1990 Fiscal Year Final Research Report Summary
Detection of Human Papillomavirus in Head and Neck Tumors using DNA-hybridization and Immunochemical Aanalysis.
| Project/Area Number |
01570988
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Chiba university. School of Medicine. |
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Principal Investigator |
SATO Kenichi Chiba university, School of Medicine, Professor., 医学部・歯科口腔外科学, 教授 (40009139)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRASAWA Hiroshi Chiba university, School of Medicine, Assistant., 医学部(第一微生物学), 助手 (00216194)
SIMIZU Bunsiti Chiba university, School of Medicine, Professor., 医学部(第一微生物学), 教授 (10072894)
KANAZAWA Harusachi Chiba university, School of Medicine, Lecturer., 医学部(歯科口腔外科学), 講師 (80169549)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Papillomavirus / head and neck tumors / DNA-hybridization / Immunochemical Analysis / サザンブロッティング法 |
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| Research Abstract |
The presence of human papillomavirus (HPV) DNA in oral, sinus, pharynx and larynx lesions of Japanese patients was studied by Southern blot hybridization under less stringent (25% formamide, 42ﾟC) and stringent (50% formamide, 42ﾟC) conditions. Two samples from 10 benign tumors and 5 out of 30 malignant tumors contained HPV DNA or HPV-related sequences. The HPV DNAs harbored in three laryngeal papillomas were HPV 11, 6 and 6 or 11, respectively. The HPV-genome DNA, as well as viral capsid antigens, was easily detected by in situ hybridization and peroxidase-antiperoxidase staining and also by Western blotting. However, neither the typical restriction pattern of HPV DNA nor viral antigen was identified in the malignant tumors, suggesting that only parts of the HPV DNA remained integrated in the host cell DNA.
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