1989 Fiscal Year Annual Research Report

ホヤにおける「種」の総合的研究

Research Project

Project/Area Number	01304007
Research Institution	Kochi University
Principal Investigator	中内光昭高知大学, 学長 (00036526)
Co-Investigator(Kenkyū-buntansha)	道端齊富山大学, 理学部, 助教授 (00111740) 佐藤矩行京都大学, 理学部, 助教授 (30025481) 星元紀東京工業大学, 理学部, 教授 (20012411) 星野善一郎岩手大学, 教育学部, 教授 (30004592) 沼宮内隆晴東北大学, 理学部, 助教授 (90004324)
Keywords	種 / ホヤ / 原索動物 / 非癒合反応 / バナジウム
Research Abstract	本年度の研究は、ユウレイボヤ類、マボヤ、イタボヤ類の3群を中心に行われたが、重金属濃縮機構に関してはさらに対象を拡げた。ユウレイボヤ類では、ユウレイボヤとカタユウレイボヤについて、まず眼点の構造を比較し、両種で繊毛基底小体様構造物を見いだすと共に細胞表面の突出構造が両種で違うことを見いだした。ユウレイボヤでのアイソザイムパタ-ン解析は、泳動及び染色条件の検討を終わった。さらに、DNA(特に、rDNA)の塩基配列を指標に種の比較を行った。受精に関する両種の比較では、受精時の精子活性化物質や細胞認識に関与する分子に微妙な差があることを示唆するデ-タが得られた。マボヤでは、配偶子放出のみならず、卵成熟と光との関係が3型間で異なっているらしいことが判明した。18sリボゾ-ムRNAの比較は、技術的問題があり、その解決を急いでいる。イタボヤ類では、大西洋岸(Woods Hole)と太平洋岸(Monterey)のウスイタボヤの多面的な比較を行い、両者は形態・生殖・発生様式・生活史では差が認められないが、非癒合反応の様式が異なり、両者間での癒合も認められないことがわかった。さらに、Botryllus属の生殖、発生様式、生活史及び、非癒合反応を比較する過程で、新種(B.puniceus)を発見した。重金属濃縮での種間差異に関する研究では、バナ-ドビン(V結合物質)を5種のホヤから抽出し比較した。本物質の分子量、吸光度、V含有量は種で若干異なるが、Vを含むホヤでは普遍的な物質であるらしいことがわかった。さらに、Ascidia gemmataの血球細胞は海水の400万倍のVを含むことも発見した。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] Kajiwara,S.: "Retinal isomers in the cerebral ganglion of the ascidian,Halocynthia roretzi." International Journal of Invertebrate Reproduction and Development.
[Publications] Pinto,M.R.: "Chymotrypsin-like enzymes are involved in sperm penetration through the vitelline coat of Ciona intestinalis egg." Gamete Res.(1990)
[Publications] Michibata,H.: "Isolation of highly acidic and vanalium-containing blood cells from several types of blood cells in Ascidiidae species by density-gradient centrifugation" J.Exp.Zool.
[Publications] Michibata,H.: "Valency of vanadium in the vanadocytes of Ascidia gemmata separated by density-gradient centrifugation." J.Exp.Zool.
[Publications] Michibata,H.: "Extraction of a vanadium-binding substrate(vanadobin)from the blood cells of several ascidian species." Biological Bulletin.
[Publications] Michibata,H.: "Vanadium-containing blood cells(vanadocytes)show no fluorescence due to the tunichrome in the ascidian,Ascidia sydneiensis samea." Zoological Science. (1990)
[Publications] Michibata,H.: "Extraction of vanadium-binding substance(vanadobin)from a subpopulation of signet ring cells newly identified as vanadocytes in ascidians." J.Exp.Zool.(1990)
[Publications] Michibata,H.: "New aspects of accumulation and reduction of vanadium ions in ascidian,based on concerted investigation from both a chemical and biological viewpoint." Zoological Science. 6. 639-647 (1989)
[Publications] Nishide,K.: "A monoclonal antibody specific to embryonic trunk-lateral cells of the ascidian Halocynthia roretzi stains coelomic cells of juvenile and adult basophilic blood cells." Develop.Growth & Differ.31. 595-600 (1989)
[Publications] Nishikata,T.: "Differentiation of trunk-lateral mesenchyme cells in ascidian embryos analysed with a specific monoclonal antibody." J.Exp.Zool.(1990)
[Publications] Satoh,N.: "Cellular and molecular mechanisms of muscle cell differentiation in ascidian embryos." Intern.Review Cytol.122. (1990)
[Publications] Hirose,E.: "Minute protrusions of cuticle:fine surface structures of the tunic in ascidians." J.Morph.(1990)
[Publications] Hirose,E.: "Allogeneic rejection induced by cut surface contact in the compound ascdian,Botrylloides simodensis." Invertebr.Reprod.Dev.(1990)
[Publications] Boyd,H.C.: "Morphologic and genetic verification that Monterey Botryllus and Woods Hole Botryllus are the same species." Biological Bulletin.
[Publications] Kawamura,K.: "Concanavalin A modifies allo-specific sperm-egg interactions in the ascidian,Ciona intestinalis." Develop.Growth & Differ.31. 493-501 (1989)
[Publications] Kawamura,K.: "Self incompatibility-determing activity extracted from Ciona intestinalis eggs." J.Cell Biol.
[Publications] Michibata,H.: "Vanadium in Biological Systems(Edit.by N.D.Chasteen)“Vanadium in ascidians"分担執筆" Kluwer Acad.Publ,Dordrecht,Netherland, (1990)
[Publications] 道端齊: "松永是編「マリンバイオ-海洋バイオ新素材・新物質」“バナドビン"分担執筆" シ-・エム・シ-(東京), 6ペ-ジ (1989)

1989 Fiscal Year Annual Research Report

ホヤにおける「種」の総合的研究

Principal Investigator

中内 光昭 高知大学, 学長 (00036526)

Research Products

[Publications] Kajiwara,S.: "Retinal isomers in the cerebral ganglion of the ascidian,Halocynthia roretzi." International Journal of Invertebrate Reproduction and Development.

[Publications] Pinto,M.R.: "Chymotrypsin-like enzymes are involved in sperm penetration through the vitelline coat of Ciona intestinalis egg." Gamete Res.(1990)

[Publications] Michibata,H.: "Isolation of highly acidic and vanalium-containing blood cells from several types of blood cells in Ascidiidae species by density-gradient centrifugation" J.Exp.Zool.

[Publications] Michibata,H.: "Valency of vanadium in the vanadocytes of Ascidia gemmata separated by density-gradient centrifugation." J.Exp.Zool.

[Publications] Michibata,H.: "Extraction of a vanadium-binding substrate(vanadobin)from the blood cells of several ascidian species." Biological Bulletin.

[Publications] Michibata,H.: "Vanadium-containing blood cells(vanadocytes)show no fluorescence due to the tunichrome in the ascidian,Ascidia sydneiensis samea." Zoological Science. (1990)

[Publications] Michibata,H.: "Extraction of vanadium-binding substance(vanadobin)from a subpopulation of signet ring cells newly identified as vanadocytes in ascidians." J.Exp.Zool.(1990)

[Publications] Michibata,H.: "New aspects of accumulation and reduction of vanadium ions in ascidian,based on concerted investigation from both a chemical and biological viewpoint." Zoological Science. 6. 639-647 (1989)

[Publications] Nishide,K.: "A monoclonal antibody specific to embryonic trunk-lateral cells of the ascidian Halocynthia roretzi stains coelomic cells of juvenile and adult basophilic blood cells." Develop.Growth & Differ.31. 595-600 (1989)

[Publications] Nishikata,T.: "Differentiation of trunk-lateral mesenchyme cells in ascidian embryos analysed with a specific monoclonal antibody." J.Exp.Zool.(1990)

[Publications] Satoh,N.: "Cellular and molecular mechanisms of muscle cell differentiation in ascidian embryos." Intern.Review Cytol.122. (1990)

[Publications] Hirose,E.: "Minute protrusions of cuticle:fine surface structures of the tunic in ascidians." J.Morph.(1990)

[Publications] Hirose,E.: "Allogeneic rejection induced by cut surface contact in the compound ascdian,Botrylloides simodensis." Invertebr.Reprod.Dev.(1990)

[Publications] Boyd,H.C.: "Morphologic and genetic verification that Monterey Botryllus and Woods Hole Botryllus are the same species." Biological Bulletin.

[Publications] Kawamura,K.: "Concanavalin A modifies allo-specific sperm-egg interactions in the ascidian,Ciona intestinalis." Develop.Growth & Differ.31. 493-501 (1989)

[Publications] Kawamura,K.: "Self incompatibility-determing activity extracted from Ciona intestinalis eggs." J.Cell Biol.

[Publications] Michibata,H.: "Vanadium in Biological Systems(Edit.by N.D.Chasteen)“Vanadium in ascidians"分担執筆" Kluwer Acad.Publ,Dordrecht,Netherland, (1990)

[Publications] 道端齊: "松永是編「マリンバイオ-海洋バイオ新素材・新物質」“バナドビン"分担執筆" シ-・エム・シ-(東京), 6ペ-ジ (1989)

中内光昭高知大学, 学長 (00036526)