1991 Fiscal Year Final Research Report Summary
Elucidation of mechanism in pripheral nerve regeneration
| Project/Area Number |
01570844
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
HIRASAWA Yasusuke Kyoto Pref. Univ. of Med. Prof., 医学部, 教授 (40079851)
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| Co-Investigator(Kenkyū-buntansha) |
KATSUMI Yasukazu Meiji Univ. of ori. Med. Prof., 鍼灸学部, 教授 (20161086)
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| Project Period (FY) |
1989 – 1991
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| Keywords | Schwann cell / Neurotrophic Factor / Laminin / Dorsal Root Genglia / Pheocheomocytoma PC12 / Nerve Growth Factor / Retina |
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| Research Abstract |
The mechanism underlying the peripheral nerve regeneration is a fundamental problem of neurobiology. Roles of Schwann cells in the nerve regeneration may be ascribe cell-cell interaction, cellmatrix interaction, and trophic molecules. Neurotrophic molecules have a profound influence on developmental events such as naturally occurring cell death, differentiation, and neurite outgrowth. Primary culture of Schwann cells were very difficult because of contamination of fibroblast and its low multification rate. So we established six Schwann cell clones by oncogene transfer. All of the clones were labeled with antibodies to the S-100 protein and the laminin, which are specific markers for Schwann cells. Fibronectin and vimentin were not detected in these cell clones, in contrast to fibroblasts as 3T3 andRat-2. conditioned medium of RSN-moswhich established from rat neonatal sciatic nerve-promoted neurite extension in not only PC12 pheochromocytoma cells but rat fetus dorsal root ganglia and retina. The activity was resistant to NGF and laminin antiserum, not absorbed by poly-L lysin.
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