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2015 Fiscal Year Final Research Report

Technical basis for artificial modification and transplantation of organelles

Planned Research

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Project Area"Matryoshka"-type evolution of eukaryotes
Project/Area Number 23117009
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKobe University

Principal Investigator

Suzaki Toshinobu  神戸大学, 理学(系)研究科(研究院), 准教授 (00187692)

Co-Investigator(Kenkyū-buntansha) TACHIBANA Hiroshi  東海大学, 医学部, 教授 (10147168)
Co-Investigator(Renkei-kenkyūsha) YOSHIMURA Chisato  神戸大学, 環境保全推進センター, 助教 (60362761)
Project Period (FY) 2011-04-01 – 2016-03-31
Keywords細胞内共生 / 原生生物 / ミトコンドリア / マイクロインジェクション / オルガネラ
Outline of Final Research Achievements

A green ciliate Paramecium bursaria harbors several hundred Chlorella cells in its cytoplasm. An electron microscopic approach with 3-D reconstruction showed that mitochondria in the host's cytoplasm provide a structural scaffold for symbiotic Chlorella to be anchored in the host's cytoplasm. Proteome and de novo transcriptome sequencing analyses were carried out to identify proteins in the peri-algal membrane fraction. As a distinctive aspect of the unicellular Matryoshka-type protists, P. bursaria was also found to accumulate soil-bound radioactive cesium.
Anaerobic protozoan Entamoeba species have mitosomes that are mitochondrion-derived organelles lacking DNA. Mitosomes isolated from E. histolytica were transplanted successfully into E. histolytica, E. nuttalli and E. invadens. Co-localization of mitosomal proteins from donor and recipient was observed in single mitosomes of some transplanted cells.

Free Research Field

細胞生物学、原生生物学

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Published: 2017-05-10  

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