Three dimensional tissue regeneration through multipoint molecular weak association

2015 Fiscal Year Final Research Report

• Project Area: Molecular Science for Nanomedicine
• Project/Area Number: 23107008
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Kyoto University
• Principal Investigator: IWATA Hiroo 京都大学, 再生医科学研究所, 名誉教授 (30160120)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Yukihiro 大阪大学, 基礎工学研究科, 講師 (50503918) ; KITAMURA Narufumi 京都大学, 再生医科学研究所, 研究員 (50624912) ; ARIMA Yusuke 京都大学, 再生医科学研究所, 助教 (90402792)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 細胞 / 単鎖DNA / リン脂質 / 細胞表面修飾 / 分子間相互作用 / 細胞配置 / 生体応答

Outline of Final Research Achievements

The aim of this study is manipulation of cellular function through the modification of cell surface. We used single-stranded DNA, which was conjugated both to poly(ethylene glycol) and to a terminal phospholipid (ssDNA-PEG-lipid) for cell surface modification. The cell surface of cells can be further functionalized through DNA hybridization. We examined control of 1) biological responses and 2) cell-substrate and cell-cell attachment.
1) The modification of cell surface of endothelial cells with antioxidant-loaded liposomes reduced oxidative stress during ischemia reperfusion. The modification of cell aggregates with superparamagnetic iron oxide nanoparticles allowed for MRI monitoring post-transplantation.
2) We realized cell attachment to poly(lactic acid) scaffold in a spatially controlled manner by taking advantage of variety of DNA sequence. In addition, both cell attachment and detachment can be programmed using DNA containing the cleavage site for restriction enzyme.

Free Research Field

高分子医工学