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1990 Fiscal Year Final Research Report Summary

Construction of the Rat Chromosomal Map by DNA Probes.

Research Project

Project/Area Number 01440088
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionKyoto University

Principal Investigator

YAMADA Junzo  Faculty of Medicine, Kyoto University, Professor, 医学部, 教授 (90025651)

Co-Investigator(Kenkyū-buntansha) SERIKAWA Tadao  Faculty of Medicine, Kyoto University, Associate Professor, 医学部, 助教授 (30025655)
Project Period (FY) 1989 – 1990
Keywordsrat / DNA probe / situ hybridization / gene mapping / RFLP / backcross analysis / somatic cell hybridization
Research Abstract

1. Chromosomal assignment of the rat genes by DNA probes, backcross analysis, and in situ hybridization. (1) Angiotensinogen (Ang) : 3 alleles of Ang were detected among inbred strains of rats by using the cDNA and Southern blot analysis. Ang was not linked with other 16 loci using 2 sets of backcross analysis, but was assigned to 19q region by in situ hybridization. (2) Insulin-1 (Ins-1) : Ins-1 was assigned to 1q55 by fluorescence in situ hybridization (FISH) , and 4 alleles of the locus were detected by Southern blot analysis. Ins-1 was not inked with other 22 loci including c, Hbb which located on chromosome 1. This discrepancy may be due to the remote distance between them on the chromosome. (3) Renin (Ren) : Ren was assigned to 13ql by FISH. Since Ren gene have a VNTR within the 1st intoron of the gene, 5 alleles were detected among 11 inbred strains of rats. Backcross analysis revealed the linkages of Ren with Fh (20cM) and Pep-3 (13cM). The order on chromosome 13 was determined as Fh - Ren - Pep-3. (4) beta2-microglobulin (B2m) : 3 RFLPs by using B2m probe were detected by Southern blot analysis, and backcross analysis revealed the linkage of B2m locus with zi (2 or 9cM), Hao-1 (6cM) , and Srp-1 (29cm) . B2m was assigned to chromosome 3 by a panel of 18 somatic cell hybrid clones made by mouse and rat cells.
2. Chromosomal assignment of biochemical genes by somatic cell hybrid clones. We made a panel of 18 somatic cell hybrid clones between mouse and rat cells. This panel enabled us to newly assign 21 biochemical genes of rats on chromosome 1, 2, 3, 4, 7, 8, 9, 10, 12, 13, 14, 19, and 20, respectively. Further Acp-1 and Glo-1 were reconfirmed to locate on chromosome 6 and 20, respectively.

  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Kondo,Y.: "Genetic polymorphism of tear proteins in the rat." Biochemical Genetics. 26. 683-691 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori,M.: "RFLPs of angiotensinogen gene in inbred strains of ratsandmapping of the gene on chromosome 19q." Cytogenetics and Cell Genetics. 50. 42-45 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada,T.: "Assignment of body tremulous mutant gene zitter (zi) to linkege group IV of the rat (Rattus norvegicous)." Journal of Heredity. 80. 383-386 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kondo,Y.: "Second genetic polymorphism of tear proteins in the rat (Rtpー2)." Experimental Animals. 38. 327-331 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Higashiguchi,T.: "Identification of inbred strains of rats by DNA fingerprints using enhanced chemiluminescence." Transplantaion Proceedings. 22. 2564-2565 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori,M.: "Precise mapping on chromosome 1q55 of rat insulin I gene by fluorescence in in situhybridization." Cytognetics and Cell Genetics.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasue,M.: "Chromosomal assignment of 23 biochemical loci of the rat by using rat x mous somatic cell hybrids." Cytogenetics and Cell Genetics.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori,M.: "Variable number of tandem repeat (VNTR) of the rat renin gene, linkage of the gene on linkage group X and assignment of the gene on chromosome 13q1." Genomics.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Higashiguchi,T.: "Semiーdominant expression of absenceーlike seizure in tremor rat." Behavior Genetics.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田 淳三 (編著): "実験動物の生物学的特性デ-タ 7.遺伝子・染色体に関するデ-タ" ソフトサイエンス社, 76 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田 淳三 (分担執筆): "実験動物事典" 朝倉書店, 507 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田 淳三: "毒性試験に用いられる実験動物 1.2 ラット" 地人書館, 12 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 芹川 忠夫: "実験動物の基礎と技術 II 各論 10.2 ラット" 丸善, 12 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kondo, Y.: "Genetic polymorphism of tear protein in the rat." Biochem. Genet.26. 683-691 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori, M.: "RFLPs of angiotensinogen gene in inbred straoms of rats and mapping of the gene on chromosome 19q." Cytogenet. Cell Genet.50. 42-45 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, T.: "Assignment of body tremulous mutant gene zitter (zi) to linkage group IV of the rat (Rattus norvegicus)" J. Hered.80. 383-386 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kondo, Y.: "Second genetic polymorphism of tear proteins in the rat (rtp-2)" Exp. Anim.38. 327-331 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Higashiguchi, T.: "Identification of inbred strains of rats by DNA fingerprints using enhanced chemiluminescence." Transpl. Proc.22. 2564-2565 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori, M.: "Precise mapping on chromosome 1q55 of rat insulin I gene by fluorescence in situ hybridization." Cytogenet. Cell Genet.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasue, M.: "Chromosomal assignment of 23 biochemical loci of the rat by using rat x mouse somatic cell hybrids." Cytogenet. Cell Genet.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori, M.: "Variable number of tandem repeat (VNTR) of the rat renin gene, linkage of the gene on linkage group X and assignment of the gene on chromosome 13q1." Genomics.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Higashiguchi, T.: "Semi-dominant expression of absence-like seizure in tremor rat." Behavior Genet.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, J.: Gustav Fischer Verlag. Genetic Monitoring of Inbred Strains of Rats. ed. by H. J. Hedrich., (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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