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1991 Fiscal Year Final Research Report Summary

Molecular polymorphism, gene regulation and adaptive evolution of duplicated amylase genes in Drosophila.

Research Project

Project/Area Number 02044113
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionKyusyu University

Principal Investigator

YAMAZAKI Tsuneyuki  Kyushu University, Faculty of Science, Professor, 理学部, 教授 (10108649)

Co-Investigator(Kenkyū-buntansha) WINIFRED Doane  Arizona State University, Professor, 教授
HICKEY D.  オタワ大学, 教授
CHUCK Langley  California University, Professor, 教授
PHIL Hedrick  Pennsylvania State University, Professor, 教授
LEWONTIN R.C  ハーバード大学, 教授
D. Hickey  Ottawa University, Professor
R. C. Lewontin  Harvard University, Professor
Project Period (FY) 1990 – 1991
KeywordsIsozyme polymorphism / Amylase / Duplicated genes / Concerted evolution / Drosophila / gene regulation / Inducibility
Research Abstract

DNA sequences of several polymorphic isozymes at duplicated amylase genes were determined in Drosophila melanogaster and its sibling species. Large differences in base sequences between different allozymes compared with identical allozymes indicated long history of different isozymes. In addition, differences between the two duplicated amylase genes within species were much smaller than those of the equivalent locus between different species, indicating the existence of concerted evolution between duplicated genes within species the evidence of concerted evolution was observed only in the coding region but not in the spacer regions. Theoretical study is still under way, concerning the molecular mechanism of this phenomenon. We also cloned a transfacting regulatory gene on the X-chromosome, which appears to affect the inducibility of amylase. The function of this gene is being investigated using P-element mediated transformation technique.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Mukai,T.,K.Harada,Kusakabe and T.Yamazaki: "Estimation of Spontaneous mutation rates at enzyme loci in Drosophila melanogaster." Proc.Natl.Acad.Sci.USA. 66. 29-32 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nitasaka,E.and T.Yamazaki: "A novel eye morphology induced by P element in somatic tissue of Drosophila melanogaster." Molecular and General Genetics. 227. 181-186 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nitasaka,E.and T.Yamazaki: "The relationship between DNA structural variations and activities of P elements in typical P and Q strains of Drosophila melanogaster." Genet.Res.Camb.(1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nitasaka,E.,M.M.Green,and T.Yamazaki: "The molecular analysis of brown eye color mutations isolated from geographically discrete populations of Drosophila melanogaster." Proc.Natl.Acad.Sci.USA. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hawkley,S.A.,W.W.Doane and R.A.Norman: "Molecular analysis of cls-regulatory sequenses at the α-amylase locus in Drosophila melanogaster." Genetics. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Doane,W.W.,D.S.Thompson R.A.Norman & S.A.Hawkley: "Molecular genetics of a three-gene cluster in the Amy region of Drosophila." Isozymes. 344. 19-48 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamazaki,T.: "POPULATION BIOLOGY OF GENES AND MOLECULES (Nucleotide polymorphism and evolution of multigine families in Drosophila.)" ed.by N.Takahata and J.F.Crow (Baifukan), 151-167 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nitasaka,E.,and T.Yamazaki: "NEW ASPECT OF GENETICS IN MOLECULAR EVOLUTION (Genetic variation and regulation of P elements in Drosophila melanogaster.)" ed.by Kumura,M.and T.Takahata (japan Sci.Press), 1-14 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mukai, T., K. Harada, Kusakabe and T. Yamazaki: "Estimation of Spontaneous mutation rates at enzyme loci in Drosophila melanogaster." Proc. Natl. Acad. Sic. USA.66. 29-32 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nitasaka, E. and T. Yamazaki: "A novel eye morphology induced by P element in somatic tissue of Drosophila melanogaster." Molecular and General Genetics.227. 181-186 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nitasaka, E., and T. Yamazaki.: "The relationship between DNA structural variations and activities of P elements in typical P and Q strains of Drosophila melanogaster." Genet. Res. Camb.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nitasaka, E., M. M. Green and T. Yamazaki: "The molecular analysis of brown eye color mutations isolated from geographically discrete populations of Drosophila melanogaster." Proc. Natl. Acad. Sci. USA.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hawkley, S. A., W. W. Doane and R. A. Norman: "Molecular analysis of cls-regulatory sequences at the alpha-amylase locus in Drosophila melanogaster." Genetics. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamazaki, T.: N. Takahata and J. F. Crow, Baifukan. Population biology of genes and molecules., 151-167 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nitasaka E. and T. Yamazaki: Kimura, M. and T. Takahata, Japan Sci. Press.New aspect of genetics in molecular evolution, 1-14 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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