Molecular mechanisms of incest avoidance and reproductive isolation occurring in different genomes within species

2015 Fiscal Year Final Research Report

• Project Area: Correlative gene system: establishing next-generation genetics
• Project/Area Number: 23113006
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka Kyoiku University
• Principal Investigator: Suzuki Go 大阪教育大学, 教育学部, 教授 (10314444)
• Co-Investigator(Kenkyū-buntansha): WATANABE Masao 東北大学, 大学院生命科学研究科, 教授 (90240522) ; SUWABE Keita 三重大学, 大学院生物資源学研究科, 准教授 (50451612)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 遺伝学 / 高等植物 / インセスト回避 / 生殖隔離 / 低分子RNA / 雄性生殖器官 / 遺伝子機能 / 分子間相互作用

Outline of Final Research Achievements

We proceeded according to three projects to elucidate correlative gene systems during plant pollination. At first, molecular evolution of self-compatibility in Arabidopsis was discussed from results of restoring incest avoidance using transgenic experiments. Secondly, we identified a set of female and male determinant genes for novel reproductive isolation in Brassica rapa, and demonstrated their functions in this incompatibility system. Thirdly, platforms of comprehensive transcriptome data during pollination were constructed in rice and Brassica plants. By using miRNA profiles in rice anther, we found the miRNA-mediated gene regulation in the cool tolerant rice cultivar. In addition to these experiments, we also performed outreach activities to elementary-school, and high-school students.

Free Research Field

分子生物学