1992 Fiscal Year Final Research Report Summary
Molecular, cellular and pathological analysis of structure and function of macrophages
| Project/Area Number |
02454169
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Oita Medical University |
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Principal Investigator |
YAMAMOTO Shunsuke Oita Medical Univ.,Pathology,Professor, 医学部, 教授 (90040188)
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| Co-Investigator(Kenkyū-buntansha) |
SETOGUCHI Mihoko Oita Medical Univ.,Pathology,Assistant, 医学部, 助手 (20236110)
YOSHIDA Seiji Oita Medical Univ.,Pathology,Assistant, 医学部, 助手 (70182764)
AKIZUKI Shinishiro Oita Medical Univ.,Pathology,Assistant, 医学部, 助手 (80159334)
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| Project Period (FY) |
1990 – 1992
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| Keywords | CD14 / MS2 / osteopontin / cDNA / genome / expression / recombinant / mouse and human |
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| Research Abstract |
1.MS2:(1). Complete nucleotide sequences of the MS2 gene and its 5 upstream region were determined. (2). cis-regulatory element for MS2 expression in macrophages (Mphi) was investigated. (3). Significant homology of MS2 with hemorrhagic snake venom proteins were found. (4). Recombinant proteins for MS2 proteins (rMS2) and poly clonal and monoclonal antibodies for rMS2 (anti- rMS2) were produced. (5). PAP and FACS analysis revealed that MS2 was expressed in Mphi and neutrophils but not in lymphocytes and mast cells. (6). cDNA for the human homologue of MS2 was separated.
2. CD14:(1). The 5'upstream nucleotide sequences of the mouse and human CD14 genes were determined. (2). Promoter and enhancer regions for the mouse CD14 gene were defined. (3). Mouse rCD14 and polyclonal and monoclonal antibodies against rCD14 (anti- rCD14) were produced. PAP analysis using anti-rCD14 demonstrated CD14 in myelomonocytic cells but not in lymphocytes and mast cells. (5). TNFalpha production and LPS-induced shock were significantly suppressed with anti-rCD14.
3. OP:(1). The 5'upstream nucleotide sequences of the mouse and human OP genes were determined. (2). Complete nucleotide sequence of the human OP gene was determined. (3). Cis regulatory sequences of the mouse and human OP genes were determined. (4). rOP was produced using E. coli and vaculovirus vector systems. (5). rOP significantly suppressed haptotaxis of Mphi and melanoma (B16-BL) cells, and lung metastasis of B16-BL cells. (5). Antibodyagainst rOP was produced.
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[Publications] Nasu, N., Yoshida, S., Akizuki, S., Higuchi, Y., Setoguchi, M. and Yamamoto,S: "Molecular and physiological properties of murine CD14." Int. Immunol. 3(2). 205-213 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Matsuura, K., Ishida, T., Setoguchi, M., Higuchi, U., Akizuki, S., and Yamamoto, S.: "Identification of a tissue-specific regulatory element within the murine CD14 gene" J. Biol. Chem.267(30). 21718-21794 (1992)
Description
「研究成果報告書概要(欧文)」より
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