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1991 Fiscal Year Final Research Report Summary

Developent of Methodology to Assess Viability of Embryos

Research Project

Project/Area Number 01870071
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionUniversity of Tokyo

Principal Investigator

MIZUNO Masahiko  University of Tokyo, Department of Obstetrics and Gynecology, Professor, 医学部(病), 教授 (10010175)

Co-Investigator(Kenkyū-buntansha) KATO Takahiko  Yokohama City University Department of Biochemistry, Professor, 医学部, 教授 (80010023)
HIROI Masahiko  University of Yamagata, Department of Obstetrics and Gynecology, Professor, 医学部, 教授 (60018364)
TSUTSUMI Osamu  University of Tokyo, Department of Obstetrics and Gynecology, Assistant, 医学部(病), 助手 (60134574)
Project Period (FY) 1989 – 1991
Keywordsoocyte / embryo / in vitro fertilization / glucose / hexokinase / epidermal growth factor / glucose transporter / glucose uptake
Research Abstract

In the course of work involving mammalian embryo culture and transfer, workers must make judgements regarding embryo quality or viability. At present, assessments of embryo viability are usually made on the basis of morphological integrity. Visual assessment of embryos is subjective and there is a great deal of variation within groups of morphologically sound embryos in terms of viability. It would be useful if quantitative tests were available to assess embryo viability prior to transfer or after some experimental treatment. We have developed microdetermination methods which can be applied to single mammalian oocyte such as mouse and human.
Pyruvate is an essential nutrient until the 8-cell at age embryos and glucose will serve as an energy source thereafter. To analyze the characteristics of energy metabolism of oocytes and embryos, we deviced micro-determination methods to quantify enzyme activities and glucose uptake of them. Hexokinase activites showed a continuous and exponetial increase during their development. The increasing speed of hexokinase activity exceeded the doubling speed in the cell number after the 8-cell stage. Deoxyglucose, an analogue of glucose, incorpor-ation was also low in unfertilized oocytes. Fertilization lead to a salutatory rise of the deoxyglucose incorporation activity and the activity rapidly increased during preimplantation development. These may serve as indicators of quality or viability of oocytes and embryos.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Masahiko Mizuno et al.: "Role of prostaglandins in egg implantation." Adv.Gynecol.Endocrinol.1. 433-439 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Tsutsumi et al.: "Studies of hexokinase activity in human and mouse oocyte." Am.J.Obstet.Gynecol.1301-1304 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsong-Ming Kuo et al.: "Stimulatory effect of epidermal growth factor on the developmentof mouse early embryo in vitro." Endocrinol.Japon.38. 485-490 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 堤 治他: "卵成熟機構の生化学的解析" 日本受精着床学会雑誌. 8. 14-17 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 成 鑑非他: "体外受精時卵胞液に検出されるhCG濃度と卵成熟の関連" 日本産科婦人科学会雑誌. 43. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 細谷 岩生他: "微小培養法を用いた単一卵分析によるマウス初期胚糖取込み能発達の解析" 日本産科婦人科学会雑誌. 43. 1363-1366 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 堤 治,水野 正彦: "助産学講座2生殖の形態・機能「卵巣機能」" 医学書院我妻尭、前原澄子編, (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤 尚彦: "神経生化学「酵素的サイクリング」" 日本生化学会篇東京化学同人, (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Tsutsumi et al.: "Studies of hexokinase activity in human and mouse oocyts." Am. J. Obstet. Gynecol.162. 1301-1304 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriyasu Saito et al.: "Estrogen-binding proein in blood and mfollicular fluid and its biochemical properties in human females." Gynecol. Obstet. Invest.28. 87-93 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jun-ichi Takagi et al.: "The development of porcine zona pellucida using monocronal antibodies : I. Immunochemistry and light microscopy" Biol. Reprod.40. 1095-1102 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira Akabayashi et al.: "One-step and two step fluorometric assay methods for general aminotransferases using glutamate dehydrogenase." Anal. Biochem.182. 129-135 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira Akabayashi et al.: "An enzymatic microassay method for deoxyglucose and deoxyglucose 6-phosphate." Biomedical Research. 10. 173-177 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinnousuke Kawagoe et al.: "Involvement of prolactin in the control of follicular maturation." Gynecol. Obstet. Invest.27. 197-200 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Mizuno et al.: "Role of prostaglandins in egg implantation." Adv. Gynecol. Obstet.1. 433-439 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tson-Ming Kuo et al.: "Stimulatory effect of epidermal growth factor on the development of mouse early embryo in vitro." Endocrinol. Japon.38. 485-490 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akabayashi et al.: "An enzymatic microdetermination method for hydroxylase cofactor." Anal. Lett.24. 1967-1981 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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