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1991 Fiscal Year Final Research Report Summary

Technical Development for Making the Artificial Dental Root with Periodontal Ligament

Research Project

Project/Area Number 01870079
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionTohoku University

Principal Investigator

OKUDA Reiichi  Tohoku University, Dentistry, Professor, 歯学部, 教授 (80005024)

Co-Investigator(Kenkyū-buntansha) TAGAMI Atsushi  Tohoku University, Dental Hospital, Assistant Professor, 歯学部附属病院, 助手 (00236374)
SHIMIZU Yoshinobu  Tohoku University, Dentistry, Associate Professor, 歯学部, 助教授 (20005078)
KAGAYAMA Manabu  Tohoku University, Dentistry, Professor, 歯学部, 教授 (60004610)
Project Period (FY) 1989 – 1991
KeywordsArtificial Dental Root / Pure Titanium / Periodontal Ligament Cells / Cell Culture / Suppression of Bone Resorption / Alkaline Phosphatase
Research Abstract

The purpose of this study is the establishment of artificial dental root, which is functionally close to the natural teeth. In the process of the developing technique to make periodontal ligament (PDL) on the surface of titanium artificial root, we investigated the character of PDL cells invitro or in vivo. The main results observed were as follows.
1. The primary cultivation of human PDL cells could be obtained at the rate of success 90% or more with our improved method. 2. Human PDL cultured cells could be adsorbed on the titanium surface. 3. Cell-Tak coating could accelerate the absorbance of human PDL cells onto titanium surface. 4. The growth velocity of PDL cells was the most accelerated in the medium supplemented with 10% human serum. 5. The polished and glass beads-sandblasted titanium surface were appropriate for the growth of PDL cells. 6. The factor produced from PDL cells could suppress the induction of osteoclast from the cultured peripheral blood lymphocytes and monocytes (PBL). 7. Gingival cells and osteoblast originated from alveolar bone didn't produce this suppressing factor. 8. Although number of osteoclast induced from the PBL could be increased by the stimulation of some bacteria from periodontal lesion, the addition of the culture fluid of PDL cells significantly decreased the induction of osteoclast from PBL stimulated with bacterias. 9. ALPase activity of PDL cells was not suppressed by co-culture with PBL. 10. As compared with gingival cells, ALPase activity and collagen synthesis of PDL cells was remarkably higher. 11.10th Passage of cells did not show in any defective effect on differentiating function of PDL cells.
Concerning casting system of titanium, development on the investment and the technical improvement on design of sprue and vent were also attained.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 斎藤 素子: "歯根修復に関する研究 第2報 各種形状純チタン面へのヒト歯根膜細胞培養と機能発現" 日本歯科保存学雑誌. 32. 1612-1621 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水 義信: "人工歯根修復に関する模索" 歯科ジャ-ナル. 31. 477-486 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田上 篤: "歯根修復に関する研究 第3 報歯根膜由来細胞にみられる骨吸収メカニズム抑制作用" 歯科ジャ-ナル. 33. 275-283 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊藤 慎一: "歯根膜細胞による破骨細胞の誘導形成抑制ー菌体刺激による末梢血リンパ球からの破骨細胞形成に対する歯根膜細胞の作用ー" 日本歯科保存学雑誌. 34. 1485-1497 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡部 太一: "歯根修復に関する研究 第5 報年齢別及び細胞継代数によるヒト歯根膜細胞の分化機能の比較" 東北大学歯学雑誌. 10. 87-99 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水 義信: "歯根修復に関する研究 第4 報歯科インプラントと歯根膜" 歯界展望. 79. 479-486 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reiichi Okuda: "Assessment of an Experimental Investment Specified for titanium Casting by the Observation of the Castings" Dentistry in Japan. 28. 1201-1213 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Motoko Saito: "Studies on Root Restoration with Titanium Blade Part2 Cell Culture of Human PDL Cells on Titanium with Various Micro surface stracture" Japan. J. Conserv. Dent.32-6. 1612-1621 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshinobu Shimizu: "Quest for a Good Artificial Root Restoration Form" The Journal of Dental Medicine (Japan). 31-4. 477-486 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Atsushi Tagami: "Studies on Root Restoration with Titanium Blade Part3 Resistant Activity of Periodontal Ligament Cells onto Bone Resorbing Mechanisms" The Journal of Dental Medicine (Japan). 33-2. 275-283 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinichi Itoh: "Suppression of Osteoclast Formation by Periodontal Ligament Cells -Influence of Periodontal Ligament Cells onto Osteoclast Formation from Peripheral Blood Lymphocytes Stimulated by Bacterias-" Japan. J. Conserv. Dent.34-5. 1485-1497 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taichi Okabe: "Studies on Root Restoration Part5 Effect of passage on differentiating function of human periodontal ligament cells derived from various aged patients" Tohoku Univ. Dent. J.10-2. 87-99 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshinobu Shimizu: "Studies on Root Restoration Part4 Dental Inplants with Periodontal Ligament" Dental Outlook. 79-2. 479-486 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Reiichi Okuda: "Assessment of an Experimental Investment Specified for Titanium Casting by the Observation of the Castings" Dentistry in Japan. 28. 1201-1213 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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