Morphofunctional analysis of regulation of feeding and energy metabolism by feeding regulating peptides

2014 Fiscal Year Final Research Report

• Project Area: Molecular Basis and Disorders of Control of Apetite and Fat Accumulation
• Project/Area Number: 22126004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Showa University
• Principal Investigator: SHIODA SEIJI 昭和大学, 医学部, 教授 (80102375)
• Co-Investigator(Kenkyū-buntansha): KAGEYAMA Haruaki 桐生大学, 医療保健学部, 准教授 (00433839) ; SIBA Kanako 昭和大学, 医学部, 助教 (40551263) ; TAKENOYA Fumiko 星薬科大学, 薬学部, 准教授 (30234412) ; KAN Kison 昭和大学, 医学部, ポストドクター (80613083) ; OGAWA Tetuo 昭和大学, 医学部, 講師 (60384210) ; OOTA Eizi 昭和大学, 医学部・歯学部 (10644263) ; HIRAKO Satoshi 昭和大学, 医学部, ポストドクター (90644261) ; WADA Nobuhiko 昭和大学, 医学部 (30724661)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: 接触調節 / エネルギー代謝 / 機能形態学的解析 / 神経ネットワーク / 遺伝子工学的手法

Outline of Final Research Achievements

We studied a feeding inhibiting neuropeptide Galanin-like peptide (GALP) to identify distribution and localization and afferent neuronal input in brain by use of transgenic (Tg) mice. It appeared that GALP-containing neurons were localized in the hypothalamic arcuate nucleus by Tg mice which expressed GFP under the promoter of GALP gene. Also, GALP-containing neurons were innervated from several neurons distributed in both hypothalamic and extra-hypothalamic regions in mouse brain. We studied the effect of GALP on lipid metabolism in liver. After intraventricular infusion of GALP, the gene expression of several lipid metabolism-relating enzymes and also it appeared the increase of palmitoyl carnitine by a metabolome analysis. These findings were completely canceled by treatment of guanitidine which blocked the sympathetic nerve. These may indicate that GALP inhibits appetite through sympathetic nervous system and it also affects on lipid metabolism in liver and adipose tissue..

Free Research Field

医歯薬学