1991 Fiscal Year Final Research Report Summary
Monocyte-conditioned medium enhances thrombin-stimulated PGI_2 production by human umbilical vein endothelial cells in culture
| Project/Area Number |
01570436
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Fukuoka University, School of Medicine |
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Principal Investigator |
YOSHIDA Minoru Fukuoka Univ., School of Med., Dep. of Med. Professor, 医学部・内科第二, 教授 (60078772)
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| Co-Investigator(Kenkyū-buntansha) |
ARITOMI Takamichi Fukuoka Univ., School of Med., Dep. of Med. Assistant Professor, 医学部・内科第二, 講師 (30148887)
WATANABE Kentaro Fukuoka Univ., School of Med., Dep. of Med. Assistant Professor, 医学部・内科第二, 講師 (80158625)
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| Project Period (FY) |
1989 – 1991
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| Keywords | Vascular endothelial cells / PGI_2 / Cytokines / Lipopolysaccharide / Monocytes |
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| Research Abstract |
1. HUVEC after incubation with LPS (1mug/ml), IL-1alpha (10 U/ml), IL-1beta (10 U/ml), IFN-gamma (1000 U/ml) for 24 hours produced more PGI_2 than the control HUVEC in response to thrombin. Monocyte-conditioned medium was prepared by the incubation of human peripheral blood monocytes in tissue culture flasks with (LPS-Mo-CM) or without LPS (Mo-CM). HUVEC after incubation with Mo-CM produced much more PGI_2 than LPS-, IL-1- or IFN-gamma-treated HUVEC.
2. The effect of incubation time in making Mo-CM was examined on thrombin-stimulated PGI_2 production by HUVEC treated with the Mo-CM. The longer was the incubation time in making Mo-CM, the more was PGI_2 production by HUVEC. IL-1beta in Mo-CM was also increased with the incubation time in making Mo-CM.
3. HUVEC were cultured for 24 hours with Mo-CM to which excessive-amount of anti-serum for IL-1alpha and/or TNF was added, and were then stimulated with thrombin. Consequent PGI_2 production was decreased, but still much higher than not only the control but also LPS-, IL-1- IFN-gamma-treated HUVEC. There could be produced any other cytokines from monocytes except IL-1 or TNF which stimulate PGI_2 production by HUVEC.
4. When HUVEC were cultured with Mo-CM or LPS-Mo-CM, the concentration of IL-1beta in the postculture medium of HUVEC was not significantly changed, compared with Mo-CM or LPS-Mo-CM before HUVEC were cultured with. IL-1beta did not enhance the production of IL-1beta from HUVEC.
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