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1991 Fiscal Year Final Research Report Summary

New Method Using Bio-Reactor for Production of Isomaltose as Food Stuff

Research Project

Project/Area Number 01860012
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionHokkaido University

Principal Investigator

CHIBA Seiya  Hokkaido University, Faculty of Agriculture, Professor, 農学部, 教授 (30001449)

Co-Investigator(Kenkyū-buntansha) KIMURA Atsuo  Hokkaido University, Faculty of Agriculture, Instructor, 農学部, 助手 (90186312)
MATSUI Hirokazu  Hokkaido University, Faculty of Agriculture, Instructor, 農学部, 助手 (90109504)
OKADA Gentaro  Shizuoka University, Faculty of Education, Professor, 教育学部, 教授 (70021904)
Project Period (FY) 1989 – 1991
KeywordsIsomalto-dextranase / Isomaltose / Bio-reactor
Research Abstract

(1) Isomalto-dextranase was purified from Arthrobacter globiformis for mass production of isomaltose.
(2) The enzyme showed to release exo-wise isomaltose from dextran, and to release endo-wise isopanose from pullulan.
(3) A single active site of the enzyme was kinetically concluded to catalyze the hydrolyses of both alpha-1, 6- and alpha-1, 4-glucosidic linkage of substrate by using isomaltotriose and panose.
(4) Conditions of acid treatment were checked toward dextrans for the production of isomaltose. Treatments of dextrans in 0.2N HCl at 95゚C for 4 hours were most suitable.
(5) When dextrans were treated in 0.2N HCl at 95゚C for 4 hours, all of alpha-1, 2-, alpha-1, 3- and alpha-1, 4-glucosidic linkages were cleaved. Hydrolysis degree of the acid-treated dextran by the enzyme indicated more 90%, resulting in isomaltose more than 70% in yeild.

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Seiya Chiba: "Anomeric Configuration of Crystallin 2ーDeoxymaltose" Agric.Biol.Chem.54. 3023-3024 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirokazu Kawagishi: "Isolation and Characterization of A lectin from Grifola frondosa Fruiting Bodies" Biochim.Biophs.Acta. 1034. 247-252 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Atsuo Kimura: "Allosteric Properties,Substrate Specificty andSubsite Affinities of Honeybee αーGlucosidase I" J・Biochem. 107. 762-768 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡田 嚴太郎: "ゲンチオオリゴ糖を主成分とする機能性苦味糖質の開発" BIO INDUSTRY. 7. 662-668 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡田 嚴太郎: "ゲンチオリゴ糖の性状と食品への応用" New Food Industry. 32. 28-32 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡田 嚴太郎: "セルラ-ゼ研究の進歩と今後の展開" バイオサイエンスとインダストリ-. 48. 1055-1062 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡田 嚴太郎: "Trichoderma virideの加水分解酵素に関する研究" 澱粉科学. 37. 287-298 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松井 博和: "アイソト-プ効果によるエキソーαーグルカナ-ゼの反応機構の解析" 澱粉科学. 38. 181-185 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirokazu Kawagishi: "A Lectin from the Mushroom aurirella" Agric.Biol.Chem.55. 2485-2489 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akihiko Kita: "Substrate Specificity and Subsite Affinities of Cystalline αーGlucosidase from Aspergillus niger" Agric.Biol.Chem.55. 2327-2335 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sumio Kitahata: "Mechanism of Maltal Hydration Catalyzed by βーAmylase:Role of Protein Structure in Controlling the Steric Outcome of Reaction Catalyzed by Glycosylase" Biochemistry. 30. 6769-6775 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wolfgang Weiser: "Stereochemistry of DーGalactal and DーGalactoーoctenitol Hydration by Coffee Bean αーGalactosidase:Insignt into Catalytic Functioning of the Enzyme" Arch.Biochem.Biophys.292. 493-498 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiya Chiba: "Anomeric Configuration of Crystalline 2-Deoxymaltose" Agric. Biol. Chem.54. 3023-3024 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirokazu Kawagishi: "Isolation and Characterization of A lectin from Grifola frondosa Fruiting Bodies" Biochim. Biophys. Acta. 1034. 247-252 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Atsuo Kimura: "Allosteric Properties, Substrate Specificity and Subsite Affinities of Honeybee alpha-Glucosidase I" J. Biochem.107. 762-768 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gentaro Okada: "Development of Gentiooligosaccharide as Bitler Material" BIO INDUSTRY. 7. 662-668 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gentaro Okada: "Utilization of Gentiooligosaccharide to Food" New Food Industry. 32. 28-32 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gentaro Okada: "Advances in Studies on Cellulase" Bioscience and Industry. 48. 1055-1062 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gentaro Okada: "Studies on the Hydrolases from Trichoderma viride" Denpun Kagaku. 37. 287-298 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirokazu Matsui: "alpha-Secondary Isotope Effects in Reaction of Exo-alpha-Glucanases" Denpun Kagaku. 38. 181-185 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirokazu Kawagishi: "A Lecitin from the Mushroom aurirella" Agric. Biol. Chem.55. 2485-2489 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akihiko Kita: "Substrate Specificity and Subsite Affinities of Crystalline alpha-Glucosidase from Aspergillus niger" Agric. Biol. Chem.55. 2327-2335 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sumio Kitahata: "Mechanism of Maltal Hydration Catalyzed by beta-Amylase : Role of Protein Structure in Controlling the Steric Outcome of Reaction Catalyzed by a Glycosylase" Biochemistry. 30. 6769-6775 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wolfgang Weiser: "Stereochemistry of D-Galactal and D-Galacto-octenitol Hydration by Coffee Bean alpha-Galactosidase : Insight into Catalytic Functioning of the Enzyme" Arch. Biochem. Biophys.292. 493-498 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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