• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1992 Fiscal Year Final Research Report Summary

Studies on the requlatory mechanisms of renal glomerular and tubular functions.

Research Project

Project/Area Number 02404036
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionFaculty of Medicine, University of Tokyo

Principal Investigator

KUROKAWA Kiyoshi  Univ.Tokyo, 1st.Dept.of Int.Med., Professor, 医学部(病), 教授 (30167390)

Co-Investigator(Kenkyū-buntansha) TANIGUCHI Shigeo  Univ.Tokyo, 1st.Dept.Int.Med.Assistant professor, 医学部(病), 助手 (50188380)
NAKAO Akihide  Univ.Tokyo, 1st.Dept.Int.Med.Assistant professor, 医学部(病), 助手 (10159056)
NOSAKA Kazuo  Univ.Tokyo, 1st.Dept.Int.Med.Assistant professor, 医学部(病), 助手 (70150274)
WATANABE Tsuyoshi  Univ.Tokyo, 1st.Dept.Int.Med.Associate Professor, 医学部(病), 助教授 (80158641)
Project Period (FY) 1990 – 1992
Keywordsmesangial cells / transforming growth factor beta (TGF-beta) / platelet activating factor / endothelin / calcium / tubulo-glomerular feedback
Research Abstract

The major findings we obtained in this research project are as follows. 1) Mesangial cell expressed TGF-beta mRNA and its expression is stimulated by the addition of fetal calf serum and protein kinase C.Almost all the TGF-beta produced by mesangial cell and released into condition medium is in a latent form. Exogenously added TGF-beta inhibited mesangial cell growth. 2) A single class of PAF receptor is present in the quinea pig kidney which is most abundant in the cortex 3) Cloned renal tubule cells, LLC-PK1 cells, express a single entity of PAF receptor mRNA and PAF induced a dose-dependent calcium transients in LLC-PK1 cells. 4) Endothelin-1 dose-dependently increased [Ca^<++>]i both in cortical and medullary collecting duct. Endothelin-1 mRNA was found to be expressed only in cortical and medullary collecting ducts in addition to glomeruli and endothelin-1 binding occurs significantly in glomeruli and collecting tubules. 5) We proposed our hypothesis concerning the transcellular signal transduction in tubulo-glomerular feedback based on the observations we obtained in cultured mesangial cells.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] S.Uchida: "Detection of endothelin-1 mRNA by PCR in isolated rat renal tubules." Biochem.Biophys.Res.Comm.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Nosaka: "Permeable type I collagen membrane promotes glomerular epithelial cell growth in culture" Kidney International.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Kaname: "Autocrine secretion of transtorming growth factor-β in cultured rat mesangial cells." Kidney International.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.M, Asef Jamil: "Expression of platetet activating factor receptor in renal tubular cell line (LLC-PK1)" Biochem.Biophys.Res.Comm.187. 767-772 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsunaga: "Ion channel activities of cultured rat mesangial cells." Am.J.Physiol.30. F808-814 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Naruse: "Endothelin l increases cell calcium in mouse collecting tubule cells" Am.J.Physiol.30. F720-F725 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Kurokawa: "“mechanisms and sites of hormone action in the kidney." in “The Kidney Physiology and Pathophyisology" 2nd edition edcted by DW Seldin and G Giebisch." Reven Press, New York, PP1339-1372 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Uchida: "Detedtion of endothelin-1 mRNA by PCR in isolated rat renal tubules." Biochem.Biophys.Res.Comm.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Nosaka: "Permeable typeI collagen membrane promotes glomerular epithelial cell growth in cuture." Kidney International. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kaname: "Autocrine secretion of transforming growth factor-beta in cultured rat mesangial cells." Kidney International. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.M.Asif.Jamil.: "Expression of platelet activating factor receptor in renal tublar cell line (LLC-PK1)." Biochem.Biophys.Res.Comm.187. 767-772 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsunaga: "Ion chanel activities of cultured rat mesangial cells." Am.J.Physiol.30. F808-814 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Naruse: "Endothelin1 increases cell calcium in mouse collecting tubule cells." Am.J.Physiol.30. F720-725 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kurokawa: "Mechanisms and sites of hormone action in the Kidney." in "The Kidney : Physiology and Pathophyisoloty" edition edcted by DW Seldin and G.Giebisch.Raven Press, New York, 1339-1372 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi