1990 Fiscal Year Final Research Report Summary
Studies on Trehalase Inhibitor
| Project/Area Number |
01560130
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
発酵・醸造
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| Research Institution | Kumamoto Institute of Technology |
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Principal Investigator |
MURAO Sawao Kumamoto Institute of Technology, Faculty of Engineering, Professor, 工学部, 教授 (00081472)
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| Co-Investigator(Kenkyū-buntansha) |
SHIN Takashi Kumamoto Institute of Technology, Faculty of Engineering, Associated Professor, 工学部, 助教授 (50179066)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Inhibitor / Trehalase |
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| Research Abstract |
The project was concerned on the novel trehalase inhibitor, Trehalostatin, produced by Amycolatopsis trehalostatica HG-25, which was isolated by the investigators.
The molecular formula was determined to be C_<13>H_<22>O_<10>N_2 by SIMS spectrum (m/z 367, [M+H]^+) and elemental analysis. From various spectroscopic data (13C-, 1H-, H, H-COSY, C, H-COSY, NOESY spectra), the structure was elucidated to be an pseudo-disacchaide, composed of alpha-D-glucopyranoside and 5-membered cyclitol moieties. The linkage of the two moieties was proved to be isoureido bond. This structure was a novel one, not yet reported.
Trehalostatin specifically inhibits various trehalase (microbial, blowfly, mammal), not inhibits glucoside hydrolases (alpha-, beta-glucosidase or glucoamylase).
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