Development and application of in vivo two-photon microscopy for imaging and stimulation

2014 Fiscal Year Final Research Report

• Project Area: Mutli-dimensional fluorescence live imaging of cellular function and molecular activity
• Project/Area Number: 22113005
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Hokkaido University
• Principal Investigator: NEMOTO Tomomi 北海道大学, 電子科学研究所, 教授 (50291084)
• Co-Investigator(Kenkyū-buntansha): HIBI Terumasa 北海道大学, 電子科学研究所, 助教 (50554292) ; KAWAKAMI Ryosuke 北海道大学, 電子科学研究所, 助教 (40508818) ; OTOMO Kohei 北海道大学, 電子科学研究所, 特任助教 (40547204)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: バイオイメージング / ２光子顕微鏡 / 脳神経科学 / in vivoイメージング / 多光子顕微鏡 / 超短光パルスレーザー

Outline of Final Research Achievements

In order to advance visualization analysis by “in vivo” two-photon microscopy, we combined the microscopy with cutting-edge technologies of laser optics and fluorescent probes novel visualization as well as developed a new analysis technique for estimating optical properties in living bodies. We improved the penetration depth and the spatial resolution in two-photon microscopy, and successfully demonstrated “intact” visualization of hippocampal dentate gyrus in living mouse brains. Furthermore, we created a method for optical manipulation and stimulation, and successfully archived laser ablation of neural fibers within deeper layers in living mouse brains.

Free Research Field

生物物理学