1990 Fiscal Year Final Research Report Summary
Functional Structure of Centromere/Kinetochore Domain of Mammalian Chromosomes
| Project/Area Number |
01480537
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | Nagoya University |
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Principal Investigator |
OKAZAKI Tuneko Nagoya University, Department of Molecular Biology, Professor, 理学部, 教授 (10022584)
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| Co-Investigator(Kenkyū-buntansha) |
YODA Kinya Nagoya University, Department of Molecular Biology, Research Associate, 理学部, 教務員 (30126916)
MASUMOTO Hiroshi Nagoya University, Department of Molecular Biology, Assistant Professor, 理学部, 助手 (70229384)
MASUKATA Hisao Nagoya University, Department of Molecular Biology, Assistant Professor, 理学部, 助手 (00199689)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Antigenic protein recognized by ACA / CENP-B / alphoid DNA / CENP-B box / DNA-protein interaction / centromere / kinetochore |
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| Research Abstract |
The purpose of this research is to obtain the basic information reguired to understand the regulation mechanism of chromosome segregation through the identification of DNA sequence and protein factors responsible for the functional structure of centromere/kinetochore of mammalian chromosomes. Following information was obtained.
1. Highly repetitive alpha-satellite DNA (alphoid DNA) is localized at centromere domain.
2. Three antigens {CENP-A (17 kD), CENP-B (80 kD) and CENP-C (140 kD)} are detected with anticentromere antibodies in sera from autoimmune patients. These antigens are specifically associated with centromere domain of human chromosomes.
3. CENP-B and alphoid DNA form a complex at the 17 bp motif (CENP-B box) in alphoid DNA.
4. CENP-B box sequences are detected at centromere domain of all human chromosomes, including Y-chromosome in which only very small amount seems to be present. The CENP-B box is also found in centromeric satellite DNA of mouse.
5. Centromere antigens which form a complex with the CENP-B box are found in nuclear extracts of mouse, Indian muntjac and monkey cells.
6. A complex formed between alphoid DNA and CENP-B consists of two DNA molecules and probably two CENP-B molecules.
Thus, CENP-B/CENP-B box interaction is widely found in mammalian chromosomes and this interaction seems to play a role in higher order formation of centromeric alphoid DNA.
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