1991 Fiscal Year Final Research Report Summary
Transplantation of genetically modified cultured neurons into the brain with Parkinson's disease.
| Project/Area Number |
01480361
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Keio University |
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Principal Investigator |
TOYA Shigeo Keio Univ., School of Medicine, Professor, 医学部, 教授 (40051205)
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| Co-Investigator(Kenkyū-buntansha) |
UEMURA Keiichi Keio Univ., School of Medicine, Professor, 医学部, 教授 (90049792)
ASADA Hideo Keio Univ., School of Medicine, Instructor, 医学部, 助手 (10184144)
YOSHIDA Kazunari Keio Univ., School of Medicine, Instructor, 医学部, 助手 (70166940)
TAKAYAMA Hideichi Keio Univ., School of Medicine, Instructor, 医学部, 助手 (50171559)
OTANI Mitsuhiro Keio Univ., School of Medicine, Instructor, 医学部, 助手 (80051605)
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| Project Period (FY) |
1989 – 1991
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| Keywords | Parkinson's disease / Transplantation / Cultured neuron / Nerve growth factor / Neuroblastoma / Retinoic acid / Vascularization / Rejection |
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| Research Abstract |
1. The reconstruction, the origin, and the blood-brain-barrier (BBB) of the blood vessels of mouse cortical tissue grafted into the third ventricle of rat brain were investigated. The following are the results of the study. 1) Some of the blood vessels in the graft were derived from the donor mouse tissue. 2) The host blood was supplied to the blood vessels derived from the donor. 3) BBB is existent iii the graft from 5th day after transplantation, but injured at the 14th post-operative day presumably by the immunological rejection mechanism.
2. The immunological rejection in the xenograft was sufficiently suppressed by FK-506, the product of Streptomyces tsukubaensis.
3. Human neuroblastoma cells (SH-SY5Y) were found to differentiate to mature neurons following retinoic acid treatment in vitro. The differentiated SH-SY5Y cells were grafted into the brain of Wistar albino rats, Balb/c mice, and nude inice. The grafted differentiated SH-SY5Y cells did not survive because of the immunological rejection and other reasons. However, the intravetriclar administration of NGF significantly promoted the survival of the graft. These results suggest that the cultured neurous derived from human Neuroblastoma cells could be a source of graft for Parkinson's disease.
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