, 大学院理学研究科, 教授 (70336593)
ARIYOSI Mariko 京都大学, 大学院工学研究科, 博士研究員 (80437243)
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| Project Period (FY) |
2009-04-01 – 2015-03-31
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| Keywords | NMR / 脂質シャペロン / 脂肪酸結合蛋白質 |
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| Free Research Field |
構造生物学、分子生物学
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| Outline of Final Research Achievements |
In this study, we were engaged to develop the methods for in-cell NMR of proteins and optically detected magnetic resonance (ODMR), aiming to create experimental techniques to provide structural and dynamics information of protein complexes formed transiently in cells or at cell membranes. For in-cell NMR, we used the signal transduction factor in immunological system FKBP12 and a chaperon protein for fatty acids, FABP4. We could measure in-cell 1H-15N correlation and 19Ｆ－ＮＭＲ spectra of them, respectively. For ODMR, we have built methods to selectively detect fluorescence of diamond particles and to determine the directions of particles.
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