HTLV-I infection and immune disorders : A role of HTLV-I tax gene for mesenchymal cell transformation.

1992 Fiscal Year Final Research Report Summary

• Project/Area Number: 02454225
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: 内科学一般
• Research Institution: Rheumatology Division, Institute of Medical Science, St Marianna University (1991-1992); Tokyo Women's Medical University (1990)
• Principal Investigator: NISHIOKA Kusuki St. Marianna Univ.:Professor of Medicine, 医学部, 教授 (60049070)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Kazuhiko Univ. of Tokyo :MD, 医学部, 講師 (80191394)
• Project Period (FY): 1990 – 1992
• Keywords: HTLV-I associated arthropathy / synovial hyperplasia / proto oncogene / colony formation / genomic transfer / loss of contact inhibition / retrovirus / rheumatoid arthritis

Research Abstract

The etiological factor(s) of rheumatoid arthritis (RA) have been well unknown.
The salient features is invasive synovial proliferation lead to bone erosion.
We have revealed the relationship of the HTLV-I infection to chronic arthropathy through out the study of clinical and molecular biological analysis and established the following results.
1) The patient with arthropathy in HTLV-I infection was proposed as one of the subsets of RA.
2) The presence and the expression of proviral HTLV-I genome in synovial cell derived from HAAP patient were clarified.
3) HTLV-I tax expression causes synovial transformation and hyperplasia in either human synovial or transgenic mice. Therefore, we demonstrate that HTLV-I tax gene is the first exogeneous retroviral gene which causes resembling rheumatoid arthritis.

Research Products

• [Publications] N.MIYASAKA,M.HIGAKI,K.SATO,J.HASHIMOTO,A.TANIGUCHI,H.KOHSAKA,K.TAMAMOTO,K.SHICHIKAWA,K.NISHIOKA: "Production of Interleukin-1 β-like Factor with Synovial Cell Growth Promoting Activity from Adult T-cell Leukemia Cells" Journal Autoimmunity. 4. 223-236 (1991)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K.SATO,I.MARUYAMA,Y.MARUYAMA,I.KITAJIMA,Y.NAKAJIMA,Y.NAKAJIMA,M.HIGAKI,M.OSAME,K.NISHIOKA: "Arthritis in Patients Infected with Human T Lymphotropic Virus Type-1.Clinical and Immunopathologic Features." Arthritis & Rheumatism. 34(6). 715-721 (1991)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y.IWAKURA,M.TOSU,E.YOSHIDA,M.TANIGUCHI,K.SATO,I.KITAJIMA,K.NISHIOKA,K.YAMAMOTO,T.TAKEDA,M.HATANAKA,H.YAMAMOTO,T.SEKIGUCHI: "Induction of Inflammatory Arthropathy Resembling Rheumatoid Arthritis in Mice Transgenic for HTLV-I" Science. 253. 1026-1029 (1991)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] I.KITAJIMA,K.YAMAMOTO,K.SATO,Y.NAKAJIMA,T.NAKAJIMA,I.MARUYAMA,M.OSAME,K.NISHIOKA: "Detection of Human T Cell Lymphotropic Virus Type-1 Proviral DNA and Its Gene Expression in Synovial Cells in Chronic Inflammatory Arthropathy" The Journal of Clinical Investigation. 88(4). 1315-1322 (1991)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y.YAMAMOTO,H.SAKODA,T.KATO,M.OKUBO,M.DOHI,Y.MIZUSHIMA,K.ITO,K.NISHIOKA: "Accumulation of multiple T cell clonotypes in the synovial lesions of patients with rheumatoid arhtritis revealed by a novel clonality analysis." International Immunology. 4(11). 1219-1223 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T.NAKAJIMA,H.AONO,T.HASUNUMA,K.TAMAMOTO,I.MARUYAMA,T.NOSAKA,M.HATANAKA,K.NISHIOKA: "Overgrowth of Human Synovial Cells Driven by HTLV-I tax gene." Journal of Clinical Investigation. (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] N.MIYASAKA, M.HIGAKI, K.SATO, J.HASHIMOTO, A.TANIGUCHI, H.KOHSAKA, K.TAMAMOTO, K.SHICHIKAWA, K.NISHIOKA: "Production of Interleukin-1 beta-like Factor with Synovial Cell Growth Promoting Activity from Adult T-cell Leukemia Cells" The Journal of Autoimmunity. 4. 223-236 (1991)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K.SATO, I.MARUYAMA, Y.MARUYAMA, I.KITAJIMA, Y.NAKAJIMA, M.HIGAKI, K.YAMAMOTO, N.MIYASAKA, M.OSAME, K.NISHIOKA: "Arthritis in Patients Infected with Human T Lymphotropic Virus Type-1.Clinical and Immunopathologic Features." Arthritis & Rheumatism. 34(6). 715-721 (1991)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Y.IWAKURA, M.TOSU, E.YOSHIDA, M.TANIGUCHI, K.SATO, I.KITAJIMA, K.NISHIOKA, K.YAMAMOTO, T.TAKEDA, M.HATANAKA, H.YAMAMOTO, T.SEKIGUCHI: "Induction of Inflammatory Arthropathy Resembling Rheumatoid Arthritis in Mice Transgenic for HTLV-I" Science. 253. 1026-1029 (1991)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] I.KITAJIMA, K.YAMAMOTO, K.SATO, Y.NAKAJIMA, T.NAKAJIMA, I.MARUYAMA, M.OSAME, K.NISHIOKA: "Detection of Human T Cell Lymphotropic Virus Type-1 Proviral DNA and Its Gene Expression in Synovial Cells in Chronic Inflammatory Arthropathy" The Journal of Clinical Investigation. 88(4). 1315-1322 (1991)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Y.YAMAMOTO, H.SAKODA, T.NAKAJIMA, T.KATO, M.OKUBO, M.DOHI, Y.MIZUSHIMA, K.ITO, K.NISHIOKA: "Accumulation of multiple T cell clonotypes in thr synovial lesions of patients with rheumatoid arthtritis revealed by a novel clonality analysis." International Immunology. 4(11). 1219-1223 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] T.NAKAJIMA, H.AONO, T.HASUNUMA, K.TAMAMOTO, I.MARUYAMA, T.NOSAKA, M.HATANAKA, K.NISHIOKA: "Overgrowth of Human Synovial Cells Driven by HTLV-I tax gene." The Journal of Clinical Investigation. (1993)
  • Description: 「研究成果報告書概要(欧文)」より