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1991 Fiscal Year Final Research Report Summary

The Role of Protein Phosphorylation and dephosphorylation in Neuronal Signal Transduction

Research Project

Project/Area Number 02454137
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKobe University

Principal Investigator

TANAKA Chikako  Kobe Univ. Sch. Med., Professor, 医学部, 教授 (20025571)

Co-Investigator(Kenkyū-buntansha) SHUNTOH Hisato  Kobe Univ. Sch. Med., Research Associate, 医学部, 助手 (70206259)
SAITO Naoaki  Kobe Univ. Sch. Med., Associate Professor, 医学部, 講師 (60178499)
KUNO Takayoshi  Kobe Univ. Sch. Med., Associate Professor, 医学部, 助教授 (50144564)
Project Period (FY) 1990 – 1991
KeywordsProtein Phosphorylation / Dephosphorylation / Protein Kinase / Calcineurin / Synapse Formation / Neurotransmitter / cDNA Cloning
Research Abstract

Recently, protein phosphorylation and dephosphorylation are thought to be a central event in cellular signal transduction. Large amount of protein kinases and protein phosphatases are found in the central nervous system, suggesting that these enzymes are involved in the neuronal signal transduction. In this fiscal year, we obtained following findings on the protein kinase C and calcineurin.
1) Developmental change in the distribution of protein kinase C subspecies in rat brain was examined. alpha and beta I subspecies were found when rat was born, on the other hand, beta II and gamma subspecies appeared one to two weeks after the birth when the synaptogenesis occurred.
2) Immunohistochemical study on the localization of four protein kinase C subspecies and neurotransmitters in striate-nigral system was performed. Dopaminergic neurons in the substantia nigra and cholinergic neurons in the striatum contained alpha subspecies, and GABAergic neurons in the striatum contained beta -I subspecies.
3) Distribution of calcineurin A subtypes were examined using in situ hybridization technique and immunohistochemistry in rat brain. The activation mechanism of calcineurin was studied usign calmodulin antagonists.
4) GABA_B receptor was expressed in Xenopus oocyte and its regulation by protein kinase C was demonstrated.
5) cDNA cloning of testis-specific calcineurin B-like protein and cDNA cloning of yeast homolog of calcineurin B were performed.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] M.Hirata: "Differential expression of the βI- and βII-PKC subspecies in the postnatal developing rat brain;an immunocytochemical study." Dev.Brain Research. 62. 229-238 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.Yoshihara: "Differential localization of four subspecies of protein kinase C in the rat striatum and substantia nigra." J.neurosci.11. 690-700 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Mukai: "Calmodulin antagonists differentiate between Ni^<2+>-and Mn^<2+>- stimulated phosphatase activity of calcineurin." J.Biochem.110. 402-406 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Takaishi: "Differential distribution of the mRNA encoding two isoforms of the catalytic subunit of calcineurin in the rat brain." Biochem.Biophys.Res.Commun.174. 393-398 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Taniyama: "Expression of the GABA^B receptor in Xenopus oocytes and inhibition of the response by activation of protein kinase C." FEBS Lett.278. 222-224 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kuno: "cDNA cloning of a calcineurin B homolog in saccharomyces cerevosoae." Biochem.Biophys.Res.Commun.180. 1159-1163 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Taniyama: "Advances in Experimental Medicine and Biology" Plenum Press, 8 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田中 千賀子: "〈代謝〉シグナル伝達路とそのクロスト-ク" 中山書店, 11 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Hirata: "Differential expression of the beta I-and beta II-PCK subspecies in the postnatal developing rat brain" Dev. Brain Research. 62. 229-238 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C. Yoshihara: "Differential localization of four subspecies of protein kinase C in the rat striatum and substantia nigra. an immunocytochemical study." J. Neurosci.11. 690-700 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Mukai: "Calmodulin antagonists differentiate between Ni^<2+>-and Mn^<2+>-stimulated phosphatase activity of calcineurin." J. Biochem.110. 402-406 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Takaishi: "Differential distribution of the mRNA encoding two isoforms of the catalytic subunit of calcineurin in the rat brain." Biochem. Biophys. Res. Commun.174. 393-398 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Taniyama: "Expression of the GABA_B receptor in Xenopus oocytes and inhibition of the response by activation of protein kinase C." FEBS Lett.278. 222-224 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Kuno: "cDNA cloning of a calcineurin B homolog in saccharomyces cerevisiae." Biochem. Biophys. Res. Commun.180. 1159-1163 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Taniyama: "Expression of the GABA_B receptor in Xenopus oocytes and desensitization by activation of protein kinase C." Adv. Experimental Med. Biol.287. 413-420 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C. Tanaka: "The review on signal transduction and crosstalks. (Signal Transduction and Crosstalks)" Taisha. 374. 1-10 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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