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1991 Fiscal Year Final Research Report Summary

Development of Transformation Methods Suitable for Plant Breeding

Research Project

Project/Area Number 02304016
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionNagoya University

Principal Investigator

HIRAI Atsushi  Nagoya U. Fac. Agr. Associate Professor, 農学部, 助教授 (60023470)

Co-Investigator(Kenkyū-buntansha) MORI Koichi  Hiroshima U. Biol. Professor, 農学部, 助教授 (70166354)
MORIKAWA Hiromichi  Hokkaido U. Fac. Agr. Associate Professor, 理学部, 教授 (00089129)
YAMAMOTO Koji  Nagoya T. U. Biol. Professor, 生物系, 教授 (70011971)
UCHIMIYA Hirofumi  Tokyo U. IAM Professor, 応用微生物研究所, 教授 (50142229)
HATTORI Kazumi  Nagoya U. Fac. Agr. Assistant Professor, 農学部, 助手 (40023494)
Project Period (FY) 1990 – 1991
KeywordsTransformation / Particle gun / Laser injection / Ti plasmid / Direct gene transfer / Cell fusion
Research Abstract

Many methods for introducing genes to plants are reported. However, the best method for plant breeding is different from each case. We examined seven transformation methods, and tried to recognize their characteristics. Particle guns were successfully used not only to observe the transient expression of introduced genes, but also to construct transgenic plants. The Laser injection is a new method for transformation and showed the transient expression of a marker gene. The gene transfer was also observed by the grafting method to some extent. Pollens treated with DNA were used to crossing and showed usable for transformation. Ti plasmid mediated gene transfer is popular, and we also found to be functional. Direct gene transfer by PEG was excellent to construct transgenic monocot plants such as rice. Asymmetric cell fusion was convenient to transfer a large amount of genetic information.

  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Honda,H.and A.Hirai: "The gene for the alpha-subunit of ATPase:a site of homologous recombination in plant mitochondrial DNA also functions in somatic hybrid cells." Theor,Appl.Genet.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujii,N.and H,Uchimiya: "Conditions favorable for the somatic embryogenesis in carrot cell culture enhanece expression of the rolC promoter-GUS fusion gene." Plant Physiol.95:. 238-241 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noguchi,T.,Y Hirata and N.Yagishita: "Interspecific chimera formation in Brassica by In Vitro graft method." Theor.Appl.Genet.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toriyama,K.,J.C.Stein.M.Nasrallah & J.Nasrallah: "transformation of Brassica oleracea with an S-locus gene from B.campestris changes the self-incompatibility phenotype." Theor.Appl.Genet.81:. 769-776 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yamashita,A.Iida and H.Morikawa: "Evidence that more than 90% of beta-glucuronidase-expressing cells after particle bombardment directly receive the foreign gene in their nucleus." Plant Physiol.97:. 829-831 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zhou,W.M.,K.Yoshida,Y.Shintaku and G.Takeda: "The use of IAA to overcome interspecific hybrid inviability in reciprocal crosses between Nicotiana tabacum L.and N.repanda Willd." Theor.Appl.Genet.82:. 657-661 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirai, A: "Molecular structure of chloroplast DNA from rice." Biotechnology in Agric. & For.14. 458-467 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, H., K. Itoh and A. Hirai: "The heterogeneous composition of mtDNA in somatic hybrid calli and the relatively simple composition of such DNA in regenerated leaves." Jpn. J. Genet.66. 279-289 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanno, A. and A. Hirai: "Comparative studies on the structure of ctDNA from 4 species of Oryza : Cloning and physical map." Theor. Appl. Genet.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, H. and A. Hirai: "The gene for the alpha-subunit of ATPase : a site of homologous recombination in plant mitochondrial DNA also functions in somatic hybrid cells." Theor. Appl. Genet.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwahashi, M., M. Nakazono, A. Kanno, K. Sugino, T. Ishibashi and A. Hirai: "Genetic and physical maps and a clone bank of mitochondrial DNA from rice." Theor. Appl. Genet.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatano, S. J. Yamaguchi and A. Hirai: "The preparation of high-molecular-weight DNA from rice and its analysis by pulsed-field gel electrophoresis." Plant Sci.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hattori, K.: "Inheritance of carotenoid pigmentation in flower color of chrysanthemum." Japan. J. Breed.41. 1-9 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujii, N. and H. Uchimiya: "Conditions favorable for the somatic embryogenesis in carrot cell culture enhance expression of the rolC promoter-GUS fusion gene." Plant Physiol. 95. 238-241 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanaya, K., K. Hayakawa and H. Uchimiya: "In vitro binding of wheat-germ proteins to the 5'-upstream region of the rolC gene of Ri plasmid." Plant Cell Physiol. 32. 295-297 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oono, Y., T. Satomi and H. Uchimiya: "Agrobacterium rhizogenes lacZ-rolC expression in Escherichia coli : detection of the product in transgenic plants using rolC-specific antibodies" Gene. 104. 95-98 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi, T., Y. Hirata and N. Yagishita: "Interspecific chimera formation in Brassica by In Vitro graft method." Theor. Appl. Genet.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lee, H. Y. and T. Kameya: "Selection and characterization of a rice mutant to 5-methyltryptophan." Theor. Appl. Genet.82. 405-408 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toriyama, K., J. Stein, M. Nasrallah & J. Nasrallah: "Transformation of Brassica oleracea with an S-locus gene from B. canpestris changes the self-incompatibility phenotype." Theor. Appl. Genet.81. 769-776 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kageyama, Y., H. Fukuoka, K. Yamamoto and G. Takeda: "The rice plant bearing endospermless grains : A novel mutant induced by gamma-irradiation of tetra ploid rice (Oryza sativa L.)." Jpn. J. Breed. 41. 341-345 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Iida, M. Seki, M. Kamada, Y. Yamada and H. Morikawa: "Gene delivery into cultured plant cells by DNA-coated gold particles accelerated by a pneumatic particle gun." Theor. Appl. Genet.80. 813-816 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Seki, Y. Komeda, A. Iida, Y. Yamada and H. Morikawa: "Transient expression of beta glucuronidase in Arabidopsis leaves, roots and Brassica napus stems using a pneumatic particle gun." Plant Mol. Biol.17. 259-263 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori K., T, Kinoshita and Y. Yamada: "Callus formation and protoplast isolation in rice and its related species." J. Fac. Agri. Hokkaido Univ. 64. 304-310 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, I.: "New DNA fingerprinting procedure : Amplified fragment length polymorphism of hazy associations (ALPHA)." Genet.41. 105-106 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kishima, Y., M. Suiko and T. Adachi: "Betalain pigmentation in petal of Portulaca is preceded by a dramatic tyrosine accumulation." J. Plant Physiol.137. 505-506 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zhou, W., K. Yoshida, Y. Shintaku and G. Takeda: "The use of IAA to overcome interspecific hybrid inviability in reciprocal crosses between Nicotiana tabacum L. and N. repanda Willd." Theor. Appl. Genet.82. 657-661 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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