1991 Fiscal Year Final Research Report Summary
Novel Mitochondrial fatty Acid Oxidation Enzymes
| Project/Area Number |
02454144
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Shinshu University |
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Principal Investigator |
HASHIMOTO Takashi Shinshu University, Sch. of Med., Professor, 医学部, 教授 (80009935)
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| Co-Investigator(Kenkyū-buntansha) |
FURUTA Shuichi Shinshu University, Sch. of Med., Assistant, 医学部, 助手 (80126705)
MIYAZAWA Shoko Shinshu University, Sch. of Med., Assistant, 医学部, 助手 (20020745)
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| Project Period (FY) |
1990 – 1991
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| Keywords | mitochondria / fatty acid oxidation enzymes / inborn errors of metabolism |
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| Research Abstract |
1. We found two novel fatty acid oxidation enzymes in rat liver mitochondria, and purified. We named very long-chain acyl-CoA dehydrogenase and enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolise trifunctional protein, respectively. The molecular and catalytic properties of these enzymes are quite different from those of the known enzymes. The latter enzyme has been called as long-chain 3-hydroxyacyl-CoA dehydrogenase.
2. We have obtained human and rat EDNA clones for these two enzymes.
3. We have first described the presence long-chain 3-hvdroxyacyl-CoA dehydrogenase (trifunctional protein) deficiency.
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