1991 Fiscal Year Final Research Report Summary
Reactivation of proteins and membrane transport by the heat shock proteins from thermophilic bacteria.
| Project/Area Number |
01870015
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Jichi Medical School |
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Principal Investigator |
KAGAWA Yasuo Jichi Medical School, Facul. Med., Professor, 医学部, 教授 (30048962)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANISHI Makoto Jichi Medical School, Facult. Med., Assistant, 医学部, 助手 (40217774)
OHTA Shigeo Jichi Medical School, Facult. Med., Assoc. Prof., 医学部, 助教授 (00125832)
HAMAMOTO Toshiro Jichi Medical School, Facult. Med., Lecturer, 医学部, 講師 (30189625)
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| Project Period (FY) |
1989 – 1991
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| Keywords | heat shock protein / thermophilic chaperonin / reconstitution / GroEL / thermophilic gene / hsp gene / ATP synthase / oligomer |
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| Research Abstract |
Heat shock proteins are essential for tlie formation and restoration of many supramolecular structures. For this purpose, we isolated stable heat shock proteins of 6lkDa, 12kDa and 69kDa, which are similar to GroEL, GroES and DnaK, respectively, 'from the supernatant fraction of thermophilic bacterium PS3 by ATP-Agarose chromatography. Using synthetic DNA of the deduced sequence, the 1.6kbp DNA encoding both proteins was obtained by polymerase chain reaction. The complete sequence of the resulting reading frames showed high. homology to those of the genes encoding GroEL and GroES, and the 12kDa and 6lkDa genes were present in the same operon. 61KDa was also partially similar to the F_1alpha subunit of thermophilic ATP synthase. Using the over-expressed thermophilic F_1alpha subunit it was possible to isolate t
he alpha_3beta_3 and alpha_1beta_1 subunit complexes. These results subunits have been analyzed by synchrotron radiation etc. These results are precious to analyze energy transformation in the reconstituted membrane system.
Patent : Toku-Gan-Hei#2-73664, Heat shock protein, Applicant : Yasuo Kagawa.
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[Publications] Tamada,H.,Ohta,T.,Hamamoto,T.,Otawara-Hamamoto,Y.,Yanagi,M.,Kagawa,Y.: "Gene structure of heat shock proteins 61KDa and 12KDa (thermphilic chaperonins) of thermophilic bacterium PS3." Biochem.Biophys.Res.Commun. 179. 565-571 (1991)
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「研究成果報告書概要(和文)」より
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[Publications] Kagawa,Y.,Ohta,S.,Hamamoto,T.,Harada,M Ito,Y.and Sato,M.: "New Era of Bioenergetics.The alphal betal heterodimer and molecular assembly of ATP synthase" Academic Press,New York,(Mukohata,Y.ed.), 560 (1991)
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「研究成果報告書概要(和文)」より
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[Publications] Tamada, H., Ohta, T., Hamamoto, T., Otawara - Hamamoto, Y., Yanagi, M., Hiraiwa, H., Hirata, H. and Kagawaw, Y.: "Gene structure of heat shock proteins 61KDa and 12KDa (thermophilic chaperonins) of thermophilic bacterium PS3." Biochemical and Biophysical Research Communications. 179(1). 565-571 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Harada, M., Ito, Y., Sato, M., Aono, O., Ohta, S., and Kagawa, Y.: "Small - angle X - ray scattering studies of Mg・AT (D) P - induced hexamer to dimer dissociation in the reconstituted alpha3 beta3 complex of ATP synthase from thermophilic bacterium PS3." Journal of Biological Chemistry. 266. 11455-11460 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Kagawa, Y., Ohta, S., Hamamoto, T., Harada, M. Ito, Y., and Sato, M.: Academic Press, N. Y.New Era of Bioenergetics. (Mukohata, Y. ef.)(The alpha1 beta1 heterodimer and molecular assembly of ATP synthase.), (1991)
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「研究成果報告書概要(欧文)」より
