1990 Fiscal Year Annual Research Report

植物遺伝子発現の転写制御シグナルと高次調節機構

Research Project

Project/Area Number	02304006
Research Institution	Kyoto University
Principal Investigator	岩渕雅樹京都大学, 理学部, 教授 (30000839)
Co-Investigator(Kenkyū-buntansha)	山崎健一名古屋大学, 農学部, 助手 (40182480) 内宮博文北海道大学, 理学部, 助教授 (50142229) 町田泰則名古屋大学, 理学部, 教授 (80175596) 中村研三名古屋大学, 農学部, 助教授 (80164292) 南川隆雄東京都立大学, 理学部, 教授 (30087001)
Keywords	植物遺伝子転写 / シス・エレメント / トランス制御因子 / 高次調節機構 / 細胞特異的遺伝子発現 / DNA結合核タンパク質
Research Abstract	各研究担当者がこれまで取り扱って来ている植物の遺伝子について,その転写制御に関わるシスエレメントとそれに特異的に結合する核タンパク質の研究を班員相互に技術・情報交換を積極的に行ないつつ推進した結果,初年度において以下のような成果をあげることができた。ナタマメのカナバリン遺伝子について,主要なシス領域はー745までに存在すること,及びその下流に結合するタンパク質を同定した(南川)。サツマイモのスポラミンおよびβーアミラ-ゼ遺伝子の5'上流域に共通に結合する核タンパク質SP8を見出した。また,後者の遺伝子のー800域に結合するタンパク質はコムギ転写因子HBPー1bと類似のものであることが示された(中村)。オ-キシンで発現が促進されるタバコpar遺伝子のー1679までの中に,この遺伝子が根毛,根端及び登熟過程の種子で特異的に発現するシス領域が存在することが示された。さらにこのシス領域には発現を正と負に制御するエレメントも存在した。また,ー50〜ー100にはタバコ転写因子TGAー1aが結合した(町田)。コムギ・ヒストンH3遺伝子の転写の細胞周期のS期特異性を規定するシス領域がおおよそ同定された。さらに,そのシス領域のうちのヘキサマ-配列に結合するタンパク質HBPー1a及びHBPー1bの構造と機能を両因子のcDNAを用いて解析し,それらが転写制御因子であることを明らかにした(岩渕)。また,両因子の遺伝子のクロ-ン化にも成功し,その構造解析も進行中である(三上)。RiプラスミドのrolC遺伝子のプロモ-タ-領域に結合するタンパク質の多様性が示された(内宮)。また,エンドウ葉緑体遺伝子の転写因子(zfpA)がzinc finger構造を持つことが明らかとなった(佐々木)。CaMV遺伝子プロモ-タ-の小麦胚芽抽出液を用いた転写系において,鋳型が超らせん構造の時,高い転写活性を示した(山崎)。細胞性粘菌の細胞分化における胞子細胞特異的遺伝子(Dp87,Sp96)のシス領域とDNaseI感受性領域を決定した(竹内)。

Research Products

(23 results)

All Other

All Publications (23 results)

[Publications] Kawata,Takefumi: "Cell cycleーregulated gene expression in transgenic plant cells" Develop.Genet.11. 205-213 (1990)
[Publications] Tabata,Tetsuya: "HBPー1a and HBPー1b:Leucineーzipper type transcription factors of wheat" EMBO J.(1991)
[Publications] Mikami,Koji: "Regulation of cell cycleーdependent gene expression" In“Control of Plant Gene Expression"(ed.D.P.S.Verma),Telford Press. (1991)
[Publications] Mikami,Koji: "Cisーacting elements and transーacting factors in the transcription of higher plant histone genes" Oxford Survey of Plant Molecular and Cell Biology. (1991)
[Publications] Yamauchi,D.: "Structure of the gene encoding concanavalin A from <Canavalia>___ー <gladiata>___ー and its expression in <Escherichia>___ー <coli>___ー cells" FEBS Lett.260. 127-130 (1990)
[Publications] Akasofu,H.: "Nucleotide sequence of the gene for sulfhydrylーendoーpeptidase (SHーEP) from <Vigna>___ー <mungo>___ー" Nucleic Acids Res.18. 1892 (1990)
[Publications] Kanaya,K.: "Specific binding of nuclear protein from tobacco hairy roots cultured in vitro to a 5'ーupstream region of the rolC gene of the Ri plasmid" Plant Cell Physiol.31. 941-946 (1990)
[Publications] Fujii,N.: "Conditions favorable for the somatic embryogenesis in carrot cell culture enhance expression of the <rolC>___ー promoterーGUS fusion gene" Plant Physiol.95. 238-241 (1991)
[Publications] Hattori,T.: "Sucroseーinduced expression of genes coding for tuberous root storage protein,sporamin,of sweet potato in leaves and petioles" Plant Cell Physiol.32. 79-86 (1991)
[Publications] Ohta,S.: "Stemーpredominant expression of the sweet potato sporamin gene promoter:βーglucuronidase (GUS) fusion gene in transgenic tobacco is conferred by multiple cell typeーspecific regulatory elements" Mol.Gen.Genet.(1991)
[Publications] Shimoda,Nobuyoshi: "Control of expression of Agrobacterium tumefaciens vir genes by synergistic actions of phenolic signal and monosaccharides" Proc.Natl.Acad.Sci.USA. 87. 6684-6688 (1990)
[Publications] Matsumoto,Shogo: "Integration of Agrobacterium TーDNA into a tobacco chromosome:Possible involvement of DNA homology between TーDNA and plant DNA" Mol.Gen.Genet.224. 309-316 (1990)
[Publications] Tasaka,Masao: "Isolation and characterization of spore coat protein (Sp96) gene of <Dictyostelium>___ー <discoideum>___ー" Cell Diff.Develop.31. 1-9 (1990)
[Publications] Sasaki,Yukiko: "Correlation of plastid DNA copy number with plastid gene expression in various organs in mature pea plants(<Pisum>___ー <sativum>___ー L.)" Plant Cell Physiol.31. 925-931 (1990)
[Publications] Nagano,Yukio: "An essential gene of <Escherichia>___ー <coli>___ー that has sequence similarity to an unknown chloroplast gene" Mol.Gen.Genet.(1991)
[Publications] Yamazaki,Kenーichi: "TGAla,a tobacco DNAーbinding protein,increases the rate of initiation in a plant <in>___ー <vitro>___ー transcription system" Proc.Natl.Acad.Sci.USA. 87. 7035-7039 (1990)
[Publications] Katagiri,F.: "A plant DNAーbinding protein increases the number of active preinitiation complexes in a human <in>___ー <vitro>___ー transcription system" Genes Develop.4. 1899-1909 (1990)
[Publications] 岩渕雅樹(分担執筆): "発生・分化の遺伝子的背景(江口・鈴木・名取編)" 東京大学出版会, 288 (1990)
[Publications] 岩渕雅樹(分担執筆): "高等植物の情報発現と制御(岡田・池田編)" 丸善株式会社, 292 (1990)
[Publications] 岩渕雅樹(分担執筆): "遺伝子の発現と制御II(志村・村松・鈴木・近藤編)" 丸善株式会社, 325 (1990)
[Publications] 岩渕雅樹(分担執筆): "植物全能性の分子生物学(駒嶺他8名編)" 朝倉書店, 305 (1991)
[Publications] 内宮博文: "植物遺伝子操作マニュアルートランスジェニック植物の作り方ー" 講談社サイエンティフィク, 138 (1990)
[Publications] 町田泰則(分担執筆): "高等植物の情報発現と制御(岡田・池田編)" 丸善株式会社, 292 (1990)

1990 Fiscal Year Annual Research Report

植物遺伝子発現の転写制御シグナルと高次調節機構

Principal Investigator

岩渕雅樹京都大学, 理学部, 教授 (30000839)

Research Products

[Publications] Kawata,Takefumi: "Cell cycleーregulated gene expression in transgenic plant cells" Develop.Genet.11. 205-213 (1990)

[Publications] Tabata,Tetsuya: "HBPー1a and HBPー1b:Leucineーzipper type transcription factors of wheat" EMBO J.(1991)

[Publications] Mikami,Koji: "Regulation of cell cycleーdependent gene expression" In“Control of Plant Gene Expression"(ed.D.P.S.Verma),Telford Press. (1991)

[Publications] Mikami,Koji: "Cisーacting elements and transーacting factors in the transcription of higher plant histone genes" Oxford Survey of Plant Molecular and Cell Biology. (1991)

[Publications] Yamauchi,D.: "Structure of the gene encoding concanavalin A from <Canavalia>_ー <gladiata>_ー and its expression in <Escherichia>_ー <coli>_ー cells" FEBS Lett.260. 127-130 (1990)

[Publications] Akasofu,H.: "Nucleotide sequence of the gene for sulfhydrylーendoーpeptidase (SHーEP) from <Vigna>_ー <mungo>_ー" Nucleic Acids Res.18. 1892 (1990)

[Publications] Kanaya,K.: "Specific binding of nuclear protein from tobacco hairy roots cultured in vitro to a 5'ーupstream region of the rolC gene of the Ri plasmid" Plant Cell Physiol.31. 941-946 (1990)

[Publications] Fujii,N.: "Conditions favorable for the somatic embryogenesis in carrot cell culture enhance expression of the <rolC>___ー promoterーGUS fusion gene" Plant Physiol.95. 238-241 (1991)

[Publications] Hattori,T.: "Sucroseーinduced expression of genes coding for tuberous root storage protein,sporamin,of sweet potato in leaves and petioles" Plant Cell Physiol.32. 79-86 (1991)

[Publications] Ohta,S.: "Stemーpredominant expression of the sweet potato sporamin gene promoter:βーglucuronidase (GUS) fusion gene in transgenic tobacco is conferred by multiple cell typeーspecific regulatory elements" Mol.Gen.Genet.(1991)

[Publications] Shimoda,Nobuyoshi: "Control of expression of Agrobacterium tumefaciens vir genes by synergistic actions of phenolic signal and monosaccharides" Proc.Natl.Acad.Sci.USA. 87. 6684-6688 (1990)

[Publications] Matsumoto,Shogo: "Integration of Agrobacterium TーDNA into a tobacco chromosome:Possible involvement of DNA homology between TーDNA and plant DNA" Mol.Gen.Genet.224. 309-316 (1990)

[Publications] Tasaka,Masao: "Isolation and characterization of spore coat protein (Sp96) gene of <Dictyostelium>_ー <discoideum>_ー" Cell Diff.Develop.31. 1-9 (1990)

[Publications] Sasaki,Yukiko: "Correlation of plastid DNA copy number with plastid gene expression in various organs in mature pea plants(<Pisum>_ー <sativum>_ー L.)" Plant Cell Physiol.31. 925-931 (1990)

[Publications] Nagano,Yukio: "An essential gene of <Escherichia>_ー <coli>_ー that has sequence similarity to an unknown chloroplast gene" Mol.Gen.Genet.(1991)

[Publications] Yamazaki,Kenーichi: "TGAla,a tobacco DNAーbinding protein,increases the rate of initiation in a plant <in>_ー <vitro>_ー transcription system" Proc.Natl.Acad.Sci.USA. 87. 7035-7039 (1990)

[Publications] Katagiri,F.: "A plant DNAーbinding protein increases the number of active preinitiation complexes in a human <in>_ー <vitro>_ー transcription system" Genes Develop.4. 1899-1909 (1990)

[Publications] 岩渕雅樹(分担執筆): "発生・分化の遺伝子的背景(江口・鈴木・名取編)" 東京大学出版会, 288 (1990)

[Publications] 岩渕雅樹(分担執筆): "高等植物の情報発現と制御(岡田・池田編)" 丸善株式会社, 292 (1990)

[Publications] 岩渕雅樹(分担執筆): "遺伝子の発現と制御II(志村・村松・鈴木・近藤編)" 丸善株式会社, 325 (1990)

[Publications] 岩渕雅樹(分担執筆): "植物全能性の分子生物学(駒嶺他8名編)" 朝倉書店, 305 (1991)

[Publications] 内宮博文: "植物遺伝子操作マニュアルートランスジェニック植物の作り方ー" 講談社サイエンティフィク, 138 (1990)

[Publications] 町田泰則(分担執筆): "高等植物の情報発現と制御(岡田・池田編)" 丸善株式会社, 292 (1990)

1990 Fiscal Year Annual Research Report

植物遺伝子発現の転写制御シグナルと高次調節機構

Principal Investigator

岩渕 雅樹 京都大学, 理学部, 教授 (30000839)

Research Products

[Publications] Kawata,Takefumi: "Cell cycleーregulated gene expression in transgenic plant cells" Develop.Genet.11. 205-213 (1990)

[Publications] Tabata,Tetsuya: "HBPー1a and HBPー1b:Leucineーzipper type transcription factors of wheat" EMBO J.(1991)

[Publications] Mikami,Koji: "Regulation of cell cycleーdependent gene expression" In“Control of Plant Gene Expression"(ed.D.P.S.Verma),Telford Press. (1991)

[Publications] Mikami,Koji: "Cisーacting elements and transーacting factors in the transcription of higher plant histone genes" Oxford Survey of Plant Molecular and Cell Biology. (1991)

[Publications] Yamauchi,D.: "Structure of the gene encoding concanavalin A from <Canavalia>___ー <gladiata>___ー and its expression in <Escherichia>___ー <coli>___ー cells" FEBS Lett.260. 127-130 (1990)

[Publications] Akasofu,H.: "Nucleotide sequence of the gene for sulfhydrylーendoーpeptidase (SHーEP) from <Vigna>___ー <mungo>___ー" Nucleic Acids Res.18. 1892 (1990)

[Publications] Kanaya,K.: "Specific binding of nuclear protein from tobacco hairy roots cultured in vitro to a 5'ーupstream region of the rolC gene of the Ri plasmid" Plant Cell Physiol.31. 941-946 (1990)

[Publications] Fujii,N.: "Conditions favorable for the somatic embryogenesis in carrot cell culture enhance expression of the <rolC>___ー promoterーGUS fusion gene" Plant Physiol.95. 238-241 (1991)

[Publications] Hattori,T.: "Sucroseーinduced expression of genes coding for tuberous root storage protein,sporamin,of sweet potato in leaves and petioles" Plant Cell Physiol.32. 79-86 (1991)

[Publications] Ohta,S.: "Stemーpredominant expression of the sweet potato sporamin gene promoter:βーglucuronidase (GUS) fusion gene in transgenic tobacco is conferred by multiple cell typeーspecific regulatory elements" Mol.Gen.Genet.(1991)

[Publications] Shimoda,Nobuyoshi: "Control of expression of Agrobacterium tumefaciens vir genes by synergistic actions of phenolic signal and monosaccharides" Proc.Natl.Acad.Sci.USA. 87. 6684-6688 (1990)

[Publications] Matsumoto,Shogo: "Integration of Agrobacterium TーDNA into a tobacco chromosome:Possible involvement of DNA homology between TーDNA and plant DNA" Mol.Gen.Genet.224. 309-316 (1990)

[Publications] Tasaka,Masao: "Isolation and characterization of spore coat protein (Sp96) gene of <Dictyostelium>___ー <discoideum>___ー" Cell Diff.Develop.31. 1-9 (1990)

[Publications] Sasaki,Yukiko: "Correlation of plastid DNA copy number with plastid gene expression in various organs in mature pea plants(<Pisum>___ー <sativum>___ー L.)" Plant Cell Physiol.31. 925-931 (1990)

[Publications] Nagano,Yukio: "An essential gene of <Escherichia>___ー <coli>___ー that has sequence similarity to an unknown chloroplast gene" Mol.Gen.Genet.(1991)

[Publications] Yamazaki,Kenーichi: "TGAla,a tobacco DNAーbinding protein,increases the rate of initiation in a plant <in>___ー <vitro>___ー transcription system" Proc.Natl.Acad.Sci.USA. 87. 7035-7039 (1990)

[Publications] Katagiri,F.: "A plant DNAーbinding protein increases the number of active preinitiation complexes in a human <in>___ー <vitro>___ー transcription system" Genes Develop.4. 1899-1909 (1990)

[Publications] 岩渕 雅樹(分担執筆): "発生・分化の遺伝子的背景(江口・鈴木・名取編)" 東京大学出版会, 288 (1990)

[Publications] 岩渕 雅樹(分担執筆): "高等植物の情報発現と制御(岡田・池田編)" 丸善株式会社, 292 (1990)

[Publications] 岩渕 雅樹(分担執筆): "遺伝子の発現と制御II(志村・村松・鈴木・近藤編)" 丸善株式会社, 325 (1990)

[Publications] 岩渕 雅樹(分担執筆): "植物全能性の分子生物学(駒嶺他8名編)" 朝倉書店, 305 (1991)

[Publications] 内宮 博文: "植物遺伝子操作マニュアルートランスジェニック植物の作り方ー" 講談社サイエンティフィク, 138 (1990)

[Publications] 町田 泰則(分担執筆): "高等植物の情報発現と制御(岡田・池田編)" 丸善株式会社, 292 (1990)

岩渕雅樹京都大学, 理学部, 教授 (30000839)

[Publications] Yamauchi,D.: "Structure of the gene encoding concanavalin A from <Canavalia>_ー <gladiata>_ー and its expression in <Escherichia>_ー <coli>_ー cells" FEBS Lett.260. 127-130 (1990)

[Publications] Akasofu,H.: "Nucleotide sequence of the gene for sulfhydrylーendoーpeptidase (SHーEP) from <Vigna>_ー <mungo>_ー" Nucleic Acids Res.18. 1892 (1990)

[Publications] Tasaka,Masao: "Isolation and characterization of spore coat protein (Sp96) gene of <Dictyostelium>_ー <discoideum>_ー" Cell Diff.Develop.31. 1-9 (1990)

[Publications] Sasaki,Yukiko: "Correlation of plastid DNA copy number with plastid gene expression in various organs in mature pea plants(<Pisum>_ー <sativum>_ー L.)" Plant Cell Physiol.31. 925-931 (1990)

[Publications] Nagano,Yukio: "An essential gene of <Escherichia>_ー <coli>_ー that has sequence similarity to an unknown chloroplast gene" Mol.Gen.Genet.(1991)

[Publications] Yamazaki,Kenーichi: "TGAla,a tobacco DNAーbinding protein,increases the rate of initiation in a plant <in>_ー <vitro>_ー transcription system" Proc.Natl.Acad.Sci.USA. 87. 7035-7039 (1990)

[Publications] Katagiri,F.: "A plant DNAーbinding protein increases the number of active preinitiation complexes in a human <in>_ー <vitro>_ー transcription system" Genes Develop.4. 1899-1909 (1990)

[Publications] 岩渕雅樹(分担執筆): "発生・分化の遺伝子的背景(江口・鈴木・名取編)" 東京大学出版会, 288 (1990)

[Publications] 岩渕雅樹(分担執筆): "高等植物の情報発現と制御(岡田・池田編)" 丸善株式会社, 292 (1990)

[Publications] 岩渕雅樹(分担執筆): "遺伝子の発現と制御II(志村・村松・鈴木・近藤編)" 丸善株式会社, 325 (1990)

[Publications] 岩渕雅樹(分担執筆): "植物全能性の分子生物学(駒嶺他8名編)" 朝倉書店, 305 (1991)

[Publications] 内宮博文: "植物遺伝子操作マニュアルートランスジェニック植物の作り方ー" 講談社サイエンティフィク, 138 (1990)

[Publications] 町田泰則(分担執筆): "高等植物の情報発現と制御(岡田・池田編)" 丸善株式会社, 292 (1990)