1991 Fiscal Year Final Research Report Summary
Studies on the abnormality in human proTRH cDNA of patients with TRH deficiency.
| Project/Area Number |
01480284
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | Gunma University |
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Principal Investigator |
MORI Masafumi Gunma University Professor, 医学部, 教授 (80174382)
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| Project Period (FY) |
1989 – 1991
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| Keywords | proTRH / proTRH cDNA / mRNA / PCR / Hypothalamus / TRH deficiency / Depression |
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| Research Abstract |
RNAs derived from human and mouse hypothalami were reversetranscrived to each cDNA. Using each cDNA as a temprate and primers corresponding to the sequence of cDNA encoding rat proTRH, polymerase chain reaction was performed. The resulted cDNA was subcloned into pGEM3Z vector and the sequence determined. The human proTRH cDNA was transfected into CHO cells and resulted proteins were analyzed for proTRH using the specific anti-proTRH antibody. Restriction fragment length polymorphism using the human proTRH cDNA showed no significant difference in DNAs of normal control subjects and patients with TRH-deficiency and dpression.
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