1991 Fiscal Year Final Research Report Summary
Analysis of human D2 dopamine receptor gene in schizophrenia
| Project/Area Number |
02454291
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Psychiatric science
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| Research Institution | Niigata University |
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Principal Investigator |
KUWANO Ryozo Niigata University, Research Laboratory for Molecular Genetics, Associate Professor, 遺伝子実験施設, 助教授 (20111734)
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| Co-Investigator(Kenkyū-buntansha) |
MORII Ken Niigata University, Research Laboratory for Molecular Genetics, Assistant, 遺伝子実験施設, 助手 (20230089)
ABE Satoshi Niigata University, Brain Research Institute, Assistant, 脳研究所, 助手 (90202663)
USUI Hiroshi Niigata University, Brain Research Institute, Assistant, 脳研究所, 助手 (20192510)
KUMANISHI Toshiro Niigata University, Brain Research Institute, Professor, 脳研究所, 教授 (40018601)
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| Project Period (FY) |
1990 – 1991
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| Keywords | D2 dopamine receptor / Gene Schizophrenia / Polymorphism / SSCP / SSCP法 |
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| Research Abstract |
Dopamine and dopamine receptors may be involved in the movement and emotion balances. D2 dopamine receptor has high affinity for the antipsychotic drugs. Tliis receptor may also be related to the pathoctlenesis of Parkinson's disease and schizophrenia. We purified the bovine brain D2 dopamine receptor protein by photoaffinity labeling, high performance liquid chrom-atoaraphy and preparative sodium dodecyl sulphate polyacrylamide gel electrophoresis. Further we analyzed electrophysiologically the D2 dopamine receptor expressed in Xenopus Oocyte after injection of poly(A)RNA. We attempted to analyze the structure of the D2 dopamine receptor gene in schizophrenia. We succeeded in cloning the genomic DNA for D2 dopamine receptor and clarified the structure of this gene. This gene spans 15 kbp and consists of 7 exons interrupted by 6'introns. Spot-blot hybridization analysis of fractionated human chromosomal DNA with D2 receptor "enomic DNA revealed the localization of this gene in the chromosome I 1. We analyzed human genomic DNA by Southem blot analysis, but so far we could not find restriction fragment length polymorphism(RFLP)in schizophrenia. We are trying to detect the differences of the sequences of dopamine D2 receptor gene between normal and schizophrenia by single strand conformation polymorphism(SSCP).
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