1991 Fiscal Year Annual Research Report

ホヤにおける「種」の総合的研究

Research Project

Project/Area Number	01304007
Research Institution	Kochi University
Principal Investigator	中内光昭高知大学, 学長 (00036526)
Co-Investigator(Kenkyū-buntansha)	道端齊広島大学, 理学部, 助教授 (00111740) 佐藤矩行京都大学, 理学部, 助教授 (30025481) 星元紀東京工業大学, 生命理工学部, 教授 (20012411) 星野善一郎岩手大学, 教育学部, 教授 (30004592) 沼宮内隆晴東北大学, 理学部, 助教授 (90004324)
Keywords	ホヤ / 種 / rDNA / マボヤ / ユウレイボヤ / イタボヤ
Research Abstract	過去2年間の経験と成果をふまえ,大別して,次の4つの方面から,ホヤの種に対してアプロ-チを行った。 1.遺伝子レベルでのホヤの種の検討昨年度クロ-ニングしたユウレイボヤのrDNAを用い,被検個体のDNA断片との分子交雑により,各地のユウレイボヤ,カタユウレイボヤの遺伝的多型を調べた。その結果,両種ともに遺伝的多型が認められたが,カタユウレイボヤに比べて,ユウレイボヤの方が変異が大きく,進化が速いものと予想された。次に,18S rDNAの中央部約1.1kbの塩基配列で7種のホヤを比較した。その結果,腸性目と壁性目の間の差が明瞭なことや,両者の分岐年代を推定するくとができた。さらに,マボヤ3型のmtDNAで,型特有の泳動パタ-ンを見つけた。 2.生理機能からみたホヤの種マボヤ3型のF1比較は飼育困難のため,結果を出すことができなかったが,3型間で,卵成熟,自家不和合性の確立機構に差が認められた。さらに,この研究の副産物として,卵成熟抑制物質の抽出に成功した。バナジウム濃縮機構については,印環細胞特異的な抗体を用い,濃縮と細胞分化との関係を明らかにした。 3.微細構造からみたホヤの種精子,眼点,被嚢の微細構造を多くの種で比較し,共通的な構造と種特異的な構造とにわけることができた。 4.新しい視点からのホヤの系統分類の再検討イタボヤ類について総合的に検討し,1新属,4新種を明らかにすると共に,イタボヤ亜科全体の進化傾向についても新説を提示できるようになった。さらに,欧州のピウラ属についても整理を行なうことができた。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] Michibata,H.et.al.: "Uptake of ^<48>Vーlabeled vanadium by subpopulations of bloodーcells in the ascidian,Ascidia gemmata." Zoological Science. 8. 447-452 (1991)
[Publications] Michibata,H.et al.: "Vanadobin,a vanadiumーbiding substance,extracted from the blood cells of an ascidian,can reduce vanadate(V) to vanadyl(IV)." Biological Bulletin. 181. 189-194 (1991)
[Publications] Hirose,E.et al.: "An ultrastructural study on the origin of glomerulocytes,a type of blood cell in a styelid ascidian Polyandrocarpa misakiensis." Journal of Morphology. (1992)
[Publications] Fukumoto,M.: "The acrosome reaction in Ciona intestinalis(Ascidia,Tunicata)" Development,Growth,Differentiation. 32. 51-55 (1991)
[Publications] Fukumoto,M.: "Morphological aspects of ascidian fertilization." Zoological Science. 7. 989-998 (1990)
[Publications] Fuke,M.et al.: "Fine structure of blood cells of the solitary ascidian,Halocynthia roretzi,with reference to their behavior in vitro." Acta Zoologica. (1992)
[Publications] Ueki,T.et al.: "Isolation of cDNA clones for epiedermisーspecific genes of the ascidian embryo." Dev.Growth Differ.33. 579-586 (1991)
[Publications] Nishikata,T.et al.: "Expression of an antigen specific for trunk lateral cells in quarter embryos of the ascidian,Halocynthia roretzi." Journal of Experimental Zoology. 258. 344-352 (1991)
[Publications] Robinson,W.E.et al.: "Glutathione effects Ciona intestinalis larval tunic morphogenesis." Journal of Experimental Zoology. 257. 58-63 (1991)
[Publications] Hirata,J.et al.: "Valency of vanadium in the vanadocytes of Ascidia gemmata separted by densityーgradient centrifugation." Journal of Experimental Zoology. 257. 160-165 (1991)
[Publications] Michibata,H.et al.: "Isolation of highly acidic and vanadiumーcontaining blood cells from among several types of blood cells in Ascidiidae species by densityーgradient centrifugation." Journal of Experimental Zoology. 257. 306-313 (1991)
[Publications] Uyama,T.et al.: "Monoclonal antibody specific to signet ring cells,the vanadocyes of the tunicate,Ascidia sydneiensis samea." Journal of Experimental Zoology. 259. 196-201 (1991)
[Publications] Kawamura,K.et al.: "Homeostatic integration of stem cell dynamics during palleal budding of ascidians." Zoological Science. 8. 11-22 (1991)
[Publications] Boyd,H.et al.: "Morphologic and genetic verification that Monterey Botryllus and Wood Hole Botryllus are the same species." Biological Bulletin. 178. 239-250 (1990)
[Publications] Hirose,E.et al.: "Tunic cell morphology and classification in botryllid ascidians." Zoological Science. 8. 951-958 (1991)
[Publications] Hirose,E.et al.: "Minute protrusions of ascidian tunic cuticle:some implications for ascidian phylogeny." Zoological Science. 9. (1992)
[Publications] Saiga,H.et al.: "Molecular cloning and expression of a novel homeobox gene AHoxl of the ascidian,Halocynthia roretzi." Development. 111. 821-828 (1991)
[Publications] Marikawa,Y.et al.: "Gray and red fragments of the egg of the ascidian Ciona savignyi:Preferential development of muscle cells from gray fragments." Dev.Growth Differ.33. 307-316 (1991)

1991 Fiscal Year Annual Research Report

ホヤにおける「種」の総合的研究

Principal Investigator

中内 光昭 高知大学, 学長 (00036526)

Research Products

[Publications] Michibata,H.et.al.: "Uptake of ^<48>Vーlabeled vanadium by subpopulations of bloodーcells in the ascidian,Ascidia gemmata." Zoological Science. 8. 447-452 (1991)

[Publications] Michibata,H.et al.: "Vanadobin,a vanadiumーbiding substance,extracted from the blood cells of an ascidian,can reduce vanadate(V) to vanadyl(IV)." Biological Bulletin. 181. 189-194 (1991)

[Publications] Hirose,E.et al.: "An ultrastructural study on the origin of glomerulocytes,a type of blood cell in a styelid ascidian Polyandrocarpa misakiensis." Journal of Morphology. (1992)

[Publications] Fukumoto,M.: "The acrosome reaction in Ciona intestinalis(Ascidia,Tunicata)" Development,Growth,Differentiation. 32. 51-55 (1991)

[Publications] Fukumoto,M.: "Morphological aspects of ascidian fertilization." Zoological Science. 7. 989-998 (1990)

[Publications] Fuke,M.et al.: "Fine structure of blood cells of the solitary ascidian,Halocynthia roretzi,with reference to their behavior in vitro." Acta Zoologica. (1992)

[Publications] Ueki,T.et al.: "Isolation of cDNA clones for epiedermisーspecific genes of the ascidian embryo." Dev.Growth Differ.33. 579-586 (1991)

[Publications] Nishikata,T.et al.: "Expression of an antigen specific for trunk lateral cells in quarter embryos of the ascidian,Halocynthia roretzi." Journal of Experimental Zoology. 258. 344-352 (1991)

[Publications] Robinson,W.E.et al.: "Glutathione effects Ciona intestinalis larval tunic morphogenesis." Journal of Experimental Zoology. 257. 58-63 (1991)

[Publications] Hirata,J.et al.: "Valency of vanadium in the vanadocytes of Ascidia gemmata separted by densityーgradient centrifugation." Journal of Experimental Zoology. 257. 160-165 (1991)

[Publications] Michibata,H.et al.: "Isolation of highly acidic and vanadiumーcontaining blood cells from among several types of blood cells in Ascidiidae species by densityーgradient centrifugation." Journal of Experimental Zoology. 257. 306-313 (1991)

[Publications] Uyama,T.et al.: "Monoclonal antibody specific to signet ring cells,the vanadocyes of the tunicate,Ascidia sydneiensis samea." Journal of Experimental Zoology. 259. 196-201 (1991)

[Publications] Kawamura,K.et al.: "Homeostatic integration of stem cell dynamics during palleal budding of ascidians." Zoological Science. 8. 11-22 (1991)

[Publications] Boyd,H.et al.: "Morphologic and genetic verification that Monterey Botryllus and Wood Hole Botryllus are the same species." Biological Bulletin. 178. 239-250 (1990)

[Publications] Hirose,E.et al.: "Tunic cell morphology and classification in botryllid ascidians." Zoological Science. 8. 951-958 (1991)

[Publications] Hirose,E.et al.: "Minute protrusions of ascidian tunic cuticle:some implications for ascidian phylogeny." Zoological Science. 9. (1992)

[Publications] Saiga,H.et al.: "Molecular cloning and expression of a novel homeobox gene AHoxl of the ascidian,Halocynthia roretzi." Development. 111. 821-828 (1991)

[Publications] Marikawa,Y.et al.: "Gray and red fragments of the egg of the ascidian Ciona savignyi:Preferential development of muscle cells from gray fragments." Dev.Growth Differ.33. 307-316 (1991)

中内光昭高知大学, 学長 (00036526)