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1990 Fiscal Year Final Research Report Summary

Signal Transduction of Bioactive Carbohydrate Chains and Their Genetic Expression

Research Project

Project/Area Number 01440091
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionUniversity of Tokyo

Principal Investigator

NAGAI Yoshitaka  東京大学, 医学部・医学科, 教授 (80072974)

Co-Investigator(Kenkyū-buntansha) OSANAI Taka  University of Tokyo, Fac. Med., Research Associate, 医学部・医学科, 教務職員 (60126018)
TSUJI Shuichi  University of Tokyo, Fac. Med., Research Associate, 医学部・医学科, 助手 (90124677)
SANAI Yutaka  University of Tokyo, Fac. Med., Research Associate, 医学部・医学科, 助手 (40150289)
IWAMORI Masao  University of Tokyo, Fac. Med., Associate Professor, 医学部・医学科, 助教授 (90110022)
NAGAI Yoshitaka  University of Tokyo, Fac. Med., Professor (80072974)
Project Period (FY) 1989 – 1990
KeywordsGanglioside / Oncogene / Ecto-phosphorylation / Uterine endometrium / Keratinization / Sulfatide / Sulfotransferase / Lipokerachinogenoside
Research Abstract

During the period of investigation on the mechanism of signal transduction through carbohydrate chain and of the gene-expression, the following findings were obtained ;
1 Membrane-associated glycoproteins which were characteristically phoshporylated in response with GQlb ganglioside were found in human neuroblastoma cells, whose neurite was extended with GQlb ganglioside. These kinases were inhibited with an impermeable inhibitor for protein kinase, which was also inhibited the activity of GQlb-mediated neurite outgrowth of the cells. The results indicate an occurrence of carbohydrate chain dependent signal transduction system.
2 By gene-transfection technique, the characteristic expression of glycosphingolipids, such as GD3 by adenovirus ElA and myc, and lacto-and ganglio-series glycolipids by src, fes, fps and ras oncogenes, were observed in rat 3Yl cells, indicating that the different oncogene products modify the glycolipid metabolism in their own manners.
3 When compared the proliferative and the secretory phases of human uterine endometrium, a characteristic induction of sulfatide was observed in the secretory phase. Since the ganglioside contents in both phases were identical each other, a ngative charge due to the sulfate group was added in the secretory phase, in which the fertilized egg was trapped and embedded. Probably, the sulfotransferase activity for the synthesis of sulfatide is induced by progesterone.
4 N-(O-linoleoyl) hydroxy fatty acyl sphingosyl glucose was found to be characteristically contained in mammalian epidermis and showed an activity enhancing the kerachinization process of kerachinocytes established from fetal rat skin. The activity involved the stimulation of cornified envelops-formation and the activation of glutamyl peptide transglutaminase and was found to be the same activity as that induced by phorbol ester.

  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Iwamori,M.,Sakayori,M.,Nozawa,S.,Yamamoto,T.,Nagai,Y.: "Monoclonal antibodyーdefined antigen of human uterine endometrium carcinoma is Le^a" Journal Biochemistry. 105. 718-722 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kubushiro,K.,Kojima,K.,Nozawa,S.,Iwamori,M.,Nagai,Y.: "Menstrual cycleーassociated alteration of sulfogalactosylceramide in human uterine endometrium;Possible induction of glycolipid sulfation by steroid hormones" Arch.Biochem.Biophys.268. 126-136 (1989)

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      「研究成果報告書概要(和文)」より
  • [Publications] Nagai,Y.,Tsuji,S.: "Biological significance of ectoーbiosignal transduction:Carbohydrate recognition coupled with cell surface protein phosphorylation" Gangliosides and Cancer. 3. 33-39 (1989)

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      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto,H.,Tsuji,S.,Nagai,Y.: "Tatrasialoganglioside GQlb reactive monoclonal antibodies:Their characterization and applicaition for quantitation of GQlb in some cell lines of neural and adrenal origins" Journal of Neurochemistry. 54. 513-517 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sanai,Y.,Oda,K.,Nagai,Y.: "Induction of GD3 ganglioside by adenovirus E1A gene 13Sー and 12SーmRNA products in rat 3Yl cells" Journal of Biochemistry. 107. 740-742 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uchida,Y.,Iwamori,M.,Nagai,Y.: "Activation of kerachinization of kerachinocytes from felal rat skin wit Nー(Oーlinoleoyl)hydroxy fatty acyl sphingosyl glucose(lipokerachinogenoside)as a marker of epidermis" Biochem.Biophys.Res.Commun.170. 162-168 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kubushiro, K., Kojima, K., Mikami, M. Nozawa, S., Iizaka, R., Iwamori, M. and Nagai, Y.: "Tumor-associated muin-type glyco-protein defined by two monoclonal antibodies in ovarian cancer" Cancer Res. 49. 493-498 (1989)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kubushiro, K., Kojima, K., Mikami, M., Nozawa, S., Iizuka, R., Iwamori, M. and Nagai, Y.: "Menstrual cycle-associated alteration of sulfoglactsylceramide in human uterine endometrium : Possible induction of glycolipid sulfation by sex steroid hormones" Arch. Biochem. Biophys.268. 126-136 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, J., Kato, T., Okumura. K., Miyatani, Y., Tanaka, R., Tsuji, S., and Nagai, Y.: "Induction of astroglial growth inhibition and differentiation by sialyl cholesterol" Brain Res.481. 335-343 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamori, M., Sakayori, M., Nozawa, S., Yamamoto, T., Yago, M., Noguchi, M. and Nagai, Y.: "Monoclonal antibody-defined antigen of human uterine endometrial carcinoma is Leb" J. Biochem. 105. 718-722 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamori, M., Kawaguchi, T. and Nagai, Y: "Differential exposure of the major gangliosides in rabbit thymocytes to vibrio cholerae neuraminidase" J. Biochem.105. 723-727 (1989)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kosugi, S., Mori, T., Iwamori, M., Nagai, Y. and Imura, H: "Adrenergic receptors and adenosine Al receptor of FRTL-5 cells in relation to fucosyl GMl ganglioside" Endocrinology. 124. 2707-2710 (1989)

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  • [Publications] Kusumoki, S., Inoue, K Iwamori, M., Nagai, Y. and Mannen, T: "Discrimination of human dorsal root ganglion cells by anti-fucosyl GMl antibody" Brain Res.494. 391-395 (1989)

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  • [Publications] Kashiwagi, M., Iketa, Y., Iwamori, M., Toda, G. and Nagai, Y.: "Significant increase in the antibody to Gall-3Gal structure in sera of patients with hepatocellular carcinoma after transcatheter embolization" Cancer Res.49. 4396-4401 (1989)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Nagai, Y. and Tsuji, S.: "Bioactive ganglionside-mediated carbohydrate recognition in coupling with ecto-protein phosphorylation Carbohydrate recognition in Cellular Function" Ciba Foundation Symposium. 119-134 (1989)

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  • [Publications] Itonori, S., Hidari, K., Sanai, Y., Taniguchi, M. and Nagai, Y.: "Involvement of the acyl chain of ceramide in carbohydrate recognition by an anti-glycolipid monoclonal antibody : The case of an anti-melanoma antibody to GM3 ganglioside" Glycoconjugate J.6. 551-560 (1989)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Nakakuma, H., Arai, M., Kawaguchi, T., Horikawa, K., Hidaka, M., Sakamoto, K., Iwamori, M., Nagai, Y. and Takatsuki, K.: "Monoclonal antibody to galactosylceramide : Discrimination of structural difference in the ceramide moiety" FEBS Lett.258. 230-232 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, H., Tsuji, S. and Nagai, Y.: "Tetrasilaoganglioside GQlb reactive monoclonal antibodies : Their characterization and application for quantification of GQlb in some cell lines of neural and adrenal origin" J. Neurochem.54. 513-517 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki-Sekimori, R., Matsuoka, K., Nagai, Y. and Takenawa, T.: "Di-acylglycerol but not inositol 1, 4, 5-triphosphate, accounts for platelet-derived growth factor-stimulated proliferation of Balb 3T3 cells" J. Cell Physiol.140. 432-438 (1989)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamura, A., Kijima-Suda, I., Sugimoto, M., Itoh, M., Takada, K., Tomita, K., Ogawa, T. and Nagai, Y.: "A monoclonal antibody to free N-acetylneuraminic acid" Biochim. Biophys. Acta. 1033. 201-206 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sanai, Y., Oda, K. and Nagai, Y.: "Induction of GD3 ganglioside by adenovirus ElA gene 13S- and 12S-mRNA products in rat 3Yl cells" J. Biochem.107. 740-742 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakakuma, H., Kawaguchi, T., Horikawa, K., Iwamori, M. and Nagai, Y.: "Altered expression of gangliosides in erythrocytes of paroxysmal nocturnal hemoglobinuria" J. Clin. Invest.85. 1456-1461 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchida, Y., Iwamori, M. and Nagai, Y.: "Activation of keratinization of keratinocytes from fetal rat skin with N-(O-linoleoyl) hydroxy fatty acylsphingosyl glucose (lipokeratinogenoside) as a marker of epidermis" Biochem. Biophys. Res. Commun.170. 162-168 (1990)

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Published: 1993-08-12  

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