1992 Fiscal Year Final Research Report Summary
Studies on the requlatory mechanisms of renal glomerular and tubular functions.
| Project/Area Number |
02404036
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | Faculty of Medicine, University of Tokyo |
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Principal Investigator |
KUROKAWA Kiyoshi Univ.Tokyo, 1st.Dept.of Int.Med., Professor, 医学部(病), 教授 (30167390)
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| Co-Investigator(Kenkyū-buntansha) |
TANIGUCHI Shigeo Univ.Tokyo, 1st.Dept.Int.Med.Assistant professor, 医学部(病), 助手 (50188380)
NAKAO Akihide Univ.Tokyo, 1st.Dept.Int.Med.Assistant professor, 医学部(病), 助手 (10159056)
NOSAKA Kazuo Univ.Tokyo, 1st.Dept.Int.Med.Assistant professor, 医学部(病), 助手 (70150274)
WATANABE Tsuyoshi Univ.Tokyo, 1st.Dept.Int.Med.Associate Professor, 医学部(病), 助教授 (80158641)
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| Project Period (FY) |
1990 – 1992
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| Keywords | mesangial cells / transforming growth factor beta (TGF-beta) / platelet activating factor / endothelin / calcium / tubulo-glomerular feedback |
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| Research Abstract |
The major findings we obtained in this research project are as follows. 1) Mesangial cell expressed TGF-beta mRNA and its expression is stimulated by the addition of fetal calf serum and protein kinase C.Almost all the TGF-beta produced by mesangial cell and released into condition medium is in a latent form. Exogenously added TGF-beta inhibited mesangial cell growth. 2) A single class of PAF receptor is present in the quinea pig kidney which is most abundant in the cortex 3) Cloned renal tubule cells, LLC-PK1 cells, express a single entity of PAF receptor mRNA and PAF induced a dose-dependent calcium transients in LLC-PK1 cells. 4) Endothelin-1 dose-dependently increased [Ca^<++>]i both in cortical and medullary collecting duct. Endothelin-1 mRNA was found to be expressed only in cortical and medullary collecting ducts in addition to glomeruli and endothelin-1 binding occurs significantly in glomeruli and collecting tubules. 5) We proposed our hypothesis concerning the transcellular signal transduction in tubulo-glomerular feedback based on the observations we obtained in cultured mesangial cells.
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